Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco (Nicotiana tabacum) chloroplasts

Badawi, Ghazi Hamid; Yamauchi, Yasuo; Shimada, Emi; Sasaki, Ryozo; Kawano, Naoyoshi; Tanaka, Kunisuke; Tanaka, Kiyoshi

doi:10.1016/j.plantsci.2003.12.007

Cited by 281 publications

(34 citation statements)

References 47 publications

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“…It may be suggested that the synthesis of the new SOD isozyme might contribute to increased SOD activity under salt stress. Similar results have been reported using transgenic plants (Tanaka et al 1999;Badawi et al 2004;Wang et al 2004). At 20 days of stress exposure, two new SOD isozymes of R m 0.46 and R m 0.48 were detected for the salttolerant genotype Gebeina, but no new SOD isozyme was detected for Quzhou.…”

Section: Discussionsupporting

confidence: 87%

Genotypic difference in response of peroxidase and superoxide dismutase isozymes and activities to salt stress in barley

et al. 2009

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Difference in isozymes and activities of peroxidase (POD) and superoxide dismutase (SOD) in two barley (Hordeum vulgare L.) genotypes differing in salt tolerance (Gebeina, tolerant; Quzhou, sensitive) was investigated using a hydroponic experiment. The activities of both enzymes were significantly increased when the plants of the two barley genotypes were exposed to salt stress, with salt-tolerant genotype being generally higher than the sensitive one. The variation in the POD and SOD isozymes was dependent on barley genotype, salt level and exposure time. When the plants were exposed to salt stress for 10 days, two new POD isozymes were found, R m 0.26 (R m , relative mobility of enzyme to dye) in Gebeina and R m 0.45 in Quzhou. Both isozymes disappeared after 20 days of salt stress, but R m 0.26 appeared again 30 days after the stress. Two new SOD isozymes of R m 0.19 and R m 0.46 were found in Gebeina when exposed to NaCl for 10 days, but only R m 0.46 in Quzhou. As the time of salt stress extended, more new SOD isozymes were detected, R m 0.35 in both genotypes in all different salt treatments and R m 0.48 in Gebeina under 200 mM NaCl stress. At 30 days after the stress, all the new SOD isozymes disappeared except for R m 0.48 in Gebeina under 200 mM NaCl stress. The results suggest that the increased POD and SOD activities could be partly due to the formation of some new isozymes and the tolerant variety had better ability to form new isozymes to overcome salt stress.

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Section: Discussionsupporting

confidence: 87%

Genotypic difference in response of peroxidase and superoxide dismutase isozymes and activities to salt stress in barley

et al. 2009

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“…The overexpression of Cu/Zn SOD in chloroplasts of Arabidopsis [16], tobacco [17,18], Chinese cabbage ( Brassica campestris L. ssp. pekinensis cv.…”

Section: Salinity-induced Diverse Ros Scavenging Pathways In Chlormentioning

confidence: 99%

Salinity Response in Chloroplasts: Insights from Gene Characterization

Suo

Zhao

David

et al. 2017

IJMS

113

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Salinity is a severe abiotic stress limiting agricultural yield and productivity. Plants have evolved various strategies to cope with salt stress. Chloroplasts are important photosynthesis organelles, which are sensitive to salinity. An understanding of molecular mechanisms in chloroplast tolerance to salinity is of great importance for genetic modification and plant breeding. Previous studies have characterized more than 53 salt-responsive genes encoding important chloroplast-localized proteins, which imply multiple vital pathways in chloroplasts in response to salt stress, such as thylakoid membrane organization, the modulation of photosystem II (PS II) activity, carbon dioxide (CO2) assimilation, photorespiration, reactive oxygen species (ROS) scavenging, osmotic and ion homeostasis, abscisic acid (ABA) biosynthesis and signaling, and gene expression regulation, as well as protein synthesis and turnover. This review presents an overview of salt response in chloroplasts revealed by gene characterization efforts.

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“…It has been reported that salinity tolerance is closely related to antioxidant capacity in many plant species (Gossett et al 1994;Hernandez et al 1995;GuetaDahan et al 1997;Dionisio-Sese and Tobita 1998;Meneguzzo et al 1999;Hernandez et al 2000;Sreenivasulu et al 2000;Shalata et al 2001;Bor et al 2003;Mittova et al 2003;Turkan 2004, 2005;Koca et al 2006Koca et al , 2007Sekmen et al 2007;Yazici et al 2007;). In addition, transgenic plants overexpressing ROS-scavenging enzymes such as SOD (Badawi et al 2004;Tseng et al 2007), CAT (Tseng et al 2007), APX (Wang et al 1999) and GR (Foyer et al 1995) showed enhanced tolerance to the oxidative stresses in plants under adverse conditions.…”

Section: Oxidative Stress Induced By Salt Stressmentioning

confidence: 99%

Physiological adaptive mechanisms of plants grown in saline soil and implications for sustainable saline agriculture in coastal zone

et al. 2013

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There is large area of saline abandoned and lowyielding land distributed in coastal zone in the world. Soil salinity which inhibits plant growth and decreases crop yield is a serious and chronic problem for agricultural production. Improving plant salt tolerance is a feasible way to solve this problem. Plant physiological and biochemical responses under salinity stress become a hot issue at present, because it can provide insights into how plants may be modified to become more tolerant. It is generally known that the negative effects of soil salinity on plants are ascribed to ion toxicity, oxidative stress and osmotic stress, and great progress has been made in the study on molecular and physiological mechanisms of plant salinity tolerance in recent years. However, the present knowledge is not easily applied in the agronomy research under field environment. In this review, we simplified the physiological adaptive mechanisms in plants grown in saline soil and put forward a practical procedure for discerning physiological status and responses. In our opinion, this procedure consists of two steps. First, negative effects of salt stress are evaluated by the changes in biomass, crop yield and photosynthesis. Second, the underlying reasons are analyzed from osmotic regulation, antioxidant response and ion homeostasis. Photosynthesis is a good indicator of the harmful effects of saline soil on plants because of its close relation with crop yield and high sensitivity to environmental stress. Particularly, chlorophyll a fluorescence transient has been accepted as a reliable, sensitive and convenient tool in photosynthesis research in recent years, and it can facilitate and enrich photosynthetic research under field environment.

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Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco (Nicotiana tabacum) chloroplasts

Cited by 281 publications

References 47 publications

Genotypic difference in response of peroxidase and superoxide dismutase isozymes and activities to salt stress in barley

Genotypic difference in response of peroxidase and superoxide dismutase isozymes and activities to salt stress in barley

Salinity Response in Chloroplasts: Insights from Gene Characterization

Physiological adaptive mechanisms of plants grown in saline soil and implications for sustainable saline agriculture in coastal zone

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