Enterococcus burkinafasonensis sp. nov. isolated from human gut microbiota

Gouba, Nina; Yimagou, Edmond Kuete; HASSANI, Yasmine; Drancourt, Michel; Fellag, Mustapha; Fonkou, Maxime Descartes Mbogning

doi:10.1016/j.nmni.2020.100702

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…M. smithii was detected by qPCR in all four of these stool samples. Finally, two new species were isolated from one TB-negative patient [ 25 , 26 , 27 ].…”

Section: Resultsmentioning

confidence: 99%

Culturomics Discloses Anti-Tubercular Enterococci Exclusive of Pulmonary Tuberculosis: A Preliminary Report

et al. 2020

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Mycobacterium tuberculosis causes pulmonary tuberculosis, a deadly infection of which the clinical expression and prognosis are not fully understood at the individual level, apart from genetic susceptibility traits. We investigated whether individual gut microbiota may correlate with pulmonary tuberculosis status. Culturomics investigations of gut microbiota in two pulmonary tuberculosis patients and two controls in Burkina Faso found 60 different bacterial species in patients and 97 in controls, including 45 in common. Further analysis of the results at the individual level indicated seven bacteria, including Enterococcus mundtii and Enterococcus casseliflavus, which were exclusively cultured in controls. Blind quantitative PCR-based exploration of faeces samples in two cohorts in Burkina Faso and in France confirmed a nonsignificant association of E. mundtii and E. casseliflavus with controls. Further in vitro explorations found four E. mundtii and E. casseliflavus strains inhibiting the growth of M. tuberculosis strains representative of four different lineages as well as Mycobacterium africanum, Mycobacterium canettii, and Mycobacterium bovis, in an inoculum-dependent manner. Heat-killed E. mundtii or E. casseliflavus were ineffective. These unprecedented observations of direct interactions between gut E. mundtii and E. casseliflavus with M. tuberculosis complex mycobacteria suggest that gut microbiota may modulate the expression of pulmonary tuberculosis.

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“…M. smithii was detected by qPCR in all four of these stool samples. Finally, two new species were isolated from one TB-negative patient [ 25 , 26 , 27 ].…”

Section: Resultsmentioning

confidence: 99%

Culturomics Discloses Anti-Tubercular Enterococci Exclusive of Pulmonary Tuberculosis: A Preliminary Report

et al. 2020

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“…The main products are lactic acid, acetic acid and formic acid, and those are also SCFAs. Enterococcus and Enterococcaceae (Byappanahalli et al, 2012;Gouba et al, 2020;Xie et al, 2021) are conditional pathogens, causing infections such as urinary tract infections, purulent abdominal infections, sepsis, endocarditis and diarrhea. On the other hand, their microbial preparations can enhance the activity of macrophage cells and promote the immune response.…”

Section: Discussionmentioning

confidence: 99%

Alterations of the Gut Microbiota in Patients With Severe Chronic Heart Failure

Sun

Du²,

et al. 2022

Front. Microbiol.

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Chronic heart failure (CHF) is the final outcome of almost all forms of cardiovascular diseases, remaining the main cause of mortality worldwide. Accumulating evidence is focused on the roles of gut microbial community in cardiovascular disease, but few studies have unveiled the alterations and further directions of gut microbiota in severe CHF patients. Aimed to investigate this deficiency, fecal samples from 29 CHF patients diagnosed with NYHA Class III-IV and 30 healthy controls were collected and then analyzed using bacterial 16S rRNA gene sequencing. As a result, there were many significant differences between the two groups. Firstly, the phylum Firmicutes was found to be remarkably decreased in severe CHF patients, and the phylum Proteobacteria was the second most abundant phyla in severe CHF patients instead of phylum Bacteroides strangely. Secondly, the α diversity indices such as chao1, PD-whole-tree and Shannon indices were significantly decreased in the severe CHF versus the control group, as well as the notable difference in β-diversity between the two groups. Thirdly, our result revealed a remarkable decrease in the abundance of the short-chain fatty acids (SCFA)-producing bacteria including genera Ruminococcaceae UCG-004, Ruminococcaceae UCG-002, Lachnospiraceae FCS020 group, Dialister and the increased abundance of the genera in Enterococcus and Enterococcaceae with an increased production of lactic acid. Finally, the alternation of the gut microbiota was presumably associated with the function including Cell cycle control, cell division, chromosome partitioning, Amino acid transport and metabolism and Carbohydrate transport and metabolism through SCFA pathway. Our findings provide the direction and theoretical knowledge for the regulation of gut flora in the treatment of severe CHF.

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“…Additionally, Romboutsia species, such as Romboutsia sedimentorum ( 31 ) and Romboutsia ilealis ( 32 ), were capable to apply carbohydrates and glucose to create short-chain fatty acids(SCFAs). In a mouse model, SCFAs inhibited hypertension-induced cardiac inflammatory responses, myocardial hypertrophy, and fibrosis ( 33 – 35 ). Previous studies have found that corticosterone can combine with and activate mineralocorticoid receptors, leading to sodium retention, which can lead to increased blood pressure ( 36 , 37 ).…”

Section: Discussionmentioning

confidence: 99%

Alteration of the gut microbiota and metabolite phenylacetylglutamine in patients with severe chronic heart failure

Zhang

Cai

Sun

et al. 2023

Front. Cardiovasc. Med.

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Chronic Heart Failure (CHF) is the end result of nearly all cardiovascular disease and is the leading cause of deaths worldwide. Studies have demonstrated that intestinal flora has a close relationship with the development of Cardiovascular Disease (CVD) and plays a vital role in the disease evolution process. Phenylacetylglutamine (PAGln) a metabolite of the intestinal flora, is one of the common chronic kidney disease toxins. Its concentrations in plasma were higher in patients with major adverse cardiovascular events (MACE) however, its variation in patients with various degrees of CHF has rarely been reported. Therefore, we collected stool and plasma samples from 22 healthy controls, 29 patients with NYHA Class III and 29 patients with NYHA Class IV CHF (NYHA stands for New York Heart Association) from the Department of Cardiology of Shanghai Fengxian District Central Hospital. Next, we analyzed these samples by performing bacterial 16S ribosomal RNA gene sequencing and liquid chromatography tandem mass spectrometry. The result shows: The Chao 1 index was significantly lower in both NYHA class III and NYHA class IV than it was in the control group. The beta diversity was substantially dissimilar across the three groups. The linear discriminant analysis effect size analysis (LEfSe) showed that the bacterial species with the largest differences were Lachnospiraceae in control group, Enterobacteriaceae in NYHA class III, and Escherichia in NYHA class IV. The concentration of PAGln was significantly different between CHF and control groups and increased with the severity of heart failure. Finally, the correlation analysis represented that Parabacteroides and Bacteroides were negatively correlated to brain natriuretic peptide (BNP) and PAGln; Romboutsia and Blautia adversely associated with PAGln; Klebsiella was positively interrelated with BNP; Escherichia-Shigella was positively correlated with PAGln and BNP; Alistipes was contrasted with BNP; and Parabacteroides was negatively correlated with the left ventricular end-diastolic diameter (LVEDD). This study presented that the intestinal flora and its metabolite PAGln were altered with different grades of CHF and illustrated the effects of the gut flora and its metabolite on CHF.

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Enterococcus burkinafasonensis sp. nov. isolated from human gut microbiota

Cited by 7 publications

References 17 publications

Culturomics Discloses Anti-Tubercular Enterococci Exclusive of Pulmonary Tuberculosis: A Preliminary Report

Culturomics Discloses Anti-Tubercular Enterococci Exclusive of Pulmonary Tuberculosis: A Preliminary Report

Alterations of the Gut Microbiota in Patients With Severe Chronic Heart Failure

Alteration of the gut microbiota and metabolite phenylacetylglutamine in patients with severe chronic heart failure

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