Environmental immunoassay for the explosive RDX using a fluorescent dye-labeled antigen and the continuous-flow immunosensor

Bart, John C.; Judd, Linda L.; Kusterbeck, Anne W.

doi:10.1016/s0925-4005(97)80244-4

Cited by 32 publications

(20 citation statements)

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“…To assist in explosives detection for airport security and environmental remediation efforts, a biosensor has been developed at the Naval Research Laboratory based on immunoassays for explosives which are inexpensive, sensitive, and specific for the compound in question [93]. Recently, an analog and antigen-protein complex of the plastic explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) became commercially available.…”

Section: Fluorescencementioning

confidence: 99%

Recent Development in Optical Chemical Sensors Coupling with Flow Injection Analysis

Ojeda

Rojas

2006

Sensors

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Abstract:Optical techniques for chemical analysis are well established and sensors based on these techniques are now attracting considerable attention because of their importance in applications such as environmental monitoring, biomedical sensing, and industrial process control. On the other hand, flow injection analysis (FIA) is advisable for the rapid analysis of microliter volume samples and can be interfaced directly to the chemical process. The FIA has become a widespread automatic analytical method for more reasons; mainly due to the simplicity and low cost of the setups, their versatility, and ease of assembling. In this paper, an overview of flow injection determinations by using optical chemical sensors is provided, and instrumentation, sensor design, and applications are discussed. This work summarizes the most relevant manuscripts from 1980 to date referred to analysis using optical chemical sensors in FIA.

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Section: Fluorescencementioning

confidence: 99%

Recent Development in Optical Chemical Sensors Coupling with Flow Injection Analysis

Ojeda

Rojas

2006

Sensors

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“…Different optical [18,19] or voltammetric [20] sensors for RDX were also reported. These included the fluorescence detection of RDX with an acridinium dye, [18] or the application of NADH-functionalized quantum dots.…”

mentioning

confidence: 99%

Imprinted Au‐Nanoparticle Composites for the Ultrasensitive Surface Plasmon Resonance Detection of Hexahydro‐1,3,5‐trinitro‐1,3,5‐triazine (RDX)

2010

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The analysis of explosives attracts recent research efforts due to homeland security needs and the broad demand for the clearance of minefields. While numerous studies have addressed the development of sensing platforms for nitroaromatic explosives, and specifically trinitrotoluene (TNT), the detection of more hazardous explosives, such as hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) or pentaerythritol tetranitrate (PETN), is less developed and needs further efforts, particularly the improvement of the sensitivities associated with the analyses of these substrates.[1,2] Different optical, electrochemical, or microgravimetric sensors or biosensors for TNT were reported. Fluorescent organic polymers, which are quenched by nitroaromatic explosives, [3,4] luminescent polysilole nanoparticles (NPs), [5,6] or fluorescent silicon NPs quenched by nitroaromatic vapors enabled the development of optical sensors. The electrochemical activity of the nitro groups of TNT provided the basis for developing voltammetric sensors for this explosive, [7,8] and recently, a composite of Au NPs linked to electrodes enabled a sensitive electrochemical detection of TNT.[9] Also, different sensing matrices, such as cyclodextrin polymers, [10] carbowax, [11] or silicon polymers, [12] were used for TNT analysis by surface acoustic wave devices, and the aggregation of functionalized Au NPs in the presence of TNT was used to develop an optical sensor for the explosive.[13] Similarly, antibody-based optical [14][15][16] or microgravimetric quartz-crystal-microbalance [17] biosensors for TNT were developed. Different optical [18,19] or voltammetric [20] sensors for RDX were also reported. These included the fluorescence detection of RDX with an acridinium dye, [18] or the application of NADH-functionalized quantum dots.[21] Also, a competitive fluorescence immunoassay for the detection of RDX was reported.[22] The sensitivities accomplished by these methods are, however, unsatisfactory for analyzing trace amounts of the RDX explosive.Surface plasmon resonance (SPR) is a versatile method for probing changes in the refractive index occurring on thin metal films as a result of recognition events or chemical reactions. [23,24] Numerous SPR sensors and biosensors were developed, [25][26][27] and metal NPs were implemented to enhance the SPR response and to amplify SPR-based sensors. [28,29] The electronic coupling between the localized plasmon of the metallic NPs (e.g., Au NPs) and the surface plasmon wave enhances the SPR response and, thus, the labeling of recognition complexes with metallic NPs amplifies the sensing events. Different biosensing processes, such as DNA hybridization, [30] formation of immunocomplexes, [31] and the probing of biocatalytic transformations, [32] used Au NPs as labels for amplified SPR analyses. Recently, composites of bisaniline-crosslinked Au NPs were electropolymerized on Au electrodes, and the resulting matrices were used for the ultrasensitive SPR detection of TNT.[33] The formation of p-donor-acceptor complexes ...

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“…Fluorescent analogs of TNT and RDX were prepared as previously described [22,23,24]. Explosive standards were from Radian International (Austin, TX, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Tresyl chloride-activated Sepharose 4B (Sigma) or Emphaze AB1-activated chromatography beads (3M; from Pierce, Rockford IL, USA) were coupled to monoclonal antibodies against either TNT or RDX according to the manufacturer's standard procedure [22,24] to form the matrix for the mini-column used in the initial studies. The coupled antibody binding sites were saturated with a fluorescently-labeled analog of TNT for at least 7 days.…”

Section: Preparation Of Antibody-coated Substratesmentioning

confidence: 99%

Non-aerosol detection of explosives with a continuous flow immunosensor

Shriver‐Lake

Charles

Kusterbeck

2003

Analytical and Bioanalytical Chemistry

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Contamination of groundwater, soil, and the marine environment by explosives is a global issue. Identification, characterization and remediation are all required for a site recognized as contaminated with 2,4,6-trinitrotoluene (TNT) or hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). For each step, a method to accurately measure the contaminant level is needed. This paper reviews some of the current methods with emphasis on a single biosensor developed in our laboratory. Current regulatory methods require samples to be sent off-site to a certified laboratory resulting in time delays up to a month. A continuous flow biosensor for detection of explosives has been developed and tested for the rapid field screening of environmental samples. The detection system is based on a displacement immunoassay in which monoclonal antibodies to (TNT) and RDX are immobilized on solid substrates, allowed to bind fluorescently labeled antigens, and then exposed to explosives in aqueous samples. Explosive compounds present in the sample displace proportional amounts of the fluorescent label, which can then be measured to determine the original TNT or RDX concentration. The system can accurately detect ppb to ppt levels of explosives in groundwater or seawater samples and in extracts of contaminated soil. The biosensor has applications in environmental monitoring at remediation sites or in the location of underwater unexploded ordnance.

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Environmental immunoassay for the explosive RDX using a fluorescent dye-labeled antigen and the continuous-flow immunosensor

Cited by 32 publications

References 14 publications

Recent Development in Optical Chemical Sensors Coupling with Flow Injection Analysis

Recent Development in Optical Chemical Sensors Coupling with Flow Injection Analysis

Imprinted Au‐Nanoparticle Composites for the Ultrasensitive Surface Plasmon Resonance Detection of Hexahydro‐1,3,5‐trinitro‐1,3,5‐triazine (RDX)

Non-aerosol detection of explosives with a continuous flow immunosensor

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