Neuroscience and biological evidence emphasizes the profound influence of natural light on human health, offering benefits such as reducing fatigue, heightened alertness in healthcare providers, and improving patient outcomes. The objective of this review is to identify scientific studies and research to evaluate and report evidence of indoor lighting conditions’ influence on health outcomes, which can be used to develop lighting designs that align circadian rhythms in healthcare settings. A comprehensive search was conducted to identify rigorous empirical studies focused on the link between interior lighting conditions with health outcomes in the healthcare environment. For a comprehensive review of the existing literature, a four-phased methodology was employed including literature search, screening, and selection. Literature appraisals were conducted to determine the relevance and quality of evidence for each study identified. In addition, using a thematic analysis, patterns were identified, analyzed, and interpreted within the literature review. Accordingly, the results were organized into two main groups interventional human subjects and simulation-based studies. Despite evidence that natural light influences human health and happiness, a synthesis of reviewed studies suggests that the evidence for the benefits of artificial lighting in healthcare settings is less conclusive, with potential factors including variations in lighting design, inconsistent implementation of lighting interventions, and differing sample populations across studies. We conclude with an executive summary suggesting that future research should use standardized metrics and methods to focus on bridging the gap between theoretical understanding and practical application in lighting design for healthcare environments. Collaboration among architects, designers, lighting experts, and healthcare professionals can address these factors contributing to building a stronger evidence-based design for the benefits of artificial lighting in healthcare settings.