Enhancing the Stereoselectivity and Activity of Candida Species Lipase in Organic Solvent by Noncovalent Enzyme Modification^a

Directed Evolution of N‐Carbamyl‐d‐amino Acid Amidohydrolase for Simultaneous Improvement of Oxidative and Thermal Stability

“…Even supercritical fluids have been used as solvent, thus facilitating downstream processing (Marty et al 1992; Beckman et al 1995; Ikushima 1997). Intense research has been performed on the impact of various parameters on the stereoselectivity of lipases, such as immobilization, coating of lipases with surfactants, use of acid anhydrides as substrates, or controlling stereoselectivity via the surface pressure of substrate monolayers (Rogalska et al 1990, 1991, 1993a, 1993b; Ransac et al 1991; Bianchi et al 1993; Bornscheuer et al 1993, 1995; Cygler et al 1995; Kamiya et al 1995; Stadler et al 1995; Yang et al 1996; Koteshwar and Fadnavis 1997).…”

A model of the pressure dependence of the enantioselectivity of Candida rugosalipase towards (±)‐menthol

“…on the impact of various parameters on the stereoselectivity of lipases, such as immobilization, coating of lipases with surfactants, use of acid anhydrides as substrates, or controlling stereoselectivity via the surface pressure of substrate monolayers (Rogalska et al 1990(Rogalska et al , 1991(Rogalska et al , 1993a(Rogalska et al , 1993bRansac et al 1991;Bianchi et al 1993;Bornscheuer et al 1993Bornscheuer et al , 1995Cygler et al 1995;Kamiya et al 1995;Stadler et al 1995;Yang et al 1996;Koteshwar and Fadnavis 1997).…”