Enhancing polyhydroxybutyrate production from high cell density fed-batch fermentation of Bacillus megaterium BA-019

Kanjanachumpol, Pawut; Kulpreecha, Songsri; Tolieng, Vasana; Thongchul, Nuttha

doi:10.1007/s00449-013-0885-7

Cited by 50 publications

(27 citation statements)

References 29 publications

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“…P(3HB) is produced via two phases. The first phase, named the “inoculum phase” or “cell growth”, occurs in a complex culture medium, usually nutrient broth (NB) [11–13], nutrient rich (NR) [14], basal culture medium (BCM) [15–16] or yeast malt (YM) [17] compounds of peptone and yeast, malt and meat extracts and complemented with a low C/N ratio. The second phase, named “polymer accumulation or production” occurs in mineral salt medium (MSM) [11–16,18–19] under the limitation of essential nutrients N, P, O, or Mg and in the presence of excess carbon sources associated with a high C/N ratio [11].…”

Section: Introductionmentioning

confidence: 99%

Complete factorial design to adjust pH and sugar concentrations in the inoculum phase of Ralstonia solanacearum to optimize P(3HB) production

et al. 2017

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Poly(3-hydroxybutyrate) (P(3HB)) is a biodegradable plastic biopolymer that accumulates as lipophilic inclusions in the cytoplasm of some microorganisms. The biotechnological process by which P(3HB) is synthesized occurs in two phases. The first phase involves cell growth in a complex culture medium, while the second phase involves polymer accumulation in the presence of excess carbon sources. As such, the efficiency of the second phase depends on the first phase. The aim of this study was to evaluate culture media with different concentrations of sucrose and glucose and different pH values in the inoculum phase of Ralstonia solanacearum RS with the intention of identifying methods by which the biomass yield could be increased, subsequently enhancing the yield of P(3HB). The culture medium was formulated according to the experimental planning type of central composite rotational design 22. The independent variables were pH and sugar concentration (sucrose and glucose), and the dependent variables were OD600nm, dry cell weight (DCW), and P(3HB) yield. The highest cell growth, estimated by the OD600nm (20.6) and DCW (5.35) values, was obtained when sucrose was used in the culture medium at a concentration above 35 g.L-1 in combination with an acidic pH. High polymer (45%) accumulation was also achieved under these conditions. Using glucose, the best results for OD600nm (12.5) and DCW (2.74) were also obtained at acidic pH but with a sugar concentration at the minimum values evaluated. Due to the significant accumulation of polymer in the cells that were still in the growth phase, the accumulating microorganism P(3HB) Ralstonia solanacearum RS can be classified as having type II metabolism in relation to the polymer accumulation phase, which is different from other Ralstonia spp. studied until this time.

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Section: Introductionmentioning

confidence: 99%

Complete factorial design to adjust pH and sugar concentrations in the inoculum phase of Ralstonia solanacearum to optimize P(3HB) production

et al. 2017

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“…However, the cell biomass and P(3HB) production recorded by Nuzi and co-workers (2012) was lower as compared with other Bacillus spp. used in other studies (Kulpreecha et al, 2009;Kanjanachumpol et al 2013). Therefore, in this study, higher C/N ratios were tested using B. megaterium UMTKB-1 strain to observe their effects on cell growth and P(3HB) accumulation.…”

Section: Effect Of Different C/n Ratios On P(3hb) Productionmentioning

confidence: 99%

“…(Kanjanachumpol et al, 2013;Kulpreecha et al, 2009). A high sugar content in cane molasses supports cell growth as well as PHA accumulation (Du et al, 2012).…”

Section: Effect Of Different C/n Ratios On P(3hb) Productionmentioning

confidence: 99%

“…Nevertheless, PHA produced by Gram-positive bacteria lacks lipopolysaccharide (LPS) endotoxins, and thus provides a wider application in medical such as blood carrier as well as bone plates and pharmaceutical fields such as drug delivery system (Reddy et al, 2003). The Gram-positive Bacillus genus is known to produce PHA from different carbon sources such as glycerol, glucose, fructose, and sucrose (Kanjanachumpol et al, 2013;Nuzi et al, 2012). Although Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

“…are known to produce PHA from a combination of industrial wastes or byproducts (Kanjanachumpol et al, 2013). At present, commercially available PHA are industrially produced using microbial cultures, and total cost of their production is very high.…”

Section: Introductionmentioning

confidence: 99%

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Bioconversion of novel and renewable agro-industry by-products into a biodegradable poly(3-hydroxybutyrate) by marine Bacillus megaterium UMTKB-1 strain

Yatim¹,

Syafiq²,

Huong³

et al. 2017

bta

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Agro-industry by-products are abundant in various valuable compounds. Some of these raw materials are considered as a cheaper carbon source for polyhydroxyalkanoate (PHA) production when compared with pure substrates. It is however often a costly affair for industries to recover the residual carbon components. In this study, poly(3-hydroxybutyrate) [P(3HB)] was produced using a marine Bacillus megaterium UMTKB-1 strain from sweetwater, a by-product from cane sugar refining process. The bioconversion was initiated in shaken-flasks and fermenter experiments. Applications of seawater and sweetwater mixture as well as sweetwater only as PHA culture media were investigated. The produced polymer was characterized using Gas Chromatography (GC) and Gel Permeation Chromatography (GPC). P(3HB) accumulation from tested carbon sources ranged from 3 to 49 wt%. The strain could accumulate P(3HB) in saline conditions when minimal salts medium was replaced with seawater and sweetwater. The P(3HB) content was between 7 and 27 wt%. This strain was also able to grow and accumulate up to 14 wt% P(3HB) when sweetwater was the sole PHA biosynthesis medium. The weights of P(3HB) produced was in the range 3-12 × 10 5 with polydispersity index values ranging from 2.7 to 3.8. The agroindustry by-products have proven to be potential carbon feedstocks for P(3HB) production. The tested strain was able to grow and accumulate P(3HB) in a novel carbon substrate medium, the sweetwater. This by-product could be used as a raw material for P(3HB) production without any pretreatment.

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Current status and future perspectives on the biological production of polyhydroxyalkanoates

Che

Jin

Zhou

et al. 2023

Asia-Pacific J Chem Eng

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Nowadays, the environmental pollution caused by conventional petroleum‐based plastics is one of the most serious problems facing mankind, and it is of great significance to look for alternative plastics with biodegradable feature. Notably, the bioplastics based on polyhydroxyalkanoates (PHA) have been a focus on research and development during the past several decades. In this review, the recent advances on the biological production of PHA were summarized, mainly including biosynthetic pathways and methods, followed by the extraction processes. Furthermore, the current status on PHA production using industrial waste streams was also discussed. For the moment, the relative higher production cost hindered the large‐scale biological production of PHA. Therefore, some insightful strategies were presented about the future research and scale production on biological production of PHA.

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Enhancing polyhydroxybutyrate production from high cell density fed-batch fermentation of Bacillus megaterium BA-019

Cited by 50 publications

References 29 publications

Complete factorial design to adjust pH and sugar concentrations in the inoculum phase of Ralstonia solanacearum to optimize P(3HB) production

Complete factorial design to adjust pH and sugar concentrations in the inoculum phase of Ralstonia solanacearum to optimize P(3HB) production

Bioconversion of novel and renewable agro-industry by-products into a biodegradable poly(3-hydroxybutyrate) by marine Bacillus megaterium UMTKB-1 strain

Current status and future perspectives on the biological production of polyhydroxyalkanoates

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