Enhancing Stability and Purity of Crude Chitinase of Achatina fulica by Crystallization

Metagenomic DNA Library: Exploration of Novel Genes Encoding Glycoside Hydrolases

“…The opportunity to open access into the source of new microbial genes, especially unculturable microbes. It is estimated that unculturable microbes reach 99% of the population of microbes in the nature [2]. Metagenomics is the applications method of genomics technology to unculturable microbiota from its environment ( [6]; [7]) and reservoir genes (gut, gastrointestinal [8], rumen [9], etc) with making genomic libraries, followed by analyzing the genetic information contained therein.…”

“…Glycoside hydrolase are produced by bacteria, fungi, and also found in animals such as mollusks, sea shells, termites, sea urchins, and blue mussel [1]. Achatina fulica is a species of mollusk that feeds on plants as a source of nutrients, including producing digestive gland to degrade food sources [2]. There are epithelial and microbiota cells in digestive gland (gantrointestinal) Achatina fulica that produce glycosides hydrolase enzyme [3].…”

Analysis of Molecular Modeling and Molecular Docking of Beta-glucanase from Metagenomic Expression Library as Candida Antibiofilm Candidate

“…2 Candida albicans pathogen fungi can cause a variety of diseases such as thrush, skin lesions, vulvovaginitis candidiasis, and gastrointestinal candidiasis. 3 The condition of Candida overgrowth tends to produce high metabolites, possibly harming the host body as it usually leads to the emersion of such degenerative diseases as Alzheimer's, Parkinson's, T2D, amyloidosis, cystic fibrosis, and crescent anemia. 4 Treatment of candidiasis has been given through antifungal.…”