Environmental exposure to the plasticizer 1,2-cyclohexane dicarboxylic acid, diisononyl ester (DINCH) in US adults (2000—2012)

Silva, Manori J.; Jia, Tao; Samandar, Ella; Preau, James L.; Calafat, Antònia M.

doi:10.1016/j.envres.2013.05.007

Cited by 131 publications

(91 citation statements)

References 15 publications

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“…Overall, the body burden of DINCH metabolites exhibited a similar time trend as previously discussed for the dust concentrations observed in German residences by Nagorka et al (2011a, b). In a third study, Silva et al (2013) collected anonymous urine samples in Atlanta in 2000, 2001, 2007, 2009, 2011, and The "typical" and "high" total DI and daily inhaled and ingested (house dust) amounts calculated from our study"s results were compared with estimated tolerable daily intake (TDI)…”

Section: Discussionsupporting

confidence: 52%

“…Our results and previously published data are summarized in Table 6. To date, DINCH metabolites have been measured only in three studies using adults (Schütze et al, 2012;2014;Silva et al, 2013). After developing an analytical method to quantify DINCH metabolites in urine, Schütze et al (2012) 1999, 2003, 2006, 2009, and 2012 were investigated to observe possible time trends for DINCH exposure since its market introduction (Schütze et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Cyclohexane-1,2-dicarboxylic acid was identified as the major but unspecific urinary metabolite, representing a 23.7% mean of the given dose of the three volunteers, while 10.7% was excreted after oxidation as OH-MICH, 2.0% as oxo-MINCH (1.5-2.6%), and 2.0% as cx-MINCH. The results of three general human population biomonitoring studies are currently available, allowing us to characterize the total exposure of DINCH (Schütze et al, 2012(Schütze et al, , 2014Silva et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Non-phthalate plasticizers in German daycare centers and human biomonitoring of DINCH metabolites in children attending the centers (LUPE 3)

Fromme

Schütze

Lahrz³

et al. 2016

International Journal of Hygiene and Environmental Health

114

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Non-phthalate plasticizers in German daycare centers and human biomonitoring of DINCH metabolites in children attending the centers (LUPE 3)

Fromme

Schütze

Lahrz³

et al. 2016

International Journal of Hygiene and Environmental Health

114

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“…Urine samples were vortexed, divided into several aliquots and stored at −80°C before being shipped overnight on dry ice to the CDC's National Center for Environmental Health, where the urinary biomarkers were quantified via enzymatic deconjugation, solidphase extraction, separation and analyses using liquid chromatography mass spectrometry (Silva et al, 2013).…”

Section: Urinary Biomarkers Measurementmentioning

confidence: 99%

Parental contributions to early embryo development: influences of urinary phthalate and phthalate alternatives among couples undergoing IVF treatment

Ashcraft

Whitcomb

et al. 2016

Hum. Reprod.

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STUDY QUESTION: Are preconception urinary concentrations of phthalates and phthalate alternatives associated with diminished early stage embryo quality in couples undergoing IVF?SUMMARY ANSWER: Male, but not female, urinary concentrations of select metabolites of phthalates and phthalate alternatives are associated with diminished blastocyst quality.WHAT IS KNOWN ALREADY: Although phthalates are endocrine disrupting compounds associated with adverse reproductive health, they are in widespread use across the world. Male and female preconception exposures to select phthalates have been previously associated with adverse reproductive outcomes in both the general population and in those undergoing IVF. STUDY DESIGN, SIZE, DURATION: This prospective cohort included 50 subfertile couples undergoing IVF in western Massachusetts. PARTICIPANTS/MATERIALS, SETTING, METHODS:This study includes the first 50 couples recruited from the Baystate Medical Center's Fertility Center in Springfield, MA, as part of the Sperm Environmental Epigenetics and Development Study (SEEDS). Relevant data from both partners, including embryo quality at the cleavage (Day 3) and blastocyst (Day 5) stages, were collected by clinic personnel during the normal course of an IVF cycle. A spot urine sample was collected from both male and female partners on the same day as semen sample procurement and oocyte retrieval. Concentrations of 17 urinary metabolite were quantified by liquid chromatography mass spectrometry and normalized via specific gravity. Generalized estimating equations were used to estimate odds ratios (OR) and 95% CI, with urinary phthalates and phthalate alternatives fitted as continuous variables and embryo quality as a binary variable. MAIN RESULTS AND THE ROLE OF CHANCE:The 50 couples contributed 761 oocytes, of which 423 progressed to the cleavage stage, 261 were high-quality cleavage stage embryos, 137 were transferrable quality blastocysts and 47 were high-quality blastocysts. At the cleavage stage, male urinary monoethyl phthalate concentrations were positively associated with high-quality cleavage stage embryos (OR = 1.20, 95% CI 1.01-1.43, P = 0.04); no other significant associations were observed at this stage. At the blastocyst stage, male urinary concentrations of monobenzyl phthalate (OR = 0.55, 95% CI 0.36-0.84, P = 0.01), mono-3-hydroxybutyl phthalate (OR = 0.37, 95% CI 0.18-0.76, P = 0.01), mono-n-butyl phthalate (OR = 0.55, 95% CI 0.42-0.73, P < 0.01) and monomethyl phthalate (OR = 0.39, 95% CI 0.26-0.60, P < 0.01) were inversely associated with high-quality blastocysts. A borderline statistically significant relationship was observed for male concentrations of mono (2-ethylhexyl) phthalate (OR = 0.52, 95% CI 0.27-1.00, P = 0.05) and cyclohexane-1,2-dicarboxylic acid-monocarboxy isooctyl ester (OR = 0.21, 95% CI 0.04-1.03, P = 0.05) at the blastocyst stage. Similar inverse associations were observed between male urinary phthalate metabolite concentrations and likelihood of transferrable quality blastocysts. For femal...

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“…We previously observed similar metabolite clusters for other plasticizers, including di-isononyl phthalate and 1,2-cyclohexane dicarboxylic acid, diisononyl ester. 15–18 Because all coeluting isomers of MEHHA and MEOHA are valid biomarkers of exposure to DEHA and similar in structure with different oxidation sites, we did not attempt to separate the isomers and included them together to facilitate their detection.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Metabolites of Di-2-ethylhexyl Adipate (DEHA) as Biomarkers of Exposure in Human Biomonitoring Applications

Silva

Samandar

et al. 2013

Chem. Res. Toxicol.

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Di-2-ethylhexyl adipate (DEHA) is a common plasticizer used in food packaging. At high doses, DEHA can cause adverse health effects in rats. Although the potential for human exposure to DEHA is high, no DEHA specific biomarkers are identified for human biomonitoring. Using human liver microsomes, we investigated the in vitro phase I metabolism of DEHA and its hydrolytic metabolite mono-2-ethylhexyl adipate (MEHA) and, for comparison purposes, of the analogous di-2-ethylhexyl phthalate (DEHP) and its hydrolytic metabolite mono-2-ethylhexyl phthalate. We unequivocally identified MEHA, a DEHA specific biomarker, and adipic acid, a nonspecific biomarker, using authentic standards. On the basis of their mass spectrometric fragmentation patterns, we tentatively identified two other DEHA specific metabolites: mono-2-ethylhydroxyhexyl adipate (MEHHA) and mono-2-ethyloxohexyl adipate (MEOHA), analogous to the oxidative metabolites of DEHP. Interestingly, although adipic acid was the major in vitro metabolite of DEHA, the analogous phthalic acid was not the major in vitro metabolite of DEHP. Our preliminary data for 144 adults with no known exposure to DEHA suggests that adipic acid is also the main in vivo urinary metabolite, while MEHA, MEHHA, and MEOHA are only minor metabolites. Therefore, the use of these specific metabolites for assessing the exposure of DEHA may be limited to highly exposed populations.

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Environmental exposure to the plasticizer 1,2-cyclohexane dicarboxylic acid, diisononyl ester (DINCH) in US adults (2000—2012)

Cited by 131 publications

References 15 publications

Non-phthalate plasticizers in German daycare centers and human biomonitoring of DINCH metabolites in children attending the centers (LUPE 3)

Non-phthalate plasticizers in German daycare centers and human biomonitoring of DINCH metabolites in children attending the centers (LUPE 3)

Parental contributions to early embryo development: influences of urinary phthalate and phthalate alternatives among couples undergoing IVF treatment

In Vitro Metabolites of Di-2-ethylhexyl Adipate (DEHA) as Biomarkers of Exposure in Human Biomonitoring Applications

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