Enterococcus and Lactobacillus contamination of raw milk in a farm dairy environment

Kagkli, Dafni Maria; Vancanneyt, Marc; Hill, Colin; Vandamme, Peter; Cogan, Timothy M.

doi:10.1016/j.ijfoodmicro.2006.09.016

Cited by 65 publications

(48 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because udders are in frequent contact with the bedding material, we investigated whether the RAPD types present on cow teats were also present in feces and in bedding material. E. coli genotypes that are common in feces and on cow teats but are absent from milk have been identified previously (Gelsomino et al, 2001;Kagkli et al, 2007aKagkli et al, , 2007b. While Kagkli et al (2007a) recognized that cow teats are contaminated by contact with bedding material, management by appropriate husbandry practices could prevent the milk from becoming contaminated.…”

Section: W Es T/ E As T N O R T H / S O U T Hmentioning

confidence: 99%

Genetic diversity and population structure of Escherichia coli from neighboring small-scale dairy farms

et al. 2011

View full text Add to dashboard Cite

The genetic diversity and population structure of Escherichia coli isolates from small-scale dairy farms were used to assess the ability of E. coli to spread within the farm environment and between neighboring farms. A total of 164 E. coli isolates were obtained from bovine feces, bedding, cow teats and milk from 6 small-scale dairy farms. Ward's clustering grouped the isolates into 54 different random amplified polymorphic DNA (RAPD) types at 95% similarity, regardless of either the sample type or the farm of isolation. This suggests that RAPD types are shared between bovine feces, bedding, cow teats, and milk. In addition, transmission of RAPD types between the studied farms was suggested by the Ward grouping pattern of the isolates, Nei's and AMOVA population analyses, and genetic landscape shape analysis. For the first time, the latter analytical tool was used to assess the ability of E. coli to disseminate between small-scale dairy farms within the same producing region. Although a number of dispersal mechanisms could exist between farms, the genetic landscape shape analysis associated the flow of E. coli RAPD types with the movement of forage and milking staff between farms. This study will aid in planning disease prevention strategies and optimizing husbandry practices.

show abstract

Section: W Es T/ E As T N O R T H / S O U T Hmentioning

confidence: 99%

Genetic diversity and population structure of Escherichia coli from neighboring small-scale dairy farms

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Bacterial DNA isolation and digestion for pulse field gel electrophoresis (PFGE) analysis of macrorestriction fragments was performed according to Kagkli et al (2007). Restriction fragments were run in a contour-clamped homogeneous electric field electrophoresis apparatus (CHEF II, Bio-Rad) according to methods described by Argyri et al (2014).…”

mentioning

confidence: 99%

Weissella uvarum sp. nov., isolated from wine grapes

Nisiotou¹,

Dourou²,

Filippousi³

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Two bacterial strains (B18BM42 T and B18NM6) were recovered during a study of bacterial diversity on wine grapes (Vitis vinifera L.) from the Nemea region in Greece. Phylogenetic analysis based on 16S rRNA gene sequences placed the two strains within the genus Weissella, and found them to be most closely related to Weissella minor NRIC 1625 T followed by Weissella viridescens NRIC 1536 T (99.1 and 98.9 % sequence similarity, respectively). The level of DNA-DNA relatedness between strains B18NM42 T and W. minor NRIC 1625 T or W. viridescens NRIC 1536 T was 31.9 and 35.0 %, respectively. The two novel strains could be genetically differentiated from their closest relatives by REA-PFGE (restriction enzyme analysis-pulse field gel electrophoresis), RAPD (randomly amplified polymorphic DNA) and rep-PC R analyses (repetitive sequence-based PCR). Physiological examination showed that the novel strains can be distinguished from phylogenetically related species by their ability to grow at 42 6C and by certain carbohydrate fermentations. Based on the evidence above, the affiliation of the two strains to a novel species with the proposed name Weissella uvarum sp. nov. is suggested. The type strain is B18NM42 T (5DSM 28060 T 5NCCB 100484 T ).

show abstract

“…This observation is in contrast to data obtained by Martin et al (2007) who indicated an exposure of swine to multiple A. viridans strains, what is in accordance with the wide distribution of A. viridans (Woodward et al 1998), and also suggests an opportunistic pathogenic character of this bacterium. Similarly, Kagkli et al (2007) reported the presence of putative new A. viridans species in Gram-positive cocci from raw milk in a farm dairy environment. Despite being variable in antibiotic resistance profiles, A. viridans isolates from Slovakia represent homogenous group with limited genetic variability.…”

Section: Discussionmentioning

confidence: 91%

Limited genetic diversity of Aerococcus viridans strains isolated from clinical and subclinical cases of bovine mastitis in Slovakia

Špaková¹,

Elečko²,

Vasiľ³

et al. 2012

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The Aerococcus viridans isolates from bovine mastitis in Slovakia were isolated and characterized by classical microbiological and biochemical, and molecular techniques including IGS-PCR and rep-PCR, ARDRA and 16S rDNA gene sequencing. The substantial variability of antibiotic resistance patterns was observed. The majority of strains were resistant to beta-lactam antibiotics, the resistance to tetracycline was observed in 3 tested strains, resistance to lincomycin was found in 4 strains and practically all tested strains were sensitive to neomycin and ciprofloxacin. While variable at a phenotypic level, no significant genetic variability among A. viridans isolates was detected by molecular DNA based methods. The data obtained suggest that a few A. viridans strains spread among cow's population in Slovak farms.

show abstract

Enterococcus and Lactobacillus contamination of raw milk in a farm dairy environment

Cited by 65 publications

References 19 publications

Genetic diversity and population structure of Escherichia coli from neighboring small-scale dairy farms

Genetic diversity and population structure of Escherichia coli from neighboring small-scale dairy farms

Weissella uvarum sp. nov., isolated from wine grapes

Limited genetic diversity of Aerococcus viridans strains isolated from clinical and subclinical cases of bovine mastitis in Slovakia

Contact Info

Product

Resources

About