Enhancing hypericin production of Hypericum perforatum cell suspension culture by ozone exposure

Abstract

Accumulation of secondary metabolites is one of the common reactions of plants to ozone exposure in nature. To investigate the effect of ozone on the production of desired compounds of plant cell cultures, we assayed hypericin production of Hypericum perforatum suspension cell cultures treated with different doses of ozone at different culture phases. The results show that hypericin contents of the cells treated with 60 to 180 nL L(-1) ozone are significantly higher than those of the control, showing that ozone exposure may stimulate hypericin synthesis. Hypericin production of the cells treated with ozone at exponential phase is higher than that of lag and stationary phase, which suggests that exponential phase cell cultures are more responsive to ozone exposure than lag and stationary phase cells. The highest hypericin production is obtained by the cells exposed to 90 nL L(-1) ozone at late exponential phase for 3 h, being about fourfold of the control.

Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum

“…Cell suspension culture was initiated once the stable cell lines were obtained on the solid MS medium. Given the potential for deriving useful secondary metabolites from plant resource, cell suspension cultures of H. perforatum were established to produce hypericin, naphtodianthrones, and phenylpropanoids [12,14,18]. In addition, some efforts have been made for the establishment of adventitious root cultures of H. perforatum for the production of flavonoids, phenolics, and xanthones [5,[15][16][17].…”

“…Plant-mediated green biomimetic synthesis of silver nanoparticle was considered a widely acceptable technology for rapid production of silver nanoparticles for successfully meeting the excessive need and current market demand and resulting in a reduction in the employment or generation of hazardous substances to human health and the environment [11]. Till now, a number of efforts have been made for the establishment of cell and/or adventitious root cultures of H. perforatum for the production of hypericins, phenolics, and xanthones [12][13][14][15]. The information on the factors affecting biomass and aforementioned metabolites of H. perforatum was also investigated through elicitation and culture optimization [1,[16][17][18].…”

A high-density transcript linkage map with 1,845 expressed genes positioned by microarray-based Single Feature Polymorphisms (SFP) in Eucalyptus

“…Nevertheless, this gene map not only offers a coarse first glance at the still undefined gene space in Eucalyptus , but also provides a useful resource for improving the assembly of the E. grandis genome, currently in progress [45,46]. In poplar, even a relatively modest 324 SFP map allowed localizing over 35 million base pairs of previously unplaced whole-genome shotgun (WGS) scaffold sequence to putative locations in the genome of Populus trichocarpa [24].…”

Abscisic acid plays critical role in ozone‐induced taxol production of Taxus chinensis suspension cell cultures

“…22,27 Although the impact of ozone on whole plants in nature has been well documented, the effect of ozone on the production of desired compounds in plant cell cultures has rarely been reported. 28 The results of this work show that treatment of ozone stimulates Taxol production of T. chinensis cells, which suggests that ozone might be a potential elicitor for improving Taxol production of plant cell cultures.…”