Enlightenment on the aequorin-based platform for screening <i>Arabidopsis</i> stress sensory channels related to calcium signaling

Yu, Zhiming; Taylor, Jemma L.; He, Yue; Ni, Jun

doi:10.1080/15592324.2015.1057366

Cited by 2 publications

(2 citation statements)

References 44 publications

(46 reference statements)

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“…In the last 10 years, aequorin has been widely used as a Ca 2+ indicator in a great variety of research: for studying the neural basis for Drosophila behavior [ 44 , 45 , 46 , 47 , 48 , 49 ]; exploring brain activity and visualizing Ca 2+ signaling events that occur at the early stages of zebrafish development [ 44 , 50 , 51 , 52 , 53 , 54 ]; elucidating the Ca 2+ response to biotic or abiotic stress in different plants [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 ] and yeast [ 63 ]; as well as for protozoa studies [ 64 ].…”

Section: Analytical Application Of Ca 2+ -Regulmentioning

confidence: 99%

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Krasitskaya

Bashmakova

Frank

2020

IJMS

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: The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.

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Section: Analytical Application Of Ca 2+ -Regulmentioning

confidence: 99%

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Krasitskaya

Bashmakova

Frank

2020

IJMS

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“…Upon GPCR activation, these aequorin-fused signaling proteins initially localized in the cytosol, translocate to the PM due to the larger concentration of Ca 2+ at the inner leaflet of the PM compared to the cytosol [212,213]. In order for aequorin to be luminescent, it requires a hydrophobic prosthetic group, coelenterazine, which converts the apo-aequorin to active aequorin [214]. After Ca 2+ binds to the high affinity sites in aequorin, it oxidizes coelenterazine into coelenteramide with the production of CO 2 and emission of blue light measurable at ~470 nm.…”

Section: Luminescence-based Detection Of Gpcr-g Protein Signaling 71mentioning

confidence: 99%

Optical approaches for single-cell and subcellular analysis of GPCR–G protein signaling

et al. 2019

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G protein-coupled receptors (GPCRs), G proteins and their signaling associates, are major signal transducers that control a majority of cellular signaling and regulate key biological functions including immune, neurological, cardiovascular, and metabolic processes. These pathways occupy over one-third of drugs on the market, however, the current understanding of their function is limited and primarily derived from cell-destructive approaches providing an ensemble of static, multi-cell information about the status and composition of molecules. Spatiotemporal behavior of molecules-involved is crucial to understanding in vivo cell behaviors both in health and disease, and the advent of genetically encoded fluorescence proteins and small fluorophore-based biosensors has facilitated the mapping of dynamic signaling in cells with subcellular acuity. Since we and others have developed optogenetic methods to regulate GPCR-G protein signaling in single cells and subcellular regions using dedicated wavelengths, the desire to develop and adopt optogenetically-amenable assays to measure signaling has motivated us to take a broader look at the available optical tools and approaches compatible with measuring single-cell and subcellular GPCR-G protein signaling. Here we review such key optical approaches enabling the examination of GPCR, G protein, secondary messenger, and downstream molecules such as kinase and lipid signaling in living cells. The methods reviewed employ both fluorescence and bioluminescence detection. We not only further elaborate the underlying principles of these sensors, but we also discuss the experimental criteria and limitations to be considered during their use in single-cell and subcellular signal-mapping.

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Enlightenment on the aequorin-based platform for screening Arabidopsis stress sensory channels related to calcium signaling

Cited by 2 publications

References 44 publications

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Optical approaches for single-cell and subcellular analysis of GPCR–G protein signaling

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