Enriched environment housing improved the laying hen's resistance to transport stress via modulating the heat shock protective response and inflammation

Abstract

An enriched environment can promote adaptability of animals to cope with complex environments. A total of 18-week-old 216 laying hens were randomly divided into 2 groups; of which, one group was housed in conventional battery cages (CC, n = 36), and the others were housed in furnished cages (FC, n = 180). At the end of 64 wk of age, 24 chickens of each group were selected for 4-hour transport treatment. The spleen tissues of laying hens were collected before transportation ( BT ), immediately after transportation, and at 48 h after transportation to detect the expression of the heat shock protective response signaling pathway and inflammatory factors. Serum samples were collected to detect the content of immune cytokines. Transport stress decreased heat shock proteins ( HSP ; including Small HSP, HSP27, HSP40, HSP60, HS70, HSP90, HSP110) in the CC group ( P < 0.05), whereas there was no significant difference in the expression of HSP (except for Small HSP and HSP40) in the FC group ( P > 0.05) immediately after transportation. At 48 h after transportation, mRNA levels of HSP (except for Small HSP and HSP40) in the FC group were upregulated, which were higher than those at BT ( P < 0.05). The changes in HSP60, HSP70, and HSP90 protein levels had similar tendencies. The results showed that housing in furnished cages alleviated the inhibition of expression of HSP in the hens' spleen induced by transport stress. In addition, the hens housed in the FC group had lower expression levels of proinflammatory factors (nuclear transcription factor-kappa B, inducible nitric oxide synthase, cyclooxygenase-2, prostaglandin E synthase, inflammatory cytokines [IL-1β and IL-6], and tumor necrosis factor alpha) ( P < 0.05). We suggest that the enriched environment can reduce transport stress damage in laying hens and improve resistance to transport stress by regulating expression of heat shock response proteins and inflammatory cytokines.

Deltamethrin induces apoptosis in cerebrum neurons of quail via promoting endoplasmic reticulum stress and mitochondrial dysfunction

“…Food and water were obtained freely throughout the entire experiment. 26,27 After a week of adaptive feeding, four groups of quails were randomly assigned (n = 10 per group): control group (normal saline administered by gavage), low dose (15 mg/kg DLM administered by gavage), medium dose (30 mg/kg DLM administered by gavage), and high dose (45 mg/kg DLM administered by gavage). Treatment duration was 12 weeks.…”

TMT Quantitative Proteomics Analysis Reveals the Effects of Transport Stress on Iron Metabolism in the Liver of Chicken

“…The pre-slaughter transport process is an important integral part of poultry management [ 1 ]. Pre-slaughtering transport of market-age broilers from their geographically dispersed farms is an unavoidable common practice [ 2 ].…”

Impacts of Embryonic Thermal Programming on the Expression of Genes Involved in Foie gras Production in Mule Ducks

“…More surprisingly, we also measured a significant decrease in HSP40 (DNAJB12) at E27 in the TM group compared to control. Mostly up-regulated after heat stress ( Dong et al, 2006 ; Neal et al, 2006 ; Chen et al, 2018 ), down-regulation of that chaperone has, however, already been measured immediately after a transport stress in the spleen of hens ( Li et al, 2021 ). These data suggest that the type of stress, the targeted tissue, or the time of stress exposure may have a different impact on the expression of different HSP family members.…”