Enhanced Vascularization by Controlled Release of Platelet-Rich Plasma Impregnated in Biodegradable Gelatin Hydrogel

Kurita, Jiro; Miyamoto, Masaaki; Ishii, Yôsuke; Aoyama, Junya; Takagi, Gen; Naito, Zenya; Tabata, Yasuhiko; Ochi, Masami; Shimizu, Kazuo

doi:10.1016/j.athoracsur.2011.04.084

Cited by 65 publications

(58 citation statements)

References 29 publications

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“…This effect is due to PRP significantly reducing the invasion of inflammatory cells and increasing blood vessel density [131]. In addition, PRP treatment significantly increases the mRNA levels of VEGF and PDGF, which are associated with increased angiogenesis [131,200].…”

Section: Animal: Improvementmentioning

confidence: 99%

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

Kuffler

2015

Mol Neurobiol

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Platelet-rich plasma (PRP) has been tested in vitro, in animal models, and clinically for its efficacy in enhancing the rate of wound healing, reducing pain associated with injuries, and promoting axon regeneration. Although extensive data indicate that PRP-released factors induce these effects, the claims are often weakened because many studies were not rigorous or controlled, the data were limited, and other studies yielded contrary results. Critical to assessing whether PRP is effective are the large number of variables in these studies, including the method of PRP preparation, which influences the composition of PRP; type of application; type of wounds; target tissues; and diverse animal models and clinical studies. All these variables raise the question of whether one can anticipate consistent influences and raise the possibility that most of the results are correct under the circumstances where PRP was tested. This review examines evidence on the potential influences of PRP and whether PRP-released factors could induce the reported influences and concludes that the preponderance of evidence suggests that PRP has the capacity to induce all the claimed influences, although this position cannot be definitively argued. Well-defined and rigorously controlled studies of the potential influences of PRP are required in which PRP is isolated and applied using consistent techniques, protocols, and models. Finally, it is concluded that, because of the purported benefits of PRP administration and the lack of adverse events, further animal and clinical studies should be performed to explore the potential influences of PRP.

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Section: Animal: Improvementmentioning

confidence: 99%

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

Kuffler

2015

Mol Neurobiol

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“…Kurita et al demonstrated that PRP impregnated in biodegradable gelatin hydrogel can more effectively induce angiogenesis for critical ischemia treatment than PRP only. 54 Sell et al incorporated PRP into an electrospun scaffold of silk fibroin, polyglycolic acid, or polycaprolactone. 30 They found sustained release of growth factor proteins up to 35 days in culture.…”

Section: Future Directionsmentioning

confidence: 99%

Application of Platelet-Rich Plasma and Platelet-Rich Fibrin in Fat Grafting: Basic Science and Literature Review

Marra

2014

Tissue Engineering Part B: Reviews

131

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Due to the natural properties of fat, fat grafting remains a popular procedure for soft tissue volume augmentation and reconstruction. However, clinical outcome varies and is technique dependent. Platelet-rich plasma (PRP) contains a-granules, from which multiple growth factors such as platelet-derived growth factor, transforming growth factor-b, vascular endothelial growth factor, and epidermal growth factor can be released after activation. In recent years, the scope of PRP therapies has extended from bone regeneration, wound healing, and healing of musculoskeletal injuries, to enhancement of fat graft survival. In this review, we focus on the definition of PRP, the different PRP preparation and activation methods, and growth factor concentrations. In addition, we discuss possible mechanisms for the role of PRP in fat grafting by reviewing in vitro studies with adipose-derived stem cells, preadipocytes, and adipocytes, and preclinical and clinical research. We also review platelet-rich fibrin, a so-called second generation PRP, and its slow-releasing biology and effects on fat grafts compared to PRP in both animal and clinical research. Finally, we provide a general foundation on which to critically evaluate earlier studies, discuss the limitations of previous research, and direct plans for future experiments to improve the optimal effects of PRP in fat grafting.

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“…In this sense, platelet lysate (PL) offer great potential in regenerative medicine as an alternative source of growth factors (GFs) [5][6] . These PL-origin GFs, include fibroblast growth factor (FGF), vascular endothelial growth factor, platelet-derived growth factor, transforming growth factors-β1 and -β2, insulin-like growth factor, epidermal growth factor, epithelial cell growth factor, hepatocyte growth factor and bone morphogenetic proteins [7][8][9] are known to be involved in essential stages of wound healing and regenerative processes such as chemotaxis, cell proliferation and differentiation [10][11] . Moreover, platelets release numerous cell adhesion molecules (fibrin, fibronectin and vitronectin) which can provide a provisional matrix for the adhesion and migration of cells 10 .…”

mentioning

confidence: 99%

Platelet Lysate-Loaded Photocrosslinkable Hyaluronic Acid Hydrogels for Periodontal Endogenous Regenerative Technology

Babo

Pires

Santos

et al. 2017

ACS Biomater. Sci. Eng.

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The integrity and function of the periodontium can be compromised by traumatic injuries or periodontitis. Currently available clinical therapies are able to stop the progression of periodontitis and allow the healing of periodontal tissue. However an optimal strategy capable of restoring the anatomy and functionality of the lost periodontal tissue is still to be achieved. This injectable system is based on a photocrosslinkable hydrogel, prepared from methacrylated Hyaluronic Acid (me-HA) and incorporating Platelet Lysate (PL). The delivery of growth factors and cells in situ is expected to enhance regeneration of the periodontium. Various formulations of me-HA containing increasing PL concentrations were studied for achieving the formation of stable photocrosslinkable hydrogels. The produced hydrogels were subsequently characterized to assess mechanical properties, degradation, protein/growth factor release profile, antimicrobial activity and response towards human Periodontal Ligament fibroblasts (hPDLFs). The results demonstrated that it was possible to obtain stable photocrosslinkable hydrogels incorporating different amounts of PL that can be released in a sustained manner. Furthermore, the incorporation of PL improved (p<0.02) the viscoelastic properties of the hydrogels and enhanced their resilience to the degradation by hyaluronidase (HAase). Additionally, the PL showed to provide antimicrobial properties.Finally, hPDLFs, either seeded or encapsulated into the developed hydrogels, showed enhanced proliferation over time (p<0.05), proportionally to the increasing amounts of PL present in the hydrogel formulations.

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Enhanced Vascularization by Controlled Release of Platelet-Rich Plasma Impregnated in Biodegradable Gelatin Hydrogel

Cited by 65 publications

References 29 publications

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

Application of Platelet-Rich Plasma and Platelet-Rich Fibrin in Fat Grafting: Basic Science and Literature Review

Platelet Lysate-Loaded Photocrosslinkable Hyaluronic Acid Hydrogels for Periodontal Endogenous Regenerative Technology

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