Enhanced total flavonoid accumulation and alleviated growth inhibition of germinating soybeans by GABA under UV-B stress

Gu, Minglang; Yang, Jia; Tian, Xin; Fang, Weiming; Xu, Jianyun; Yin, Yi

doi:10.1039/d2ra00523a

Cited by 10 publications

(3 citation statements)

References 46 publications

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“…Furthermore, the exogenous GABA can pass through the cell membrane and enter the cell, stimulate hormone production, regulate growth and development, and further cause intracellular physiological and biochemical changes, thereby regulating polyphenols metabolism pathway gene expression to achieve the enrichment effect. Some studies have found that under UV-B stress, exogenous GABA can increase the activity of flavonoid synthase and the expression of related genes, inducing the accumulation of flavonoids [43] . Therefore, the increase in polyphenols content of mung beans can be attributed to the combined effect of GABA, ultrasonic, and germination treatments.…”

Section: Resultsmentioning

confidence: 99%

Effect of ultrasound combined with exogenous GABA treatment on polyphenolic metabolites and antioxidant activity of mung bean during germination

Wang

Gao

et al. 2023

Ultrasonics Sonochemistry

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Section: Resultsmentioning

confidence: 99%

Effect of ultrasound combined with exogenous GABA treatment on polyphenolic metabolites and antioxidant activity of mung bean during germination

Wang

Gao

et al. 2023

Ultrasonics Sonochemistry

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“…Therefore, we speculated that K, P, and Ca play a role in the metabolic pathway of isoflavones during the early growth stages, resulting in elevated levels of isoflavonoid metabolites (genistein 7-O-beta-D-glucoside and daidzein). The enrichment of isoflavones can improve the adaptability of plants to unfavorable environments, including low temperatures, UV radiation, and heavy-metal stress (Ma et al, 2018;Veremeichik et al, 2021;Gu et al, 2022). This suggests that the application of K and P fertilizers during the development of S. japonica can enhance its environmental adaptability (Liu et al, 2017).…”

Section: Changes In the Inorganic Elements Of Fs In Different Stagesmentioning

confidence: 99%

Combined analysis of inorganic elements and flavonoid metabolites reveals the relationship between flower quality and maturity of Sophora japonica L.

Wang,

Tan,

et al. 2023

Front. Plant Sci.

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Flos Sophorae (FS), or the dried flower buds of Sophora japonica L., is widely used as a food and medicinal material in China. The quality of S. japonica flowers varies with the developmental stages (S1–S5) of the plant. However, the relationship between FS quality and maturity remains unclear. Inductively coupled plasma optical emission spectrometry (ICP-OES) and ultra-high performance liquid chromatography coupled with electrospray ionization-triple quadrupole-linear ion trap mass spectrometry (UPLC-ESI-Q TRAP-MS/MS) were used to analyze inorganic elements and flavonoid metabolites, respectively. A combined analysis of the inorganic elements and flavonoid metabolites in FS was conducted to determine the patterns of FS quality formation. Sixteen inorganic elements and 173 flavonoid metabolites that accumulated at different developmental stages were identified. Notably, 54 flavonoid metabolites associated with the amelioration of major human diseases were identified, and Ca, P, K, Fe, and Cu were postulated to influence flavonoid metabolism and synthesis. This study offers a novel perspective and foundation for the further exploration of the rules governing the quality of plant materials.

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“…In the isoflavonoid pathway, isoliquiritigenin is generated by condensation of 4-coumarate with CoA and 3-molonyl-CoA and transformed into daidzein. Daidzein is reduced and hydroxylated to produce (3R)-2′-hydroxydihydrodaidzein, the precursor of glycinol [ 33 , 43 , 44 ]. Finally, glyceollins I, II, and III are produced through the condensation of (-)-glycinol and prenyl diphosphate.…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq Dissects Incomplete Activation of Phytoalexin Biosynthesis by the Soybean Transcription Factors GmMYB29A2 and GmNAC42-1

Lin

Monsalvo

et al. 2023

Plants

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Glyceollins, isoflavonoid-derived antimicrobial metabolites, are the major phytoalexins in soybean (Glycine max). They play essential roles in providing resistance to the soil-borne pathogen Phytophthora sojae and have unconventional anticancer and neuroprotective activities that render them desirable for pharmaceutical development. Our previous studies revealed that the transcription factors GmMYB29A2 and GmNAC42-1 have essential roles in activating glyceollin biosynthesis, yet each cannot activate the transcription of all biosynthesis genes in the absence of a pathogen elicitor treatment. Here, we report that co-overexpressing both transcription factors is also insufficient to activate glyceollin biosynthesis. To understand this insufficiency, we compared the transcriptome profiles of hairy roots overexpressing each transcription factor with glyceollin-synthesizing roots treated with wall glucan elicitor (WGE) from P. sojae. GmMYB29A2 upregulated most of the WGE-regulated genes that encode enzymatic steps spanning from primary metabolism to the last step of glyceollin biosynthesis. By contrast, GmNAC42-1 upregulated glyceollin biosynthesis genes only when overexpressed in the presence of WGE treatment. This is consistent with our recent discovery that, in the absence of WGE, GmNAC42-1 is bound by GmJAZ1 proteins that inhibit its transactivation activity. WGE, and not GmMYB29A2 or GmNAC42-1, upregulated the heat shock family gene GmHSF6-1, the homolog of Arabidopsis HSFB2a that directly activated the transcription of several glyceollin biosynthesis genes. Our results provide important insights into what biosynthesis genes will need to be upregulated to activate the entire glyceollin biosynthetic pathway.

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Enhanced total flavonoid accumulation and alleviated growth inhibition of germinating soybeans by GABA under UV-B stress

Abstract: Effect of GABA on isoflavone metabolism in soybeans under UV-B treatment.

Cited by 10 publications

References 46 publications

Effect of ultrasound combined with exogenous GABA treatment on polyphenolic metabolites and antioxidant activity of mung bean during germination

Effect of ultrasound combined with exogenous GABA treatment on polyphenolic metabolites and antioxidant activity of mung bean during germination

Combined analysis of inorganic elements and flavonoid metabolites reveals the relationship between flower quality and maturity of Sophora japonica L.

RNA-Seq Dissects Incomplete Activation of Phytoalexin Biosynthesis by the Soybean Transcription Factors GmMYB29A2 and GmNAC42-1

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