Environmental and cortisol-mediated control of Ca2+ uptake in tilapia (Oreochromis mossambicus)

Lin, Cheng Huang; Kuan, Wei-Chun; Liao, Bokai; Deng, Ang-Ni; Tseng, Deng-Yu; Hwang, Pung‐Pung

doi:10.1007/s00360-016-0963-7

Cited by 23 publications

(26 citation statements)

References 47 publications

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“…Similarly, we observed contrasting DE of two epithelial Ca 2+ channel genes (ECaC), TRPV2 and TRPV6 , which convey Ca 2+ down electrochemical gradients. However, we did not observe changes in expression of either the basolateral Na + /Ca 2+ exchanger, NCX ( SLC8A1 ), or Ca 2+ -ATPase, PMCA ( ATP2B ), that were hypothesized to facilitate Ca 2+ uptake in zebrafish and tilapia in coordination with ECaC [3,67]. Consistent with their role in Na + , K + , and Cl − secretion in Omoss in seawater [68], we observed expression of one of two paralogs of the Na + -K + -Cl − co-transporter, NKCC ( SLC12A2 ), down-regulated in blackwater, whereas the apical chloride channel CFTR was only present in the raw transcriptome.…”

Section: Discussionmentioning

confidence: 99%

Osmoregulation in freshwaters: Gene expression in the gills of a Neotropical cichlid in contrasting pH and ionic environments

Willis

Saenz

Wang

et al. 2018

Preprint

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22 24 25 Abstract 26 27 Freshwater habitats of the Neotropics exhibit a gradient from relatively neutral, ion-rich 28 whitewater to acidic, ion-poor blackwater. Closely related species often show 29 complementary distributions among ionic habitats, suggesting that adaptation to divergent 30 osmoregulatory environments may be an important driver of Neotropical fish diversity. 31 However, little is known about the evolutionary tradeoffs involved in ionoregulation across 32 distinct freshwater environments. Here, we surveyed gill mRNA expression of Cichla 33 ocellaris var. monoculus, a Neotropical cichlid, to examine cellular and physiological 34 responses to experimental conditions mimicking whitewater and blackwater.35 Gene ontology enrichment of expressed genes indicated that the gills were remodeled 36 during both forms of environmental challenge, with changes biased towards the cellular 37 membrane. We observed expression of signaling pathways from both the acute and 38 extended response phases, including evidence that growth hormone (GH) may mediate 39 osmoregulation in whitewater through paracrine expression of insulin-like growth factor I 40 (IGF-I), but not through the GH receptor, which instead showed correlated up-regulation 41 with the prolactin receptor and insulin-like growth factor II in blackwater.42 Differential expression of genes related to paracellular tight junctions and transcellular ion 43 transport showed responses similar to euryhaline fishes in fresh versus seawater, with 44 some exceptions, suggesting that relaxed ion retention via the gills, possibly mediated by 45 the GH/IGF-I axis, is a strong candidate for evolutionary modification in whitewater and 46 blackwater endemic populations. In each osmoregulatory domain, we saw examples of 3 47 contrasting differential expression of paralogs of genes that are single copy in most 48 terrestrial vertebrates, indicating that adaption by fishes to diverse physicochemcial 49 environments has capitalized on diversification of osmoregulatory gene families. 50 51 53 54 Running title: gene expression across an Amazon ionic gradient 4 55 56 Introduction 57 58Gill surfaces of fishes must be thin in order to facilitate gas exchange, but this also 59 promotes rapid gain or loss of ions and water with the environment -a functional tradeoff 60 called the 'osmo-respiratory compromise' [1]. Management of osmotic and ionic stress is 61 therefore a major biological challenge for fishes and has been estimated to account for 2-62 20% of resting metabolic rate [2]. A great deal has been learned about mechanisms 63 involved in osmoregulation from studies of euryhaline fishes exposed to salinity gradients 64 [3]; however, these fishes represent a small fraction of fish diversity, as the vast majority 65 are stenohaline. Among these, freshwater fishes are challenged to maintain homeostasis in 66 the face of wide variation in solute concentration, pH, and other chemical factors, but are 67 less well-studied than their euryhaline counterparts [4]. Moreover, it is clear...

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Section: Discussionmentioning

confidence: 99%

Osmoregulation in freshwaters: Gene expression in the gills of a Neotropical cichlid in contrasting pH and ionic environments

Willis

Saenz

Wang

et al. 2018

Preprint

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“…In situ hybridization was performed following a previous study ( Lin et al, 2016 ). Briefly, gills were fixed overnight with 4% paraformaldehyde in PBS.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Calcium Balancing Strategies During Hypothermic Acclimation of Tilapia (Oreochromis mossambicus) and Goldfish (Carassius auratus)

Han

Tsai

et al. 2018

Front. Physiol.

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The body temperatures of teleost species fluctuate following changes in the aquatic environment. As such, decreased water temperature lowers the rates of biochemical reactions and affects many physiological processes, including active transport-dependent ion absorption. Previous studies have focused on the impacts of low temperature on the plasma ion concentrations or membrane transporters in fishes. However, very few in vivo or organism-level studies have been performed to more thoroughly elucidate the process of acclimation to low temperatures. In the present study, we compared the strategies for cold acclimation between stenothermic tilapia and eurythermic goldfish. Whole-body calcium content was more prominently diminished in tilapia than in goldfish after long-term cold exposure. This difference can be attributed to alterations in the transportation parameters for Ca2+ influx, i.e., maximum velocity (Vmax) and binding affinity (1/Km). There was also a significant difference in the regulation of Ca2+ efflux between the two fishes. Transcript levels for Ca2+ related transporters, including the Na+/Ca2+ exchanger and epithelial Ca2+ channel, were similarly regulated in both fishes. However, upregulation of plasma membrane Ca2+ATPase expression was more pronounced in goldfish than in tilapia. In addition, enhanced Na+/K+-ATPase abundance, which provides the major driving force for ion absorption, was only detected in tilapia, while upregulated Na+/K+-ATPase activity was only detected in goldfish. Based on the results of the present study, we have found that goldfish and tilapia differentially regulate gill epithelial plasma membrane Ca2+-ATPase (PMCA) expression and Na+/K+-ATPase activity in response to cold environments. These regulatory differences are potentially linked to more effective regulation of Ca2+ influx kinetics and better maintenance of whole body calcium content in goldfish than in tilapia.

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“…Moreover, cortisol treatment was shown to induce in vitro calcium transport in cultured rainbow trout gill epithelium, which supports its hypercalcemic role ( 48 ). Also, tilapia exposed to exogenous cortisol showed an increase in calcium uptake and upregulation of epithelial Ca 2+ channel ( ecac ) gene expression ( 49 ). It would therefore appear that teleost fish regulate the calcium uptake to cope with a fluctuating water environment which is closely related to bone homeostasis.…”

Section: Stress-induced Glucocorticoid Effects On Bone Mineral Metabomentioning

confidence: 99%

Stress, Glucocorticoids and Bone: A Review From Mammals and Fish

2018

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Glucocorticoids (GCs) are the final effector products of a neuroendocrine HPA/HPI axis governing energy balance and stress response in vertebrates. From a physiological point of view, basal GC levels are essential for intermediary metabolism and participate in the development and homeostasis of a wide range of body tissues, including the skeleton. Numerous mammalian studies have demonstrated that GC hormones exert a positive role during bone modeling and remodeling as they promote osteoblastogenesis to maintain the bone architecture. Although the pharmacological effect of the so-called stress hormones has been widely reported, the role of endogenous GCs on bone mineral metabolism as result of the endocrine stress response has been largely overlooked across vertebrates. In addition, stress responses are variable depending on the stressor (e.g., starvation, predation, and environmental change), life cycle events (e.g., migration and aging), and differ among vertebrate lineages, which react differently according to their biological, social and cognitive complexity (e.g., mineral demands, physical, and psychological stress). This review intends to summarize the endogenous GCs action on bone metabolism of mammals and fish under a variety of challenging circumstances. Particular emphasis will be given to the regulatory loop between GCs and the parathyroid hormone (PTH) family peptides, and other key regulators of mineral homeostasis and bone remodeling in vertebrates.

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Environmental and cortisol-mediated control of Ca2+ uptake in tilapia (Oreochromis mossambicus)

Cited by 23 publications

References 47 publications

Osmoregulation in freshwaters: Gene expression in the gills of a Neotropical cichlid in contrasting pH and ionic environments

Osmoregulation in freshwaters: Gene expression in the gills of a Neotropical cichlid in contrasting pH and ionic environments

Comparison of Calcium Balancing Strategies During Hypothermic Acclimation of Tilapia (Oreochromis mossambicus) and Goldfish (Carassius auratus)

Stress, Glucocorticoids and Bone: A Review From Mammals and Fish

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