Entropy-driven amplified electrochemiluminescence biosensor for RdRp gene of SARS-CoV-2 detection with self-assembled DNA tetrahedron scaffolds

Fan, Zhenqiang; Yao, Bo; Ding, Yuedi; Zhao, Jing; Xie, Minhao; Zhang, Kai

doi:10.1016/j.bios.2021.113015

Cited by 121 publications

(72 citation statements)

References 44 publications

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“…Electrochemical-based methods conjugated nanotechnology is promising for several reasons [266]. They can be used for gene detection of (1) gene such as nucleocapsid phosphoprotein (N-gene) [226], RdRp gene [267]; (2) antigen [234]; and (3) antibodies. The method can be easily implemented into portable devices offering simple operation and on-site detection [227].…”

Section: Discussionmentioning

confidence: 99%

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

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A sensitive method for diagnosing coronavirus disease 2019 (COVID-19) is highly required to fight the current and future global health threats due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV 2). However, most of the current methods exhibited high false-negative rates, resulting in patient misdiagnosis and impeding early treatment. Nanoparticles show promising performance and great potential to serve as a platform for diagnosing viral infection in a short time and with high sensitivity. This review highlighted the potential of nanoparticles as platforms for the diagnosis of COVID-19. Nanoparticles such as gold nanoparticles, magnetic nanoparticles, and graphene (G) were applied to detect SARS-CoV 2. They have been used for molecular-based diagnosis methods and serological methods. Nanoparticles improved specificity and shorten the time required for the diagnosis. They may be implemented into small devices that facilitate the self-diagnosis at home or in places such as airports and shops. Nanoparticles-based methods can be used for the analysis of virus-contaminated samples from a patient, surface, and air. The advantages and challenges were discussed to introduce useful information for designing a sensitive, fast, and low-cost diagnostic method. This review aims to present a helpful survey for the lesson learned from handling this outbreak to prepare ourself for future pandemic.

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Section: Discussionmentioning

confidence: 99%

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

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“…The immuno-modulatroy response generated by CpG motif of DNA nanostructures via generating greater level of secretion of various pro-inflammatory cytokines including interleukins (IL-6, 12), tumor necrosis factor (TNF-α) is more significant and stronger than those generated via single-stranded CpG oligonucleotides [ 191 ]. The DNA tetrahedral carriers were small in size and are stable under normal physiological conditions and showed no toxicity, representing the promising applications in immunotherapy [ 192 ]. Liedl and co-workers developed 30-helix DNA origami nanostructures as the cargo for CpG motif delivery.…”

Section: Potential Immuno-modulatory Effect Of Dna-based Nanostructuresmentioning

confidence: 99%

DNA Based and Stimuli-Responsive Smart Nanocarrier for Diagnosis and Treatment of Cancer: Applications and Challenges

Sabir

Zeeshan

Laraib

et al. 2021

Cancers

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The rapid development of multidrug co-delivery and nano-medicines has made spontaneous progress in tumor treatment and diagnosis. DNA is a unique biological molecule that can be tailored and molded into various nanostructures. The addition of ligands or stimuli-responsive elements enables DNA nanostructures to mediate highly targeted drug delivery to the cancer cells. Smart DNA nanostructures, owing to their various shapes, sizes, geometry, sequences, and characteristics, have various modes of cellular internalization and final disposition. On the other hand, functionalized DNA nanocarriers have specific receptor-mediated uptake, and most of these ligand anchored nanostructures able to escape lysosomal degradation. DNA-based and stimuli responsive nano-carrier systems are the latest advancement in cancer targeting. The data exploration from various studies demonstrated that the DNA nanostructure and stimuli responsive drug delivery systems are perfect tools to overcome the problems existing in the cancer treatment including toxicity and compromised drug efficacy. In this light, the review summarized the insights about various types of DNA nanostructures and stimuli responsive nanocarrier systems applications for diagnosis and treatment of cancer.

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“…DNA tetrahedron (DT) on the surface of the electrode is modified for the design of the biosensor. Biosensor shows an "ECL on" state with LOD up to 2.67 fM ( 89 ).…”

Section: Recently Developed Exclusive Interfaces-based Biosensors For Sars-cov-2 Testing and Covid-19 Diagnosismentioning

confidence: 99%

State-of-the-art colloidal particles and unique interfaces-based SARS-CoV-2 detection methods and COVID-19 diagnosis

Saatçi

Natarajan

2021

Current Opinion in Colloid & Interface Science

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In March 2020, SARS-CoV-2 based infections were declared ‘COVID-19 pandemic’ by the World Health Organization (WHO). Pandemic raised the necessity to design and develop genuine and sensitive tests for precise specific SARS-CoV-2 infections detection. Nanotechnological methods offer new ways to fight COVID-19. Nanomaterials are ideal for unique sensor platforms due to their easy manufacturing, chemically versatile properties. In this context, selected examples for integrating nanomaterials and distinct biosensor platforms are given to detect SARS-CoV-2 biological materials and COVID-19 biomarkers, giving researchers and scientists more goals and a better forecast to design more relevant and novel sensor arrays for COVID-19 diagnosis.

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Entropy-driven amplified electrochemiluminescence biosensor for RdRp gene of SARS-CoV-2 detection with self-assembled DNA tetrahedron scaffolds

Cited by 121 publications

References 44 publications

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

DNA Based and Stimuli-Responsive Smart Nanocarrier for Diagnosis and Treatment of Cancer: Applications and Challenges

State-of-the-art colloidal particles and unique interfaces-based SARS-CoV-2 detection methods and COVID-19 diagnosis

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