Environmental immunoassays

Schneider, R.

doi:10.1007/s00216-002-1659-2

Cited by 28 publications

(22 citation statements)

References 18 publications

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“…Besides traditional enzyme immunoassay, FPIA is an on-going trend in environmental analysis [27] which offers the advantages of speed, simplicity and reproducibility of analysis [28]. The sensitivity reached by FPIA for NP developed in this work is sufficient for the preliminary screening of industrial wastewater effluents, where NP concentration can reach mg L -1 or even g L -1 values.…”

Section: Discussionmentioning

confidence: 96%

Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

et al. 2003

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The development of express method for detection of endocrine-disrupting chemicals (EDC) such as alkylphenols is required for ecological monitoring. Several attempts have been made to produce antibodies against 4-nonylphenol (NP) in recent years. This work describes the production of new antibodies against NP and also summarizes the characterization of antibodies obtained earlier. Three approaches used to produce alkylphenol-specific antibodies are compared; these are based on: 1. omega-(4-hydroxyphenyl)nonanoic or omega-(4-hydroxyphenyl)heptanoic acid NP derivatives designed to mimic the linear NP isomer; 2. 4-aminophenol, which potentially mimics various substituted phenolic compounds with different side-chain structures at position 4 of the benzene ring; and 3. a mixture of branched NP isomers, conjugated to the carrier protein via a benzene ring by the Mannich reaction, and expected to be the closest mimic of NP structure by preserving its natural alkyl moiety.Fluorescence polarization immunoassays based on different combinations of antibody and labeled antigen for screening detection of NP were developed and structural aspects of assay sensitivity and specificity were investigated. The assays based on the antisera raised against omega-(4-hydroxyphenyl)nonanoic acid and NP conjugate via Mannich reaction are capable of express detection of NP with detection limit of 7 microg mL(-1 )and assay dynamic range of 18-300 microg mL(-1).

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Section: Discussionmentioning

confidence: 96%

Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

et al. 2003

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“…Immunoassays have been growing in popularity and acceptance because of the reduction in the requirement for extensive sample preparation [5,6,7]. In some cases, such as for the detection of the explosive trinitrotoluene (TNT) in contaminated soil and ground water samples, on-site immunologically-based methods, such as colorimetric ELISAs [8,9] and biosensors [10,11], have been used.…”

Section: Introductionmentioning

confidence: 99%

Array biosensor for detection of toxins

Ligler

Taitt

Shriver‐Lake

et al. 2003

Analytical and Bioanalytical Chemistry

270

133

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The array biosensor is capable of detecting multiple targets rapidly and simultaneously on the surface of a single waveguide. Sandwich and competitive fluoroimmunoassays have been developed to detect high and low molecular weight toxins, respectively, in complex samples. Recognition molecules (usually antibodies) were first immobilized in specific locations on the waveguide and the resultant patterned array was used to interrogate up to 12 different samples for the presence of multiple different analytes. Upon binding of a fluorescent analyte or fluorescent immunocomplex, the pattern of fluorescent spots was detected using a CCD camera. Automated image analysis was used to determine a mean fluorescence value for each assay spot and to subtract the local background signal. The location of the spot and its mean fluorescence value were used to determine the toxin identity and concentration. Toxins were measured in clinical fluids, environmental samples and foods, with minimal sample preparation. Results are shown for rapid analyses of staphylococcal enterotoxin B, ricin, cholera toxin, botulinum toxoids, trinitrotoluene, and the mycotoxin fumonisin. Toxins were detected at levels as low as 0.5 ng mL(-1).

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“…Thus, the theory of ELISA and the principles of its development and optimization were considered in Russian reviews [24][25][26][27]; the use of ELISA for determining pesticides was also discussed in [28][29][30][31]. The fundamentals of ELISA and trends in the development of this technique for determining pesticides were surveyed by foreign authors [32][33][34][35]; procedures for the ELISA determination of pesticides in environmental samples [36][37][38] and food products [2,39,40] were also considered.…”

Section: Determination Methods For Pesticidesmentioning

confidence: 99%