Ensuring Biological Safety During Lyophilization for Microorganisms Of I–ii Pathogenicity Groups

Abstract

The article shows the presence of pathogen single cells of a dried sample in swabs taken from the camera surfaces of sublimation machines after lyophilization is completed, which may entail the possibility of release in the form of aerosol of viable cells of lyophilized microorganisms and lead to the creation of dangerous to personnel emergency situation. We proposed the use of additional isolation equipment - laminar box equipped with H14 class HEPA filters, the purpose of which is to protect the operator and the environment when working with pathogenic biological agents. The swabs taken from the internal surfaces of the laminar box and lyophilic machines at the end of the process gave negative results, which minimized the aerosol formation risk in room of the bacteriological box. We selected personal protective equipment, disinfectants for processing equipment and the final product, developed an algorithm for personnel actions, which made it possible to significantly increase the level of protection against pathogens of laboratory staff and the environment.