Enhancing the accuracy of measurement of small molecule organic biomarkers

Teo, Tang Lin; Lippa, Katrice A.; Mackay, Lindsey G.; Yong, Sharon; Liu, Qinde; Camara, Johanna E.; Delatour, Vincent; Lee, Tong Kooi; Lalere, Béatrice; Connor, Gavin O’; Henrion, André; Kato, Megumi; Numata, Masahiko; Kwon, Ha-Jeong; Jeong, Ji‐Seon; Xu, Bin; Song, Da; Nammoonnoy, Jintana; Wollinger, Wagner

doi:10.1007/s00216-019-02153-x

Cited by 8 publications

(2 citation statements)

References 31 publications

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“…The development of detection methods for LMW compounds has implications for a range of sectors. Steroid and thyroid hormones are examples of LMW compounds monitored within clinical diagnostics, while toxic compounds, such as saxitoxin, are considered as chemical weapons and are of interest to security sectors [1,2]. In the food safety and environmental sectors, LMW compounds include natural toxins such as marine, freshwater, plant, and mycotoxins, as well as anthropogenic compounds, including pesticides and veterinary drug residues.…”

Section: The Application Of Anti-idiotypes Within Low Molecular Weigh...mentioning

confidence: 99%

A new chapter for anti-idiotypes in low molecular weight compound immunoassays

Mills

Campbell

2022

Trends in Biotechnology

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Section: The Application Of Anti-idiotypes Within Low Molecular Weigh...mentioning

confidence: 99%

A new chapter for anti-idiotypes in low molecular weight compound immunoassays

Mills

Campbell

2022

Trends in Biotechnology

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“…They can be used as excellent molecular probes to study physiological processes, and serve as biomarkers that can provide an important insight into the metabolic activities associated with pathological conditions and therapeutic treatments. [1][2][3][4] Identification and study of DNA-and protein-binding small molecules are also of paramount importance in genomics and the discovery of molecular medicines. [5][6] Despite their pivotal importance, it remains a significant challenge to detect small molecules given their small size (~1 nm), as compared to biomacromolecules.…”

Section: Introductionmentioning

confidence: 99%

A Dual‐Modal Single‐Antibody Plasmonic Spectro‐Immunoassay for Detection of Small Molecules

Zheng

Raj

et al. 2022

Small

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Small molecules play a pivotal role in regulating physiological processes and serve as biomarkers to uncover pathological conditions and the effects of therapeutic treatments. However, it remains a significant challenge to detect small molecules given the size as compared to macromolecules. Recently, the newly emerging plasmonic immunoassays based on surface‐enhanced Raman scattering (SERS) offer great promise to deliver extraordinary sensitivity. Nevertheless, they are limited by the intrinsic SERS intensity fluctuations associated with the SERS uncertainty principle. The single transducer that relies on the intensity change is also prone to false signals. Additionally, the prevailing sandwich immunoassay format proves less effective towards detecting small molecules. To circumvent these critical issues, a dual‐modal single‐antibody approach that synergizes both the intensity and shift of the peak‐based immunoassay with Raman enhancement, coined as the INSPIRE assay, is developed for small molecules detection. With two independent transduction mechanisms, it allows better prediction of analyte concentration and attenuation of signal artifacts, providing a new and robust strategy for molecular analysis. With a proof‐of‐concept demonstration for detection of free T4 and testosterone in serum matrix, the authors envision that the INSPIRE assay could be expanded for a wide spectrum of applications in biomedical diagnosis, discovery of new biopharmaceuticals, food safety, and environmental monitoring.

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