“…The LPS concentrations in the plasma, milk, and rumen fluid were determined through a chromogenic end-point Tachypleus Amebocyte Lysate (TAL) assay kit (EC80545S, Chinese Horseshoe Crab Reagent Manufactory Co. Ltd, Xiamen, China) by referring to the manufacture's instruction and methods introduced precedently ( Tao et al., 2014 ; Wang et al., 2020a , Wang et al., 2020b ). In order to ensure that LPS concentrations were in the appropriate range, the dilution for those pretreated samples were carried out as previously described ( Wang et al., 2020a , Wang et al., 2020b ). The determinations for the LPS-related cytokines (Toll-like receptor [TLR]-4, interleukin [IL]-1β, IL-2, IL-6, and tumor necrosis factor alpha [TNF-α]), and LPS-related acute phase proteins (lipopolysaccharide binding protein [LBP], haptoglobin [Hp] and serum amyloid A [SAA]) in the serum of cattle were conducted in duplicates using corresponding enzyme-linked immunosorbent assay (ELISA) kits (CSB-EL023603BO, CSB-E09810B, CSB-E12899B, CSB-E08585B, CSB-E16370B, CSB-E08592B, CSB-E12020B, Cusabio Technology, Wuhan, China), as depicted by manufacturer's manuals and precedent investigations ( Wang et al., 2020a , Wang et al., 2020b ; Zhou et al., 2014 ).…”