Enterotoxigenic Escherichia coli associated with childhood diarrhoea in Colombia, South America

Gómez‐Duarte, Oscar G.; Romero-Herazo, Yesenia; Páez-Canro, Carol; Eslava-Schmalbach, Javier; Arzuza, Octavio

doi:10.3855/jidc.2667

Cited by 22 publications

(19 citation statements)

References 38 publications

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“…Seven of these ETEC strains came from children with diarrhea from a prevalence study previously described in two Caribbean cities in Colombia [29]. The remaining ETEC strains were obtained from case-control studies of children less than 5 years of age also in Cartagena, Colombia [28]. Twenty-five strains were from cases and 8 strains from healthy controls (Table S1).…”

Section: Methodsmentioning

confidence: 99%

“…ETEC is the leading cause of diarrhea in children less than 5 years of age in Colombia, South America [28, 29], yet no information is available of the phenotypes and genotypes associated with these strains. The objectives of this study were to identify the most common genotypes associated with Colombian ETEC clinical isolates with respect to enterotoxins, CSs, nonclassical virulence genes, and genomic profiles and to determine the most common ETEC O:H serotypes and antimicrobial susceptibility patterns.…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic and Genotypic Characterization of EnterotoxigenicEscherichia coliClinical Isolates from Northern Colombia, South America

Guerra

Romero-Herazo

Arzuza

et al. 2014

BioMed Research International

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Enterotoxigenic Escherichia coli (ETEC) are major causes of childhood diarrhea in low and middle income countries including Colombia, South America. To understand the diversity of ETEC strains in the region, clinical isolates obtained from northern Colombia children were evaluated for multiple locus sequencing typing, serotyping, classical and nonclassical virulence genes, and antibiotic susceptibility. Among 40 ETEC clinical isolates evaluated, 21 (52.5%) were positive for LT gene, 13 (32.5%) for ST gene, and 6 (15%) for both ST and LT. The most prevalent colonization surface antigens (CS) were CS21 and CFA/I identified in 21 (50%) and 13 (32.5%) isolates, respectively. The eatA, irp2, and fyuA were the most common nonclassical virulence genes present in more than 60% of the isolates. Ampicillin resistance (80% of the strains) was the most frequent phenotype among ETEC strains followed by trimethoprim-sulfamethoxazole resistance (52.5%). Based on multiple locus sequencing typing (MLST), we recognize that 6 clonal groups of ETEC clinical isolates circulate in Colombia. ETEC clinical isolates from children in northern Colombia are highly diverse, yet some isolates circulating in the community belong to well-defined clonal groups that share a unique set of virulence factors, serotypes, and MLST sequence types.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of EnterotoxigenicEscherichia coliClinical Isolates from Northern Colombia, South America

Guerra

Romero-Herazo

Arzuza

et al. 2014

BioMed Research International

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“…The inclusion criteria for the study participants were: (a) Being a child less than five years of age; (b) Having diarrhoea that fulfil the WHO criteria for ADD [13,14]. Those having a history of antibiotics use two weeks before enrolment were excluded from the study.…”

Section: Methodsmentioning

confidence: 99%

Antibiotic Susceptibility Profile of Escherichia coli and Salmonella Causing Childhood Diarrhoea in Awka Municipality, South-eastern Nigeria

Mc¹,

Gi²,

Cp³

et al. 2017

Clin Microbiol

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“…A recent case-control study reported a high prevalence of E. coli diseases in Cartagena among children less than 5 year old (Gomez-Duarte et al, 2013). It led us to ask whether foodborne and/or waterborne pathogens are playing a role in the epidemiology of E. coli intestinal illnesses.…”

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confidence: 99%

Genetic Relatedness Among Escherichia coli Pathotypes Isolated from Food Products for Human Consumption in Cartagena, Colombia

Amézquita-Montes

Tamborski

Kopsombut

et al. 2015

Foodborne Pathogens and Disease

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Foodborne pathogens are a leading cause of mild-to-severe gastrointestinal illnesses worldwide. Escherichia coli pathotypes have been known to cause gastrointestinal illnesses in children less than 5 years old in Colombia. However, insufficient information is available on the prevalence of E. coli contamination of food products and the kind of E. coli food product reservoirs. The two objectives of this study were designed to address this issue. The first objective was to ascertain coliform, E. coli, and pathogenic E. coli contamination of food products readily available for human consumption in Cartagena, Colombia. The second objective was to evaluate the relationship between pathogenic E. coli isolated from food products and those isolated from cases of diarrhea in children. Food product samples consisting of pasteurized milk, unpasteurized fruit juice, ground beef, cheese, and vegetables were obtained at four retail stores. The food samples were cultured in liquid media and tested for the presence of coliforms and E. coli. E. coli isolates were tested by polymerase chain reaction for the presence of pathogenic E. coli. Coliforms, E. coli, and E. coli intestinal pathotypes contamination were detected in 88.4%, 53%, and 2.1% of food product samples, respectively. Ground beef and cheese were the only food samples contaminated with E. coli intestinal pathotypes including enteropathogenic (EPEC), Shiga toxinproducing (STEC), and enterotoxigenic E. coli (ETEC). Closed multilocus sequencing typing relationships between diarrheagenic E. coli isolates from food products and from individuals with diarrhea suggest that food products readily available at public markets in Cartagena can transmit ETEC and possibly EPEC and STEC. We demonstrated that a high proportion of food products for human consumption available at public markets in Cartagena are contaminated with coliforms, E. coli, and E. coli intestinal pathogens. Furthermore, food products containing E. coli intestinal pathogens may be involved in the transmission of foodborne illnesses among children in Cartagena, Colombia.

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Enterotoxigenic Escherichia coli associated with childhood diarrhoea in Colombia, South America

Cited by 22 publications

References 38 publications

Phenotypic and Genotypic Characterization of EnterotoxigenicEscherichia coliClinical Isolates from Northern Colombia, South America

Phenotypic and Genotypic Characterization of EnterotoxigenicEscherichia coliClinical Isolates from Northern Colombia, South America

Antibiotic Susceptibility Profile of Escherichia coli and Salmonella Causing Childhood Diarrhoea in Awka Municipality, South-eastern Nigeria

Genetic Relatedness Among Escherichia coli Pathotypes Isolated from Food Products for Human Consumption in Cartagena, Colombia

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