Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT)

Xie, Shijun; Zhang, Xiaojun; Zhang, Jiquan; Li, Fuhua; Xiang, Jianhai

doi:10.1371/journal.pone.0144922

Cited by 41 publications

(20 citation statements)

References 26 publications

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“…However, all of these genes were down regulated by ammonia stress in the shrimp ( S3 Table ). Xie et al [ 91 ] reported that the envelope proteins of WSSV interact with peritrophin protein in the stomach when WSSV infecting the L . vannamei , so the up-regulated expression of peritrophin by ammonia stress would increase the susceptibility to pathogens.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (Litopenaeus vannamei) under Acute Ammonia Stress

Lu¹,

Kong²,

Luan³

et al. 2016

PLoS ONE

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In the practical farming of Litopenaeus vannamei, the intensive culture system and environmental pollution usually results in a high concentration of ammonia, which usually brings large detrimental effects to shrimp, such as increasing the susceptibility to pathogens, reducing growth, decreasing osmoregulatory capacity, increasing the molting frequency, and even causing high mortality. However, little information is available on the molecular mechanisms of the detrimental effects of ammonia stress in shrimp. In this study, we performed comparative transcriptome analysis between ammonia-challenged and control groups from the same family of L. vannamei to identify the key genes and pathways response to ammonia stress. The comparative transcriptome analysis identified 136 significantly differentially expressed genes that have high homologies with the known proteins in aquatic species, among which 94 genes are reported potentially related to immune function, and the rest of the genes are involved in apoptosis, growth, molting, and osmoregulation. Fourteen GO terms and 6 KEGG pathways were identified to be significantly changed by ammonia stress. In these GO terms, 13 genes have been studied in aquatic species, and 11 of them were reported potentially involved in immune defense and two genes were related to molting. In the significantly changed KEGG pathways, all the 7 significantly changed genes have been reported in shrimp, and four of them were potentially involved in immune defense and the other three were related to molting, defending toxicity, and osmoregulation, respectively. In addition, majority of the significantly changed genes involved in nitrogen metabolisms that play an important role in reducing ammonia toxicity failed to perform the protection function. The present results have supplied molecular level support for the previous founding of the detrimental effects of ammonia stress in shrimp, which is a prerequisite for better understanding the molecular mechanism of the immunosuppression from ammonia stress.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (Litopenaeus vannamei) under Acute Ammonia Stress

Lu¹,

Kong²,

Luan³

et al. 2016

PLoS ONE

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“…Curiously, we also observed a chitin metabolism-related gene, peritrophin-1 or A, among the immune-related genes expressed in the hepatopancreas of healthy shrimps. Peritrophin may interact with many proteins from the White Spot virus envelope and mediate its infection in Exopalaemon carinicauda [82] and L. vannamei [83,84] after virus injection. Studies have already reported that WSSV may have access to shrimp body through oral or waterborne routes [47,48].…”

Section: Discussionmentioning

confidence: 99%

Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure

Santos

Andrade

Fernandes

et al. 2020

Genes

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White Spot Syndrome Virus (WSSV) is one of the main threats to farming Litopenaeus vannamei, the most important crustacean commercialized in aquaculture worldwide. Here, we performed RNA-seq analyses in hepatopancreas and muscle from WSSV-negative (healthy) and WSSV-positive (unhealthy) L. vannamei, previously exposed to the virus, to obtain new insights about the molecular basis of resistance to WSSV. We detected 71% of our reads mapped against the recently described L. vannamei genome. This is the first report mapping RNA-seq transcripts from shrimps exposed to WSSV against the species reference genome. Differentially expressed gene (DEG) analyses were performed for four independent comparisons, and 13,338 DEGs were identified. When the redundancies and isoforms were disregarded, we observed 8351 and 6514 DEGs, respectively. Interestingly, after crossing the data, we detected a common set of DEGs for hepatopancreas and healthy shrimps, as well as another one for muscle and unhealthy shrimps. Our findings indicate that genes related to apoptosis, melanization, and the Imd pathway are likely to be involved in response to WSSV, offering knowledge about WSSV defense in shrimps exposed to the virus but not infected. These data present potential to be applied in further genetic studies in penaeids and other farmed shrimp species.

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“…According to a study by Xie et al (2015), the P. vannamei peritrophin-like protein ( Lv PT) was found to interact with VP37, an envelope protein of the white spot syndrome virus. Further studies using the yeast two-hybrid (Y2H) library that was constructed using cDNA obtained from the stomach and gut of P. vannamei revealed that Lv PT could also interact with other WSSV envelope proteins such as VP32, VP38A, VP39B, and VP41A.…”

Section: Discussionmentioning

confidence: 99%

“…VP37, found on the outside of the virion WSSV membrane protein complex (Chang et al, 2010), played a major role in WSSV infection (Wu, Wang & Zhang, 2005). This envelope protein interacts with receptors or assisting proteins on the peritrophic membrane (PM), possibly peritrophin, that can enable the WSSV to break through the physical barrier of the PM (Xie et al, 2015). PM only allows particles smaller than 20 nm to pass through the membrane (Martin et al, 2006), wherein, the size and length of WSSV is 70–150 nm and 250–380 nm, respectively (Lu et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

A new insight to biomarkers related to resistance in survived-white spot syndrome virus challenged giant tiger shrimp,Penaeus monodon

Ghani

Bhassu

2019

PeerJ

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The emergence of diseases such as white spot disease has become a threat to Penaeus monodon cultivation. Although there have been a few studies utilizing RNA-Seq, the cellular processes of host-virus interaction in this species remain mostly anonymous. In the present study, P. monodon was challenged with WSSV by intramuscular injection and survived for 12 days. The effect of the host gene expression by WSSV infection in the haemocytes, hepatopancreas and muscle of P. monodon was studied using Illumina HiSeq 2000. The RNA-Seq of cDNA libraries was developed from surviving WSSV-challenged shrimp as well as from normal healthy shrimp as control. A comparison of the transcriptome data of the two groups showed 2,644 host genes to be significantly up-regulated and 2,194 genes significantly down-regulated as a result of the infection with WSSV. Among the differentially expressed genes, our study discovered HMGB, TNFSF and c-Jun in P. monodon as new potential candidate genes for further investigation for the development of potential disease resistance markers. Our study also provided significant data on the differential expression of genes in the survived WSSV infected P. monodon that will help to improve understanding of host-virus interactions in this species.

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Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT)

Cited by 41 publications

References 26 publications

Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (Litopenaeus vannamei) under Acute Ammonia Stress

Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (Litopenaeus vannamei) under Acute Ammonia Stress

Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure

A new insight to biomarkers related to resistance in survived-white spot syndrome virus challenged giant tiger shrimp,Penaeus monodon

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