Enteropathogens Associated with Acute and Persistent Diarrhea in BangladeshiChildren <5 Years of Age

Abstract

A longitudinal study of diarrhea was carried out from May 1988 to April 1989 by household surveillance of 705 children less than 5 years old in rural Bangladesh. Stool samples were examined for enteric pathogens at the beginning of each diarrheal episode. For persistent episodes, stool examination was repeated on days 15-17 of the illness. For each case of persistent diarrhea, stool samples from age-matched acute diarrheal and healthy controls were examined. Compared with healthy controls, cases of diarrhea were associated with Shigella species (P = .07) and rotavirus (P less than .05). Diffusely adherent Escherichia coli (P less than .05) and cryptosporidia (P = .07) were the only enteropathogens associated with persistent diarrhea in comparison with acute diarrhea. No more than 15% of children had the same class of pathogen identified from stool on both days 1-3 and days 15-17, indicating that persistent infection was uncommon. However, a different enteropathogen was frequently found on days 15-17, suggesting that sequential infection may be a cause of persistent diarrhea.

Interleukin‐8 Secretion by Epithelial Cells Infected with Diffusely Adherent Escherichia coli Possessing Afa Adhesin‐Coding Genes

“…Their role in diarrheal disease, however, has been controversial (11,18,25). Several groups have used age-dependent susceptibility to explain epidemiological discrepancies, since a positive association with diarrhea was found when study populations were age-stratified (1,18,20,35,47).Clinical microbiologists have to hesitate to classify the DAEC isolates as causative agents. DAEC probably comprise a heterogeneous group of organisms with variable enteropathogenicity (45).…”

High Resolution Studies of the Afa/Dr Adhesin DraE and Its Interaction with Chloramphenicol

“…Diffusely adherent Escherichia coli has recently been accepted as a diarrheagenic strain of E. coli that causes watery diarrhea in young children (1)(2)(3). Diffusely adherent E. coli shares a characteristic pattern of infection of epithelial cells in which the bacteria form a diffuse pattern around the host cells and is accompanied by an elongation of the microvilli (4).…”

Usage of Silver-Stained Polyacrylamide Gels Electrophoresis (PAGE) for Detection of Rotavirus Infection by Direct Identification of Viral Nucleic Acid

“…Some of the authors 14,21 showed an association of bacterial pathogens with rotavirus positive cases. Various enteropathogens isolated in their study 25,26 . The PAGE system used in this study was very simple to perform and the results were available on the same day.…”