Enhancing flora balance in the gastrointestinal tract of mice by lactic acid bacteria from Chinese sourdough and enzyme activities indicative of metabolism of protein, fat, and carbohydrate by the flora

Abstract

In this study, we investigated the effect of administration of 5 strains of lactic acid bacteria (LAB) isolated from traditional Chinese sourdough on the flora balance of gastrointestinal tract of mice. We specifically measured Enterococcus, Enterobacter, Bacteroides, and Lactobacillus by plate count and real-time PCR methods, and α-glucosidase, lactate dehydrogenase, esterase, and aminopeptidase activities as indicative of metabolism of sugar, fat, and protein from LAB isolated from feces of mice in vitro. The results showed that administration of Lactobacillus acidophilus LAC0201 and Lactobacillus fermentum LFE0302 lowered the uricacid index of serum. Lactobacillus acidophilus LAC0201, L. fermentum LFE0302, as well as Lactobacillus curvatus LCU0401 administration resulted in a reduction in the opportunistic pathogens (i.e., Enterococcus and Enterobacter), meanwhile, administration of L. fermentum LFE0302 and Lactobacillus sp. ULA0104 resulted in an increase in the counts of Lactobacillus. Lactobacillus fermentum LFE0302 administration increased starch digestion of intestinal flora after 4wk of feeding and also resulted in increased α-glucosidase activity in the intestinal flora after 3wk of feeding. We found a similar trend in esterase activity after administration of L. acidophilus LAC0201 for 3wk. Hence, our study suggested that LAB from Chinese sourdough might be used as potential probiotics to strengthen the flora balance in gastrointestinal tract and positively change the metabolism of nutrients through bacterial enzyme activities.

Limosilactobacillus fermentum JL-3 isolated from “Jiangshui” ameliorates hyperuricemia by degrading uric acid

“…The constructed model of the hyperuricemia mice efficiently elevated serum UA in mice more stably, compared with oteracil potassium or high purine diet alone to sustain a longer duration of hyperuricemia. 26,34,35 Thus, this method was adopted to construct hyperuricemia mice and gavaged with the strain (Figure 3b). Feces of mice were collected to investigate the survival of JL-3.…”

“…For inducting of UA-induced hyperuricemia, mice were given oteracil potassium (OP) and UA in 0.5 mL water for 14 d. Fifty mice were randomly divided into five groups, where OP and UA were used to construct the hyperuricemia animal model according to literature. 34,79 The control group was then fed with a basal diet, and orally gavaged with 0.5 mL of water, while the UA 1 group was given the basal diet along with OP (4%) and UA (1%) injection through the oral gavaged route. The UA 2 group was also given the basal diet of OP (4 %) and UA (2 %), which were injected through the oral gavaged route, while the UA 3 group was fed the basal diet and intraperitoneally injected with OP (4%) and UA (2%) in 0.5 mL water.…”

Genome-scale modeling and transcriptome analysis of Leuconostoc mesenteroides unravel the redox governed metabolic states in obligate heterofermentative lactic acid bacteria

“…They are also known to improve the microfloral balance in gastrointestinal tract; for example, Lb. fermentum is shown to strengthen it possibly through the increased activity of α-glycosidases and esterases 11 . In a different study, it is reported that a L. mesenteroides strain produces the prebiotic isomaltooligosaccharides that in turn enhance the growth of Bifidobacterium and Lactobacillus species in poultry chicken 12 .…”

Effects of mixed lactic acid bacteria on intestinal microbiota of mice infected with Staphylococcus aureus

“…Studies have shown that the proportion of the intestinal microbiota differs between healthy individuals and those who are not. The diversity of the intestinal microbiota in patients with chronic kidney disease is lower, and the LAB strains affect liver and kidney function by changing the composition of the microbiota [ 31 ]. We previously found that the structure of the intestinal flora of mice treated with mixed LAB was restored and the symptoms of S. aureus infection improved [ 10 ], indicating that intestinal health was associated with disease recovery.…”