Enrichment procedures for the isolation of <i>Salmonella, Arizona, Edwardsiella</i> and <i>Shigella</i> from faeces

Iveson, J. B.

doi:10.1017/s0022172400022804

Cited by 25 publications

(14 citation statements)

References 14 publications

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“…We have previously evaluated several liquid media for enrichment (unpublished data). These were strontium hydrogen selenite and strontium chloride B enrichment broth (Iveson, 1973), trypticase soy broth and tetrathionate broth (Bailey and Scott, 1974), potassium tellurite and alkaline peptone water (Furniss, Lee and Donovan, 1978), and nutrient broth (Cowan, 1974). Nutrient broth was the most satisfactory and produced excellent enrichment after incubation of faecal samples for 24 h. In a large epidemiological study of children with and without diarrhoea we found that Aeromonas spp.…”

Section: Isolation Of Aeromonas 409mentioning

confidence: 99%

Media for Isolation of Aeromonas Spp. from Faeces

Robinson

Burke

Worthy

et al. 1984

Journal of Medical Microbiology

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SUMMARY. Five solid media were evaluated for isolation of Aeromonas spp. from faeces: desoxycholate citrate agar (DCA), MacConkey's agar (MAC), xylose-desoxycholate-citrate agar (XDCA), Rogol's medium (ROG), which contained ampicillin 20 mg/L and p-nitrophenyl-glycerine 25 mg/L as inhibitors, and blood agar (BA) with ampicillin 10 mg/L. False negative oxidase tests limited the usefulness of DCA and MAC and, although the use of XDCA avoided the problem of fermentation of lactose, some Aeromonas spp. failed to grow on XDCA or produced minute colonies unsuitable for oxidase tests. BA yielded the highest rate of isolation for Aeromonas spp. from 323 faecal samples-1 5.2% for all Aeromonas spp. and 9.3% for enterotoxigenic (ENT+) strains. This compares with 10.8% for all strains and 6.5% for ENT+ strains isolated on DCA, 7.1% for all strains and 4% for ENT+ strains on MAC and 4% for all strains and 1.5% for ENT+ strains on ROG. Blood agar with ampicillin is recommended for isolation of Aeromonas spp. from faeces.

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Section: Isolation Of Aeromonas 409mentioning

confidence: 99%

Media for Isolation of Aeromonas Spp. from Faeces

Robinson

Burke

Worthy

et al. 1984

Journal of Medical Microbiology

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“…agar, and inoculated into strontium chloride B enrichment broth (Iveson, 1971) incubated at 430 C. and strontium selenite A broth (Iveson, 1973) incubated at 370 C. The enrichment media were plated out at 24 and 48 hr. intervals on D.C. and modified B.S.…”

Section: Introductionmentioning

confidence: 99%

Salmonella javianainfection in an infant associated with a marsupial, the quokka,Setonix brachyurus, in Western Australia

Iveson¹,

Bradshaw

1973

J. Hyg.

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SUMMARYSalmonella javiana, a serotype rarely isolated in Australia, has been recovered from the faeces of a 14-month-old infant with symptoms of enteritis.The child had been closely associated with a marsupial species, the quokka, during a vacation on Rottnest Island in Western Australia, and S. javiana was isolated from faecal pellets from adult quokkas, and also from a snake collected on the island.Sampling revealed a high incidence of Salmonella infection in the quokkas. In all, 62 out of 87 animals (71 %) were found to be infected, and 17 Salmonella and 3 Arizona serotypes were identified from 100 isolations comprising 92 salmonellas and 8 arizonas. Multiple infections were frequently detected and up to four serotypes were recovered from individual animals. S. javiana was isolated from four quokkas.A close parallel was observed between the serotypes isolated from quokkas and sea-gulls on the island, and abattoir effluents, lake waters, bird droppings and reptiles sampled on the adjacent mainland.The epidemiological significance of Salmonella and Arizona infections in the quokka population and their possible association with the seasonal decline in condition and numbers of animals on Rottnest Island is discussed.

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“…is that protein which is carried over from the non-selective enrichment broth exerts a negative effect upon the selectivity of the bi-selenite ion (Leifson, 1936;North and Bartram, 1953). Incubation of the selective enrichment broths at 43° rather than at 37° may have reduced the selectivity of strontium bi-selenite, but this effect was not apparent in the results of Iveson (1973). The effect of alteration from 0-1% w/v potassium di-hydrogen phosphate in the formulation of S.S.B.…”

Section: Resultsmentioning

confidence: 95%

“…were prepared by the method of Iveson andMacKay-ScoUay (1969, 1972), and S.S.A. as described by Iveson (1973).…”

Section: Materials and Methocsmentioning

confidence: 99%

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An Evaluation of Selective Broths Based on the Bi‐selenite Ion and on Hypertonic Strontium Chloride in Salmonellae Detection in Egg Products

Ra¹

1976

Aust J Exp Biol Med

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Summary. Of the 104 isolations of Salmonella sp. from egg pulp, 97 were obtained from strontium chloride M broth, 42 from strontium selenite broth and 57 from strontium selenite A broth. The results suggest that the Hrst medium may be used more successfully than bi-selenite based media for enrichment and subsequent detection of salmonellae in egg products; however, the growth of S. piiHorum was not satisfactory in strontium chloride M broth.INTRODUCTION.

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Enrichment procedures for the isolation of Salmonella, Arizona, Edwardsiella and Shigella from faeces

Cited by 25 publications

References 14 publications

Media for Isolation of Aeromonas Spp. from Faeces

Media for Isolation of Aeromonas Spp. from Faeces

Salmonella javianainfection in an infant associated with a marsupial, the quokka,Setonix brachyurus, in Western Australia

An Evaluation of Selective Broths Based on the Bi‐selenite Ion and on Hypertonic Strontium Chloride in Salmonellae Detection in Egg Products

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