Enterovirus in arteriosclerosis: A pilot study

Cytomegalovirus Load in Inflamed Intestinal Tissue Is Predictive of Resistance to Immunosuppressive Therapy in Ulcerative Colitis

“…Th e results were expressed in copies of CMV genome per mg of tissue. Extraction and amplifi cation steps were controlled for the absence of Taq polymerase inhibitors by the amplifi cation of the human aldolase gene as previously described ( 27 ). Th e molecular data were all interpreted by a single virologist (S.P.).…”

Prevalence of Cytomegalovirus Infection in Steroid-refractory Crohnʼs Disease

“…Extraction and amplification steps were controlled for the absence of Taq polymerase inhibitors by amplification of the human aldolase gene as previously described. 9 A total of 60 consecutive patients were enrolled in the study, including 42 UC cases and 18 CD cases. The characteristics of the UC patients have been reported.…”

Multicenter evaluation of the ENTEROVIRUS R-gene™ real-time RT-PCR assay for the detection of enteroviruses in clinical specimens

“…In the Saint-Etienne laboratory, HEV amplification was performed using 10 l of RNA as previously described. 17 In the Lyon laboratory, 5 l of RNA were added to a 15 l mixture containing 0.5 M of EV primer (5 -CACYGGATGGCC-3 ), 0.5 mM dNTPs and 5 U of AMV-RT (Promega). Reverse transcription was performed using a GenAmp …”