Enrichment of total steroidal saponins from the extracts ofTrillium tschonoskiiMaxim by macroporous resin and the simultaneous determination of eight steroidal saponins in the final product by HPLC

Abstract

An effective and simple method was established for the separation and enrichment of steroidal saponins from Trillium tschonoskii Maxim. The adsorption and desorption properties of seven macroporous resins were investigated. Among the tested resins, AB-8 resin showed the best adsorption and desorption capacities. The adsorption of steroidal saponins on AB-8 at 25°C was quite consistent with both the Freundlich isotherm model and the pseudo-second-order kinetics model. By optimizing the dynamic adsorption and desorption parameters, the content of steroidal saponins increased from 5.20% in the crude extracts to 51.93% in the final product, with a recovery yield of 86.67%. Furthermore, by scale-up separation, the concentration and recovery of total steroidal saponins were 43.8 and 85.5%, respectively, which suggested that AB-8 resin had great industrial and pharmaceutical potential because of its high efficiency and cost-effectiveness. In addition, a high-performance liquid chromatography method for the simultaneous determination of eight steroidal saponins was established for the first time, which was employed to qualitatively and quantitatively analyze the final product. Based on the methodological validation results, the high-performance liquid chromatography method can be widely applied to the quality control of steroidal saponins from Trillium tschonoskii Maxim due to its excellent accuracy, stability, and repeatability.

Separation and evaluation of natural antileishmanial potential against Leishmania major and infuntum isolated from the Tunisia strains

“…As described in the introduction section, recently saponins have become a research hotspot due to their multiple and strong bioactivities, especially their cytotoxic activities on a series of tumor cell lines related to their chemical structure (Bouabdallah et al, 2015;Nath et al, 2016;Zhang et al, 2016;Zhou et al, 2017). However our result also confirmed that the n-butanolic extract (saponin fraction) exhibited the best inhibitory activity on both of the leishmania parasites tested (Table II).…”

Effects of steroidal saponins extract from Ophiopogon japonicus root ameliorates doxorubicin-induced chronic heart failure by inhibiting oxidative stress and inflammatory response

“…The eluate was collected, and then lyophilized with freeze-dryer (Zhou et al. 2017). Finally, the contents of total steroidal saponins were assayed using a diosgenin as reference by UV spectrophotometry.…”

Bioactive Steroids and Saponins of the Genus Trillium

“…Thus far, several steroidal saponins have been isolated and purified from the plants belonging to this genus and, at present, novel metabolites/phytochemicals from Trillium ssp. are investigating using the latest technologies [ 30 , 31 , 32 , 33 , 34 ]. The secondary metabolites/phytochemicals of species belonging to the genus Trillium are reported in Table 2 .…”