Enhancing lipid productivity by modulating lipid catabolism using the CRISPR-Cas9 system in Chlamydomonas

Nguyễn, Thu Hà; Park, Seunghye; Jeong, Jooyeon; Shin, Ye Sol; Sim, Sang Jun; Jin, EonSeon

doi:10.1007/s10811-020-02172-7

Cited by 42 publications

(11 citation statements)

References 44 publications

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“…In an investigation, a mutant strain of Thalassiosira pseudonana showed 3.5 times more lipid synthesis after inactivation of the specific multiuse enzymes acyltransferase/lipase/phospholipase [35]. In another study, Nguyen et al [36] reported that the suppression of the gene involved in FA degradation of Chlamydomonas mutant, Cre01.g 000300, could increase lipid content without impacting the growth rate.…”

Section: Lipid Biosynthesis Pathwaymentioning

confidence: 99%

“…The suppression of lipid catabolism, particularly the enzymes that catalyze the FA release, is another promising approach for increasing algal lipid productivity [35,88,89]. Lipid catabolism promotes membrane reconstruction by providing acyl groups, which is necessary for membrane reorganization of the photosynthetic system in microalgae [36,90]. However, some studies have indicated that, unlike the suppression of carbohydrate catabolism pathways, the inhibition of genes involved in lipid catabolism may have less influence on growth rate [35,89].…”

Section: Lipid Biosynthesis Pathwaymentioning

confidence: 99%

“…For instance, in an investigation, after inactivation of the specific multiuse enzymes acyltransferase/lipase/phospholipase, a mutant strain of Thalassiosira pseudonana showed 3.5 times more lipid content without decreasing growth rate [35]. In another study, Nguyen et al [36] reported that the suppression of the gene involved in fatty acid (FA) degradation of Chlamydomonas mutant, Cre01.g 000300, could increase lipid content without impacting the growth rate. Up to date, a number of review articles have been published on genetic engineering techniques for promoting the lipid productivity of algae species.…”

Section: Introductionmentioning

confidence: 99%

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Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Shokravi

Ong

et al. 2020

Sustainability

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Microalgae have received widespread interest owing to their potential in biofuel production. However, economical microalgal biomass production is conditioned by enhancing the lipid accumulation without decreasing growth rate or by increasing both simultaneously. While extensive investigation has been performed on promoting the economic feasibility of microalgal-based biofuel production that aims to increase the productivity of microalgae species, only a handful of them deal with increasing lipid productivity (based on lipid contents and growth rate) in the feedstock production process. The purpose of this review is to provide an overview of the recent advances and novel approaches in promoting lipid productivity (depends on biomass and lipid contents) in feedstock production from strain selection to after-harvesting stages. The current study comprises two parts. In the first part, bilateral improving biomass/lipid production will be investigated in upstream measures, including strain selection, genetic engineering, and cultivation stages. In the second part, the enhancement of lipid productivity will be discussed in the downstream measure included in the harvesting and after-harvesting stages. An integrated approach involving the strategies for increasing lipid productivity in up- and down-stream measures can be a breakthrough approach that would promote the commercialization of market-driven microalgae-derived biofuel production.

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Section: Lipid Biosynthesis Pathwaymentioning

confidence: 99%

Section: Lipid Biosynthesis Pathwaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Shokravi

Ong

et al. 2020

Sustainability

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“…The different result in the study by Li et al, found that the content of C16:0 in total lipid decrease while the content of C20:5 increase in OmTGL mutant in P. tricornutum compared to wild type. In addition, KD of phospholipase A2 in Chlamydomonas reinhardtii shows decrease in fatty acid composition of C16 and C18:3 compared to wild type 22 , 23 . Different result in our study most probably causes by different substrate specificity of different lipase.…”

Section: Resultsmentioning

confidence: 92%

Assessment on the oil accumulation by knockdown of triacylglycerol lipase in the oleaginous diatom Fistulifera solaris

Maeda

Watanabe

Kaha

et al. 2021

Sci Rep

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Microalgae are promising producers of biofuel due to higher accumulation of triacylglycerol (TAG). However, further improvement of the lipid metabolism is critical for feasible application of microalgae in industrial production of biofuel. Suppression of lipid degradation pathways is a promising way to remarkably increase the lipid production in model diatoms. In this study, we established an antisense-based knockdown (KD) technique in the marine oleaginous diatom, Fistulifera solaris. This species has a capability to accumulate high content of lipids. Tgl1 KD showed positive impact on cell growth and lipid accumulation in conventional culture in f/2 medium, resulting in higher oil contents compared to wild type strain. However, these impacts of Tgl1 KD were slight when the cells were subjected to the two-stage growth system. The Tgl1 KD resulted in slight change of fatty acid composition; increasing in C14:0, C16:0 and C16:1, and decreasing in C20:5. This study indicates that, although Tgl1 played a certain role in lipid degradation in F. solaris, suppression of only a single type of TAG lipase was not significantly effective to improve the lipid production. Comprehensive understanding of the lipid catabolism in this microalga is essential to further improve the lipid production.

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“…For example, co-overexpression of two endogenous key enzymes responsible for TAG biosynthesis, plastidial lysophosphatidic acid acyltransferase (LPAAT1) and diacylglycerol acyltransferase 2 (DGAT2), increased neutral lipid contents in Neochloris oleoabundans twofold ( Chungjatupornchai and Fa-Aroonsawat, 2020 ). Meanwhile, the knockout or silencing of genes involved in lipid biosynthesis could also improve the yields of natural edible oil and biofuel production ( Shin et al, 2018 ; Nguyen et al, 2020 ). Nevertheless, the increasing trend of improving TAG synthesis by using genetic engineering techniques were lower than that caused by certain nutrient or element deficiencies.…”

Section: Introductionmentioning

confidence: 99%

A U-Box Type E3 Ubiquitin Ligase Prp19-Like Protein Negatively Regulates Lipid Accumulation and Cell Size in Chlamydomonas reinhardtii

et al. 2022

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Microalgae lipid triacylglycerol is considered as a promising feedstock for national production of biofuels. A hotspot issue in the biodiesel study is to increase TAG content and productivity of microalgae. Precursor RNA processing protein (Prp19), which is the core component of eukaryotic RNA splice NTC (nineteen associated complex), plays important roles in the mRNA maturation process in eukaryotic cells, has a variety of functions in cell development, and is even directly involved in the biosynthesis of oil bodies in mouse. Nevertheless, its function in Chlamydomonas reinhardtii remains unknown. Here, transcriptional level of CrPrp19 under nutrition deprivation was analyzed, and both its RNA interference and overexpressed transformants were constructed. The expression level of CrPrp19 was suppressed by nitrogen or sulfur deficiency. Cell densities of CrPrp19 RNAi lines decreased, and their neutral lipid contents increased 1.33 and 1.34 times over those of controls. The cells of CrPrp19 RNAi lines were larger and more resistant to sodium acetate than control. Considerably none of the alterations in growth or neutral lipid contents was found in the CrPrp19 overexpression transformants than wild type. Fatty acids were also significantly increased in CrPrp19 RNAi transformants. Subcellular localization and yeast two-hybrid analysis showed that CrPrp19 was a nuclear protein, which might be involved in cell cycle regulation. In conclusion, CrPrp19 protein was necessary for negatively regulating lipid enrichment and cell size, but not stimulatory for lipid storage.

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Enhancing lipid productivity by modulating lipid catabolism using the CRISPR-Cas9 system in Chlamydomonas

Cited by 42 publications

References 44 publications

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Assessment on the oil accumulation by knockdown of triacylglycerol lipase in the oleaginous diatom Fistulifera solaris

A U-Box Type E3 Ubiquitin Ligase Prp19-Like Protein Negatively Regulates Lipid Accumulation and Cell Size in Chlamydomonas reinhardtii

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