Enrichment and qualitative analysis of flavonoid glycosides from the aerial parts of Glycyrrhiza uralensis Fisch

Abstract

Our previous study found that the aerial parts of Chinese liquorice (Glycyrrhiza uralensis Fisch.) had pharmacological effects against chronic non-bacterial prostatitis in rats, however the pharmacologically active compounds remain unclear. Here, a method based on UPLC-Q-Exactive Orbitrap-MS was established to qualitatively analyse the flavonoid glycosides rich fraction extracted from the aerial part of G. uralensis Fisch., after pretreatment with n-butanol and enrichment using AB-8 macroporous resin. Using both positive and negative ion modes, 52 compounds were identified or tentatively characterised by comparison with standards and the literature: 40 flavonoids, 8 organic acids, 2 chromones, 1 coumarin, and 1 phenylethanoid glycoside.This study provides not only an approach to enrich flavonoid glycosides but also a methodology for quickly determining the relevant bioactive components in the aerial parts of G. uralensis Fisch.

Design and optimization of heat pump with infrared drying for Glycyrrhiza uralensis (Licorice) processing

“…The total phenol content was determined using the Folin–Ciocalteu (FC) method ( 31 ), with gallic acid as the standard substance. The absorbance value of the standard solution was measured at 760 nm, and the total phenol content of the sample was expressed in gallic acid equivalents (GAE) ( 32 ). Additionally, the total flavonoid content in the sample solution was determined and expressed as the rutin equivalent (RE) in mg/g (dry base).…”