Enhancing somatic embryogenesis in avocado (Persea americana Mill.) using a two-step culture system and including glutamine in the culture medium

Encina, Carlos López; Parisi, Ann Margaret; O’Brien, Chris; Mitter, Neena

doi:10.1016/j.scienta.2013.10.019

Cited by 18 publications

(19 citation statements)

References 20 publications

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“…This trait can be exploited for germplasm preservation if regeneration from callus could be achieved. Under the influence of plant growth regulators, cells can be induced to form pro-embryos or somatic embryos which can then be developed into intact plants [47], which has been possible with immature zygotic embryo tissues of avocado [48].…”

Section: Regeneration From Callusmentioning

confidence: 99%

Micropropagation of Avocado (<i>Persea Americana</i> Mill.)

Hiti-Bandaralage¹,

Hayward²,

Mitter³

2017

AJPS

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Avocado is a high demand, high value tropical fruit recognised for its nutritional value. Being planted as a grafted tree, propagation of avocado refers to propagation of rootstock cultivar, then graft it with bud-wood from a mature scion cultivar. Elite cultivar propagation is critical to maintain the quality of fruit and farm management practices. Avocado propagation through seeds exhibit high genetic variation, hence less appealing for orchard plantings. Rooting of cuttings is only possible through a complex, lengthy and expensive process called "Frolich and Platt method". This creates limitations on rapid industry expansion due to scarcity and high price of plants in many countries. Alternative propagation methods are sought over 5 decades. Potential of micropropagation has been well demonstrated for wide variety of economically important plants. Commercial application of micropropagation for avocado will undoubtedly boost the industry around the globe. In this review, we present the developments in micropropagation of recalcitrant species, avocado, over the last 45 years. We summarise the culture media composition, hormones, growth conditions for different stages of avocado micropropagation pipeline, elaborating on cultivar specificity for in vitro success, and problems encountered under in vitro conditions and during acclimatisation. Overview of the current knowledge is critical to focus on important aspects in protocol optimisation, to develop an efficient and effective micropropagation system for avocado as well as other woody plant species recalcitrant for micropropagation.

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Section: Regeneration From Callusmentioning

confidence: 99%

Micropropagation of Avocado (<i>Persea Americana</i> Mill.)

Hiti-Bandaralage¹,

Hayward²,

Mitter³

2017

AJPS

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“…l -Glutamine is an amino acid known for promoting and maintaining cell function (Newsholme et al 2003). It has been used to promote somatic embryo proliferation, maturation and germination of many plant species, including date palm (Encina et al 2014; Kim and Moon 2007; Zouine and El Hadrami 2007; Zouine et al 2005). Myo-inositol is a vitamin that promotes cell and protoplast division (Bellini et al 1990; Kim et al 2003; Kiviharju et al 2005), as well as callus growth (Sepehr and Ghorbanli 2002).…”

Section: Introductionmentioning

confidence: 99%

Optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. Mejhoul

et al. 2016

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The effects of major mineral salts, l-glutamine, myo-inositol and carbon source on shoot bud proliferation of date palm (Phoenix dactylifera L.) cv. Mejhoul were evaluated. Different concentrations of ammonium nitrate (NH4NO3; 550, 825 or 1650 mg/L), potassium nitrate (KNO3; 633.3, 950 or 1900 mg/L), calcium chloride dehydrate (CaCl2·2H2O; 147, 220 or 440 mg/L), potassium dihydrogen phosphate (KH2PO4; 57, 85 or 170 mg/L), magnesium sulfate heptahydrate (MgSO4·7H2O; 123, 185 or 370 mg/L), l-glutamine and myo-inositol (0.5, 1, 1.5 and 2 g/L), sucrose, sorbitol, mannitol or commercial granulated sugar (10, 20, 30, 40 or 50 g/L) were tested. The highest number of shoot buds per explant (18.7) occurred on the medium containing 825 mg/L NH4NO3, 1900 mg/L KNO3, 220 mg/L CaCl2·2H2O, 170 mg/L KH2PO4, 370 mg/L MgSO4·7H2O as well as 1 g/L l-glutamine, 2 g/L myo-inositol and 30 g/L sucrose. The results showed that the frequency of hyperhydricity significantly increased in media containing 1650 mg/L NH4NO3. The concentrations of l-glutamine, myo-inositol and carbon source significantly affected the number of shoot buds per explant. However, they had no effect on hyperhydricity, tissue browning and precocious rooting. Shoots of 4.5–6.0 cm in length were isolated and transferred onto hormone-free media for elongation and rooting. After 3 months, the developed plantlets were successfully transplanted in a glasshouse and over 90 % survived acclimatization.

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“…However, avocado production is threatened by different agents, mainly fungal phytopathogens, including Colletotrichum species, Phytophthora cinnamomi , and Euwallacea kuroshio [ 1 ]. The losses caused by these diseases run into millions of dollars and severely affect the health of the trees, production, and international trade of avocado [ 2 ]. There have been large-scale efforts to breed disease-resistant avocados [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Although somatic embryogenesis (SE) has been studied for several decades and considerable effort has been invested in establishing an efficient in vitro propagation system for avocado, the efficiency of somatic embryo conversion into plants remains low to non-existent [ 2 , 6 , 7 , 8 ]. It has been assumed that this is primarily due to a failure of somatic embryos to mature [ 6 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Phenylpropanoids Are Connected to Cell Wall Fortification and Stress Tolerance in Avocado Somatic Embryogenesis

Olivares-García

Mata-Rosas

Peña-Montes

et al. 2020

IJMS

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Somatic embryogenesis (SE) is a valuable model for understanding the mechanism of plant embryogenesis and a tool for the mass production of plants. However, establishing SE in avocado has been complicated due to the very low efficiency of embryo induction and plant regeneration. To understand the molecular foundation of the SE induction and development in avocado, we compared embryogenic (EC) and non-embryogenic (NEC) cultures of two avocado varieties using proteomic and metabolomic approaches. Although Criollo and Hass EC exhibited similarities in the proteome and metabolome profile, in general, we observed a more active phenylpropanoid pathway in EC than NEC. This pathway is associated with the tolerance of stress responses, probably through the reinforcement of the cell wall and flavonoid production. We could corroborate that particular polyphenolics compounds, including p-coumaric acid and t-ferulic acid, stimulated the production of somatic embryos in avocado. Exogen phenolic compounds were associated with the modification of the content of endogenous polyphenolic and the induction of the production of the putative auxin-a, adenosine, cellulose and 1,26-hexacosanediol-diferulate. We suggest that in EC of avocado, there is an enhanced phenylpropanoid metabolism for the production of the building blocks of lignin and flavonoid compounds having a role in cell wall reinforcement for tolerating stress response. Data are available at ProteomeXchange with the identifier PXD019705.

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Enhancing somatic embryogenesis in avocado (Persea americana Mill.) using a two-step culture system and including glutamine in the culture medium

Cited by 18 publications

References 20 publications

Micropropagation of Avocado (<i>Persea Americana</i> Mill.)

Micropropagation of Avocado (<i>Persea Americana</i> Mill.)

Optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. Mejhoul

Phenylpropanoids Are Connected to Cell Wall Fortification and Stress Tolerance in Avocado Somatic Embryogenesis

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Enhancing somatic embryogenesis in avocado (Persea americana Mill.) using a two-step culture system and including glutamine in the culture medium

Cited by 18 publications

References 20 publications

Micropropagation of Avocado (&lt;i&gt;Persea Americana&lt;/i&gt; Mill.)

Micropropagation of Avocado (&lt;i&gt;Persea Americana&lt;/i&gt; Mill.)

Optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. Mejhoul

Phenylpropanoids Are Connected to Cell Wall Fortification and Stress Tolerance in Avocado Somatic Embryogenesis

Contact Info

Product

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About

Micropropagation of Avocado (<i>Persea Americana</i> Mill.)

Micropropagation of Avocado (<i>Persea Americana</i> Mill.)