Enhancing thermal stability and lytic activity of phage lysin PlyAB1 from <i>Acinetobacter baumannii</i>

Yuan, Yingbo; Li, Qingbin; Zhang, Shuhang; Jin-hong, GU; Huang, Guangtao; Qi, Qingsheng; Lu, Xuemei

doi:10.1002/bit.28187

Cited by 5 publications

(2 citation statements)

References 44 publications

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“…Thermal stability was determined according to the method of Yuan et al 43 After the LysDZ25 and its variants were incubated at 37 °C, they were placed for 0, 5,10,15,20,25,30,35,40,55, and 60 min. Subsequently, the corresponding ΔOD 600nm value within 30 min was determined.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Identification and Molecular Modification of Staphylococcus aureus Bacteriophage Lysin LysDZ25

Chang

Zhang

et al. 2023

ACS Infect. Dis.

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With the continuous emergence and spread of drugresistant and multi-drug-resistant Staphylococcus aureus, traditional antibiotic treatment has gradually lost its effect. There is an urgent need to develop and study new and effective bio-green inhibitors to control S. aureus. In this study, the S. aureus phage DZ25 was isolated from milk and the lysin LysDZ25 with excellent tolerance to serum and NaCl solution was identified. Subsequently, to improve the lytic activity and thermal stability of LysDZ25, RoseTTAFold was used to construct threedimensional (3D) structures, molecular dynamics (MD) simulation was used for conformational acquisition, and the MDL strategy previously developed in our lab was used to rationally design variants. After two rounds of rational design, the optimal variant with improved thermal stability, S333V/N245R/D299L, was obtained, and its half-life time was 4.0-fold that of wild-type LysDZ25. At 37, 40, 45, and 50 °C, the lytic activity of the optimal triple-point variant S333V/N245R/ D299L was increased by 17.3-, 26.7-, 20.2-, and 50.1-fold compared with that of the wild-type LysDZ25, respectively. Finally, cell count was used to evaluate the lytic activity, and the results showed that the optimal variant S333V/N245R/D299L could drop about 3.5 log 10 values compared with the control and about 2.6 log 10 values compared with the wild-type LysDZ25.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Identification and Molecular Modification of Staphylococcus aureus Bacteriophage Lysin LysDZ25

Chang

Zhang

et al. 2023

ACS Infect. Dis.

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“…The final count of released phage particles was 5.0×10 12 PFU/mL, indicating a burst size of approximately 241 virions per cell. This suggests that VB_PHOS_XC has a lytic activity and a relatively short replication cycle (Yuan et al, 2022).…”

Section: Morphological and Biological Properties Of Phage Vb_phos_xcmentioning

confidence: 96%

Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tide

et al. 2023

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Pseudoalteromonas is a ubiquitous and abundant genus of marine bacteria commonly associated with algae. In this study, a novel siphoviral-morphological bacteriophage, vB_PhoS_XC, was isolated from the coastal seawaters of Qingdao (China) during a bloom of the Ulva prolifera (U. prolifera) green tide. The morphology of this phage (icosahedron head 51 ± 1 nm in diameter; a tail length of 86 ± 1 nm) was characterized through transmission electron microscope. The biological properties of this virus showed a short latent period (45 minutes), a large burst size (241 virions per cell) and a relatively wide range of temperatures/pH level tolerance (-20°C to 45°C and pH 4 to pH 10, respectively). The vB_PhoS_XC has a 46,490-bp double-stranded DNA genome with a G+C content of 40.0%, and encodes 72 open reading frames (ORFs). Thirty-five of these ORFs were assigned into known functions based on BLAST-based algorithm against NR database of GenBank. In addition, eco-genomic analysis provides the evidence of vB_PhoS_XC accompanied by bloom of U. prolifera, and confirmed the high expression of two phosphatase-metabolism-related auxiliary metabolic genes (AMGs). This study provides new insights into the functional and ecological roles of the Pseudoalteromonas phage vB_PhoS_XC, shedding light on the virological study approach combined with traditional isolation and meta-omics data.

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Improving the thermostability of 42 kDa chitinase from Trichoderma asperellum SH16 expressed in Nicotiana benthamiana via site-directed mutagenesis

Tien,

Van Thanh,

et al. 2024

Plant Cell Tiss Organ Cult

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Enhancing thermal stability and lytic activity of phage lysin PlyAB1 from Acinetobacter baumannii

Cited by 5 publications

References 44 publications

Identification and Molecular Modification of Staphylococcus aureus Bacteriophage Lysin LysDZ25

Identification and Molecular Modification of Staphylococcus aureus Bacteriophage Lysin LysDZ25

Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tide

Improving the thermostability of 42 kDa chitinase from Trichoderma asperellum SH16 expressed in Nicotiana benthamiana via site-directed mutagenesis

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