Environmental analysis of single cells

Zhao, Qiang; Le, X. Chris

doi:10.1007/s00216-006-0672-2

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…To prevent poor formation of the chromatographic peaks of phenols, a derivatization step is essential to improve chromatographic performance and achieve better results than conventional methods without a derivatization step. In situ acetylation of phenols has been applied as a derivatization process in previous studies [36][37][38]. Thus, in situ acetylation of phenols with acetic anhydride in the presence of potassium carbonate was selected for the present experiments because of the high performance of the technique in aqueous media, low time consumption, high extraction efficiency, and low cost [37,39].…”

Section: Derivatizationmentioning

confidence: 99%

Accelerated solvent extraction combined with dispersive liquid–liquid microextraction before gas chromatography with mass spectrometry for the sensitive determination of phenols in soil samples

Xing

Wang

Chen

et al. 2015

J of Separation Science

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A method combining accelerated solvent extraction with dispersive liquid-liquid microextraction was developed for the first time as a sample pretreatment for the rapid analysis of phenols (including phenol, m-cresol, 2,4-dichlorophenol, and 2,4,6-trichlorophenol) in soil samples. In the accelerated solvent extraction procedure, water was used as an extraction solvent, and phenols were extracted from soil samples into water. The dispersive liquid-liquid microextraction technique was then performed on the obtained aqueous solution. Important accelerated solvent extraction and dispersive liquid-liquid microextraction parameters were investigated and optimized. Under optimized conditions, the new method provided wide linearity (6.1-3080 ng/g), low limits of detection (0.06-1.83 ng/g), and excellent reproducibility (<10%) for phenols. Four real soil samples were analyzed by the proposed method to assess its applicability. Experimental results showed that the soil samples were free of our target compounds, and average recoveries were in the range of 87.9-110%. These findings indicate that accelerated solvent extraction with dispersive liquid-liquid microextraction as a sample pretreatment procedure coupled with gas chromatography and mass spectrometry is an excellent method for the rapid analysis of trace levels of phenols in environmental soil samples.

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Section: Derivatizationmentioning

confidence: 99%

Accelerated solvent extraction combined with dispersive liquid–liquid microextraction before gas chromatography with mass spectrometry for the sensitive determination of phenols in soil samples

Xing

Wang

Chen

et al. 2015

J of Separation Science

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“…Comet assay has been expanded to many application fields such as human biomonitoring, gene–environment interactions, drug screening, and genotoxicity testing. It has advantages of low cost and ease of access. − In addition, the results of comet assay reflect the integrated cellular response to different extracellular environments.…”

mentioning

confidence: 99%

Ultrahigh-Throughput Approach for Analyzing Single-Cell Genomic Damage with an Agarose-Based Microfluidic Comet Array

Feng

et al. 2013

Anal. Chem.

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Genomic DNA damage was generally identified with a "comet assay" but limited by low throughput and poor reproducibility. Here we demonstrated an ultrahigh-throughput approach with a microfluidic chip to simultaneously interrogate DNA damage conditions of up to 10,000 individual cells (approximately 100-fold in throughput over the conventional method) with better reproducibility. For experiment, agarose was chosen as the chip fabrication material, which would further act as an electrophoretic sieving matrix for DNA fragments separation. Cancer cells (HeLa or HepG2) were lined up in parallel microchannels by capillary effect to form a dense array of single cells. After treatment with different doses of hydrogen peroxide, individual cells were then lysed for subsequent single-cell gel electrophoresis in the direction vertical to microchannel and fluorescence detection. Through morphological analysis and fluorescent measurement of comet-shaped DNA, the damage conditions of individual cells could be quantified. DNA repair capacity was further evaluated to validate the reliability of this method. It indicated that the agarose-based microfluidic comet array electrophoresis was simple, highly reproducible, and of high throughput, providing a new method for highly efficient single-cell genomic analysis.

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Cellular distribution and degradation of cobalt ferrite nanoparticles in Balb/3T3 mouse fibroblasts

et al. 2011

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Environmental analysis of single cells

Cited by 4 publications

References 30 publications

Accelerated solvent extraction combined with dispersive liquid–liquid microextraction before gas chromatography with mass spectrometry for the sensitive determination of phenols in soil samples

Accelerated solvent extraction combined with dispersive liquid–liquid microextraction before gas chromatography with mass spectrometry for the sensitive determination of phenols in soil samples

Ultrahigh-Throughput Approach for Analyzing Single-Cell Genomic Damage with an Agarose-Based Microfluidic Comet Array

Cellular distribution and degradation of cobalt ferrite nanoparticles in Balb/3T3 mouse fibroblasts

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