Enrichment technique for the selection of catabolite repression-resistant mutants of Trichoderma as producers of cellulase

Labudová, I.

doi:10.1016/0378-1097(83)90198-2

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…A number of specific selection schemes have been adapted to improve the biosynthetic capacity of production strains. Resistance to the toxic glucose analogue (2-deoxyglucose) has been used as a criterion to select mutants showing increased rates of glucoamylase (Fiedurek et al 1987) and cellulase (Labudova and Farkas 1983). High levels of chymosin derivative have been isolated from the culture of , -I .…”

Section: Resultsmentioning

confidence: 99%

Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production

Gromada

Fiedurek

1997

J Appl Microbiol

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A . GROMADA AND J . FIEDUREK. 1997. After mutagenization and selection, mutant A.lspergillus mger strains resistant to certain agents were obtained. Seven of the mutants showed increased extracellular glucose oxidase (GOD), the level for individual cases ranged widely from 8.8 to over 138.5% in comparison with the parental strain. Studies of the relationship between method of selection and frequency of mutation showed that the highest frequency of positive mutations (15+Wo and 17.30h) was obtained from mutants resistant to ethidium bromide (1 mmol 1-I) and sodium gluconate (45"/0), respectively. The time course of growth and enzyme production by the most active mutant AM-1 1 showed intra-and extracellular GOD activities to have increased about 2.2and 24-fold, respectively, compared with the parental strain.

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Section: Resultsmentioning

confidence: 99%

Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production

Gromada

Fiedurek

1997

J Appl Microbiol

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“…The high cost of the enzyme production limits its industrial use in the production of soluble sugars. Therefore, several approaches including chemical mutations, UV irradiations and genetic engineering to obtain enhanced cellulase producing strains have been given a high priority in the last decade (Labudova and Farkas, 1983;Kotchoni and Shonukan, 2002). The enzymatic hydrolysis of cellulosic materials is a slow and complex reaction.…”

Section: Introductionmentioning

confidence: 99%

Bacillus pumilus BpCRI 6, a promising candidate for cellulase production under conditions of catabolite repression

Kotchoni¹,

Shonukan²,

Gachomo³

2003

Afr. J. Biotechnol.

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Cellulose degrading organisms have been used for the conversion of cellulolytic materials into soluble sugars or solvents in several biotechnological and industrial applications. In this report, a mutant of Bacillus pumilus was obtained after chemical mutagenesis and screened for cellulase production. This mutant named BpCRI 6 was selected for its ability to produce cellulase under catabolite repression. Cellulase yield by BpCRI 6 was four times higher than that of the wild type under optimum growth conditions (pH 6.5, 25°C and Ca 2+ 1mM). In shaking flask cultures, production of cellulase by the wild type was completely repressed in the presence of 25 mM glucose, while BpCRI 6 strain still exhibited a residual cellulase production of 80 and 40% at 25 mM and 40 mM of glucose concentrations respectively. The mutant strain is stable and grows rapidly in liquid and solid media. Under conditions of catabolite repression (40 mM of glucose), the production of cellulase by this mutant is particularly significant when compared to Trichoderma reesei a well-known cellulase producer, which is under control of end-product inhibition. This is the first report of a successful catabolite repression insensitivity of cellulase production by a mutant of B. pumilus.

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“…Resistance to the toxic glucose analogue (2-deoxyglucose) has been used as a criterion to select mutants showing increased rates of glucoamylase (Fiedurek et al 1987) and cellulase (Labudova and Farkas 1983). High levels of chymosin derivative have been isolated from the culture of , -I .…”

Section: Resultsmentioning

confidence: 99%

Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production

Gromada

Fiedurek

1997

Journal of Applied Microbiology

View full text Add to dashboard Cite

A . GROMADA AND J . FIEDUREK. 1997. After mutagenization and selection, mutant A.lspergillus mger strains resistant to certain agents were obtained. Seven of the mutants showed increased extracellular glucose oxidase (GOD), the level for individual cases ranged widely from 8.8 to over 138.5% in comparison with the parental strain. Studies of the relationship between method of selection and frequency of mutation showed that the highest frequency of positive mutations (15+Wo and 17.30h) was obtained from mutants resistant to ethidium bromide (1 mmol 1-I) and sodium gluconate (45"/0), respectively. The time course of growth and enzyme production by the most active mutant AM-1 1 showed intra-and extracellular GOD activities to have increased about 2.2-and 24-fold, respectively, compared with the parental strain.

show abstract

Enrichment technique for the selection of catabolite repression-resistant mutants of Trichoderma as producers of cellulase

Cited by 4 publications

References 11 publications

Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production

Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production

Bacillus pumilus BpCRI 6, a promising candidate for cellulase production under conditions of catabolite repression

Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production

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