Enhanced thoracic gene delivery by magnetic nanobead‐mediated vector

Li, Wenzhong; Ma, Nan; Ong, Lawrence; Kaminski, Alexander; Skrabal, Christian; Uğurlucan, Murat; Lorenz, Peter; Gatzen, H.H.; Lützow, Karola; Lendlein, Andreas; Pützer, Brigitte M.; Li, Ren‐Ke; Steinhoff, Gustav

doi:10.1002/jgm.1208

Cited by 64 publications

(50 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Li et al optimized the conjugation of PEI onto the surface of the magnetic nanoparticles. The magnetic nanoparticles could enhance the transfection efficiency and effectively deliver genes to the left side of the mouse thorax underexternal magnetic guidance (Li et al 2007b(Li et al , 2008.…”

Section: Applications For Therapeutic Devicesmentioning

confidence: 99%

Functionalized Nanomaterials

Zhou

Gao

2010

Regenerative Medicine

Self Cite

View full text Add to dashboard Cite

Regenerative medicine aims to repair tissues or organs for restoring normal functions, which represents one of the greatest challenges in modern science and medicine. Diverse techniques and materials are required to truly understand the process of tissue repairing and build a proper scaffold for cells attachment, proliferation and differentiation. Functionalized nanomaterials with nanotechnologies are the ideal to solve most of the problems of regenerative medicine. Multifunctionalized nanoparticles and nanostructured biomaterials can be powerful tools for cell tracking and matrix-like scaffold rebuilding.

show abstract

Section: Applications For Therapeutic Devicesmentioning

confidence: 99%

Functionalized Nanomaterials

Zhou

Gao

2010

Regenerative Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Under general anesthesia, after the placement of a 20g intravenous catheter into the distal cephalic vein, for the forearm, or into the small saphenous vein, for the hind limb, a tourniquet, above the elbow or the knee, is inflated to a pressure of 450 mmHg. The gene solution is rapidly infusion at a rate of 2 ml/sec and 2 minutes post-injection the tourniquet is deflated 55,72,73,30,31,32 .…”

Section: Primatesmentioning

confidence: 99%

Animal Models for Hydrodynamic Gene Delivery

Katsimpoulas¹,

Zacharoulis²,

Habib³

et al. 2012

Chemical Biology

View full text Add to dashboard Cite

236The vehicle for the molecules is Normal Saline, Ringer's Solution or Phosphate Buffered Saline and the dosage range from 0.1 to 10 mg/kg, depending on the application. The molecules can be DNA, as plasmid, fragment or artificial chromosome, RNA, single or double stranded, genomic or not, polymers, proteins and small compounds. The main application of the hydrodynamic delivery is the therapy studies, especially genes encoding secretory proteins which can be even isolated and purified. A different area of interest is the gene function analysis in a whole animal instead of a cell culture; the transfection is applied with the hydrodynamic technique and the conclusions are withdrawn from the whole animal. The study of DNA sequences can be achieved with HGD, where promoters, introns, enhancers, suppressors can be delivered intracellular in whole animal. Moreover new animal models are possible to be created for study human diseases, mainly viral due to the fact that they are species specific 5,8,10 .

show abstract

“…Li et al (56) reported that transfection efficiency of PEI/DNA polyplexes, which are covalently linked to dextran-modified magnetic beads (200 nm), shows 35- to 85-fold higher values under magnetic field. Similarly, Xenariou et al (57) demonstrated reporter gene expression of Lipofectamine 2000/pDNA lipoplex increase 300-fold under a magnetic field, when complexed with TransMAG PEI (consisting of iron oxide cores and PEI shell) at suboptimal pDNA concentrations.…”

Section: Nanomagnets In Nanomedicinementioning

confidence: 99%

Iron oxide-based nanomagnets in nanomedicine: fabrication and applications

Lin

Kim

et al. 2010

Nano Reviews

View full text Add to dashboard Cite

Iron oxide-based nanomagnets have attracted a great deal of attention in nanomedicine over the past decade. Down to the nanoscale, superparamagnetic iron oxide nanoparticles can only be magnetized in the presence of an external magnetic field, which makes them capable of forming stable colloids in a physio-biological medium. Their superparamagnetic property, together with other intrinsic properties, such as low cytotoxicity, colloidal stability, and bioactive molecule conjugation capability, makes such nanomagnets ideal in both in-vitro and in-vivo biomedical applications. In this review, a chemical, physical, and biological synthetic approach to prepare iron oxide-based nanomagnets with different physicochemical properties was illustrated and compared. The growing interest in iron oxide-based nanomagnets with multifunctionalities was explored in cancer diagnostics and treatment, focusing on their combined roles in a magnetic resonance contrast agent, hyperthermia, and magnetic force assisted drug delivery. Iron oxides as magnetic carriers in gene therapy were reviewed with a focus on the sophisticated design and construction of magnetic vectors. Finally, the iron oxide-based nanomagnet also represents a very promising tool in particle/cell interfacing in controlling cellular functionalities, such as adhesion, proliferation, differentiation, and cell patterning, in stem cell therapy and tissue engineering applications.

show abstract

Enhanced thoracic gene delivery by magnetic nanobead‐mediated vector

Abstract: MNB-mediated gene delivery could comprise a promising method for gene delivery to the lung and the heart.

Cited by 64 publications

References 54 publications

Functionalized Nanomaterials

Functionalized Nanomaterials

Animal Models for Hydrodynamic Gene Delivery

Iron oxide-based nanomagnets in nanomedicine: fabrication and applications

Contact Info

Product

Resources

About