Enhancing the Productivity and Stability of Superoxide Dismutase from Saccharomyces cerevisiae TBRC657 and Its Application as a Free Radical Scavenger

Pinmanee, Phitsanu; Sompinit, Kamonwan; Arnthong, Jantima; Suwannarangsee, Surisa; Jantimaporn, Angkana; Khongkow, Mattaka; Nimchua, Thidarat; Sukyai, Prakit

doi:10.3390/fermentation8040169

Cited by 5 publications

(4 citation statements)

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“…SOD is an enzymatic antioxidant that reduces superoxide radicals to hydrogen peroxide and oxygen. SOD plays an essential protective role through superoxide dismutation (Pinmanee et al., 2022 ). The MDA in the training groups was significantly lower than in the ALI group.…”

Section: Discussionmentioning

confidence: 99%

Comparison of preventive and therapeutic effects of continuous exercise on acute lung injury induced with methotrexate

Rajizadeh,

Hosseini,

Bahrami

et al. 2023

Experimental Physiology

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Methotrexate (Mtx) is used to treat various diseases, including cancer, arthritis and other rheumatic diseases. However, it induces oxidative stress and pulmonary inflammation by stimulating production of reactive oxygen species and cytokines. Considering the positive effects of physical activity, our goal was to investigate the preventive and therapeutic role of continuous training (CT) on Mtx‐induced lung injury in rats. The rats were divided into five groups of 14 animals: a control group (C); a continuous exercise training group (CT; healthy rats that experienced CT); an acute lung injury with Mtx group (ALI); a pretreatment group with CT (the rats experienced CT before ALI induction), and a post‐treatment group with CT (the rats experienced CT after ALI induction). One dose of 20 mg/kg Mtx intraperitoneal was administered in the Mtx and training groups. Forty‐eight hours after the last exercise session all rats were sacrificed. According to our results, the levels of tumour necrosis factor‐α (TNF‐α), malondialdehyde (MDA), myeloperoxidase (MPO), GATA binding protein 3 (GATA3) and caspase‐3 in the ALI group significantly increased compared to the control group, and the levels of superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), interleukin‐10 (IL‐10), forkhead box protein 3 (FOXP3), and T‐bet decreased. In contrast, compared to the acute lung injury group, pretreatment and treatment with CT reduced TNF‐α, MDA, MPO, GATA3 and caspase‐3 and increased SOD, GPX, TAC, IL‐10, FOXP3 and T‐bet levels. The effects of CT pretreatment were more significant than the effects of CT post‐treatment. Continuous exercise training effectively reduced oxidative stress and inflammatory cytokines and ameliorated Mtx‐induced injury, and the effects of CT pretreatment were more significant than the effects of CT post‐treatment.New Findings What is the central question of this study? Considering the high prevalence of lung injury in society, does exercise as a non‐pharmacological intervention have ameliorating effects on lung injury? What is the main finding and its importance? Exercise can have healing effects on the lung after pulmonary injury through reducing inflammation, oxidative stress and apoptosis. Considering the lower side effects of exercise compared to drug treatments, the results of this study may be useful in the future.

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Section: Discussionmentioning

confidence: 99%

Comparison of preventive and therapeutic effects of continuous exercise on acute lung injury induced with methotrexate

Rajizadeh,

Hosseini,

Bahrami

et al. 2023

Experimental Physiology

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“…Total antioxidant capacity (T-AOC) represents the collective ability of antioxidant substances in the body to combat oxidative damage [ 37 ]. Superoxide Dismutase, known for its specialized physiological activity, plays a crucial role in scavenging free radicals within organisms.…”

Section: Discussionmentioning

confidence: 99%

Biphasic Fermentation of Trapa bispinosa Shells by Ganoderma sinense and Characterization of Its Polysaccharides and Alcoholic Extract and Analysis of Their Bioactivity

Sun,

Lei,

Chen

et al. 2024

Molecules

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Background: Trapa bispinosa shells (TBs) and its flesh (TBf) have been recognized for their medicinal properties, including antioxidant, antitumor, and immunomodulatory effects. Despite these benefits, TBs are often discarded as waste material, and their applications remain to be further explored. Methods: In this study, we optimized the solid-state fermentation process of Ganoderma sinense (GS) with TBs using a response surface experiment methodology to obtain the fermented production with the highest water extract rate and DPPH free radical scavenging activity. We prepared and characterized pre-fermentation purified polysaccharides (P1) and post-fermentation purified polysaccharides (P2). Alcoholic extracts before (AE1) and after (AE2) fermentation were analyzed for active components such as polyphenols and flavonoids using UPLC-QTOF-MS/MS (ultra-performance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry). Mouse macrophages (RAW 264.7) were employed to compare the immune-stimulating ability of polysaccharides and the antioxidant activity of AE1 and AE2. Results: Optimal fermentation conditions comprised a duration of 2 days, a temperature of 14 °C, and a humidity of 77%. The peak water extract yield and DPPH free radical scavenging rate of the water extract from TBs fermented by GS were observed under these conditions. The enhanced activity may be attributed to changes in the polysaccharide structure and the components of the alcoholic extract. The P2 treatment group indicated more secretion of RAW 264.7 cells of NO, iNOS, IL-2, IL-10, and TNF-α than P1, which shows that the polysaccharides demonstrated increased immune-stimulating ability, with their effect linked to the NF-кB pathway. Moreover, the results of the AE2 treatment group indicated that secretion of RAW 264.7 cells of T-AOC and T-SOD increased and MDA decreased, which shows that the alcoholic extract demonstrated enhanced antioxidant activity, with its effect linked to the Nrf2/Keap1-ARE pathway. Conclusions: Biphasic fermentation of Trapa bispinosa shells by Ganoderma sinense could change the composition and structure of the polysaccharides and the composition of the alcoholic extract, which could increase the products’ immunomodulatory and antioxidant activity.

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“…The strain S. cerevisiae TRBC657 and G. xylinus TISTR975 were deposited and can be accessed at Thailand Bioresources Research Center (TBRC) and Thailand Institute of Scientific and Technological Research (TISTR), Thailand, respectively. The culture media which used in this study were (1) YPD medium consisting of 1.0% (w/v) yeast extract, 2.0% (w/v) peptone and 2.0% (w/v) glucose; (2) SOD production medium consisting of 3.0% (w/v) yeast extract and 25.0% (w/v) molasses [34]; and (3) a coconut water-based medium for BC production consisting of 100.0 mL coconut water, 5.0 g sucrose and 2.5 g (NH 4 ) 2 SO 4 and the pH was adjusted to 4.5 with 5% (v/v) acetic acid [35].…”

Section: Microbial Isolates and Media Preparationmentioning

confidence: 99%

Purification and Immobilization of Superoxide Dismutase Obtained from Saccharomyces cerevisiae TBRC657 on Bacterial Cellulose and its Protective Effect against Oxidative Damage in Fibroblasts

et al. 2023

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Superoxide dismutase (SOD) is an essential enzyme that eliminates harmful reactive oxygen species (ROS) generating inside living cells. Due to its efficacities, SOD is widely applied in many applications. In this study, the purification of SOD produced from Saccharomyces cerevisiae TBRC657 was conducted to obtain the purified SOD that exhibited specific activity of 513.74 U/mg with a purification factor of 10.36-fold. The inhibitory test revealed that the purified SOD was classified as Mn-SOD with an estimated molecular weight of 25 kDa on SDS-PAGE. After investigating the biochemical characterization, the purified SOD exhibited optimal activity under conditions of pH 7.0 and 35 °C, which are suitable for various applications. The stability test showed that the purified SOD rapidly decreased in activity under high temperatures. To overcome this, SOD was successfully immobilized on bacterial cellulose (BC), resulting in enhanced stability under those conditions. The immobilized SOD was investigated for its ability to eliminate ROS in fibroblasts. The results indicated that the immobilized SOD released and retained its function to regulate the ROS level inside the cells. Thus, the immobilized SOD on BC could be a promising candidate for application in many industries that require antioxidant functionality under operating conditions.

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Enhancing the Productivity and Stability of Superoxide Dismutase from Saccharomyces cerevisiae TBRC657 and Its Application as a Free Radical Scavenger

Cited by 5 publications

References 50 publications

Comparison of preventive and therapeutic effects of continuous exercise on acute lung injury induced with methotrexate

Comparison of preventive and therapeutic effects of continuous exercise on acute lung injury induced with methotrexate

Biphasic Fermentation of Trapa bispinosa Shells by Ganoderma sinense and Characterization of Its Polysaccharides and Alcoholic Extract and Analysis of Their Bioactivity

Purification and Immobilization of Superoxide Dismutase Obtained from Saccharomyces cerevisiae TBRC657 on Bacterial Cellulose and its Protective Effect against Oxidative Damage in Fibroblasts

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