Enhanced sucrose loading improves rice yield by increasing grain size

Wang, Liang; Lu, Qingtao; Wen, Xiaopeng; Lu, Congming

doi:10.1104/pp.15.01170

Cited by 79 publications

(79 citation statements)

References 70 publications

(95 reference statements)

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“…Therefore, a direct link between SUT expression and phloem loading rate does not seem to exist for the up-regulation of phloem loading under these conditions. This contrasts with studies that artificially up-regulate a relevant SUT through overexpression, which, in some cases, did result in increased phloem loading (Dasgupta et al, 2014;Wang et al, 2015).…”

Section: Sut Expression Does Not Always Correspond To Phloem Loadingcontrasting

confidence: 43%

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Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

et al. 2017

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Suc transporters (SUTs) play a key role in the allocation and partitioning of photosynthetically fixed carbon in plants. While a function could be assigned to many members of the SUT family, almost no information is available on their regulation. Here, the transcriptional regulation of SUTs in response to various environmental stimuli in the leaves of five dicots (Arabidopsis Extensive data on expression of SUTs in relation to changes of environmental conditions were obtained through a global analysis of 168 transcriptomics data sets. Results were validated by quantitative PCR measurements and extended by the measurement of photosynthesis rate and phloem sugar content to draw insight on the correlation of SUT expression and sugar export from leaves. For the apoplasmic phloem loaders, a clear difference in transcriptional regulation in response to different environmental stimuli was observed. The consistent patterns of SUT expression under abiotic stress indicates which types of SUTs are involved in the regulation of leaf sugar status and in stress signaling. Furthermore, it is shown that down-regulation of phloem loading is likely to be caused by transcriptional regulation of SUTs, while up-regulation depends on post-transcriptional regulation. In poplar, expression of PtaSUT4 was found to consistently respond to environmental stimuli, suggesting a significant role in the regulation of sugar export from leaves in this passive symplasmic phloem loader.

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Section: Sut Expression Does Not Always Correspond To Phloem Loadingcontrasting

confidence: 43%

“…A connection between SUT transcription and transport activity became evident through genetic manipulations of SUT expression (Bürkle et al, 1998;Slewinski et al, 2009;Wang et al, 2015). Several studies supported this link in nonmanipulated plants.…”

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confidence: 64%

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

et al. 2017

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“…Increased expression of SUT1 , the sucrose transporter critical for cell‐to‐cell sucrose movement and sucrose phloem loading (Srivastava et al ., 2008; Wang et al ., 2015), was also demonstrated in the roots using qRT‐PCR (Figure 11). Other genes, such as α ‐amylase , INV1 , INV2 , SUS and SUT2 , were not affected in IA lines under either condition.…”

Section: Resultsmentioning

confidence: 89%

H⁺‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]

Fan

Wang

et al. 2017

Plant Biotechnology Journal

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SummaryIron (Fe) deficiency is one of the most common micronutrient deficiencies limiting crop production globally, especially in arid regions because of decreased availability of iron in alkaline soils. Sweet potato [Ipomoea batatas (L.) Lam.] grows well in arid regions and is tolerant to Fe deficiency. Here, we report that the transcription of type I H+‐pyrophosphatase (H+‐PPase) gene IbVP1 in sweet potato plants was strongly induced by Fe deficiency and auxin in hydroponics, improving Fe acquisition via increased rhizosphere acidification and auxin regulation. When overexpressed, transgenic plants show higher pyrophosphate hydrolysis and plasma membrane H+‐ATPase activity compared with the wild type, leading to increased rhizosphere acidification. The IbVP1‐overexpressing plants showed better growth, including enlarged root systems, under Fe‐sufficient or Fe‐deficient conditions. Increased ferric precipitation and ferric chelate reductase activity in the roots of transgenic lines indicate improved iron uptake, which is also confirmed by increased Fe content and up‐regulation of Fe uptake genes, e.g. FRO2,IRT1 and FIT. Carbohydrate metabolism is significantly affected in the transgenic lines, showing increased sugar and starch content associated with the increased expression of AGPase and SUT1 genes and the decrease in β‐amylase gene expression. Improved antioxidant capacities were also detected in the transgenic plants, which showed reduced H2O2 accumulation associated with up‐regulated ROS‐scavenging activity. Therefore, H+‐PPase plays a key role in the response to Fe deficiency by sweet potato and effectively improves the Fe acquisition by overexpressing IbVP1 in crops cultivated in micronutrient‐deficient soils.

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“…Additionally, phloem loading also affect NSC translocation (Wang et al 2015b). Sugar phloem loading occurs primarily through apoplastic and symplastic pathways (Braun et al 2014).…”

Section: Analyzing the Primary Limiting Factors To Nsc Translocation mentioning

confidence: 99%

Potassium deficiency aggravates yield loss in rice by restricting the translocation of non‐structural carbohydrates under Sarocladium oryzae infection condition

Zhang

Pan

et al. 2019

Physiologia Plantarum

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Sheath rot disease (ShR) caused by Sarocladium oryzae (S. oryzae) infection is an emerging disease that causes severe yield loss by restricting the translocation of non‐structural carbohydrates (NSC). Potassium (K) nutrition plays a critical role in disease resistance and the exportation of NSC. However, the physiological mechanisms of K with respect to ShR have not been thoroughly elucidated to date. The objectives of this study were to reveal the mechanisms by which K increases ShR resistance by regulating NSC translocation of rice, therefore, a field experiment combined with an inoculation experiment was conducted. We demonstrate that ShR disease incidence and disease index decreased dramatically with an increasing K application. K deficiency sharply induced the accumulation of NSC in the flag leaf (FL) and flag leaf sheath (FLS) under S. oryzae infection condition, which reduced the contribution of transferred NSC to final yield. A permutational multivariate analysis showed that K deficiency had a greater (49.0%, P < 0.001) effect on the NSC content variation in FL than that of S. oryzae infection (15.0%, P < 0.001). S. oryzae infection dramatically increased the difference in apparent transferred mass of NSC and cell membrane injury of diseased organs between K‐deficient and K‐sufficient rice. Finally, we demonstrate that cell membrane injury was a limiting factor imposed by K deficiency, which restricts the export of NSC from source organs. This work highlights the importance of K in improving ShR resistance by regulating NSC translocation (particularly the stem NSC).

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Enhanced sucrose loading improves rice yield by increasing grain size

Cited by 79 publications

References 70 publications

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

H⁺‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]

Potassium deficiency aggravates yield loss in rice by restricting the translocation of non‐structural carbohydrates under Sarocladium oryzae infection condition

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Enhanced sucrose loading improves rice yield by increasing grain size

Cited by 79 publications

References 70 publications

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

H+‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]

Potassium deficiency aggravates yield loss in rice by restricting the translocation of non‐structural carbohydrates under Sarocladium oryzae infection condition

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H⁺‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]