Environmental influences on quality features of<i>Oviductus Ranae</i>in the Changbai Mountains

Xiao, Yao; Ni, Shuling; Wang, Shihan; Gan, Yuanshuai; Zhou, Yan; Guo, Hongye; Liu, Min; Wang, Zhihan; Wang, Yongsheng

doi:10.1039/c9ra04823e

Cited by 9 publications

(8 citation statements)

References 29 publications

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“…Oviductus Ranae , derived from dried tubal product of Rana temporaria chensinensis David, has the effects of anti-fatigue, nourishing the lungs and improving immunobiological activity [ 1 , 2 , 3 ]. As a precious natural product in northeast China, Oviductus Ranae has been used for nearly thousands of years and has developed into a series of nutrition, health food and traditional Chinese medicine.…”

Section: Introductionmentioning

confidence: 99%

“…The content of UFA is considered to be closely related to the quality of Oviductus Ranae [ 15 ]. However, due to the influence of breeding environment, production technology and preservation conditions [ 2 ], the content of UFA in Oviductus Ranae in different regions varies, resulting in the uneven quality of Oviductus Ranae in the market [ 15 ]. Therefore, it is necessary to develop an economical and convenient method to monitor the content of UFA in Oviductus Ranae to ensure its quality.…”

Section: Introductionmentioning

confidence: 99%

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Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

et al. 2021

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As one of the featured products in northeast China, Oviductus Ranae has been widely used as a nutritious food, which contains a variety of bioactive unsaturated fatty acids (UFAs). It is necessary to establish a scientific and reliable determination method of UFA contents in Oviductus Ranae. In this work, six principal UFAs in Oviductus Ranae, namely eicosapentaenoic acid (EPA), linolenic acid (ALA), docosahexaenoic acid (DHA), arachidonic acid (ARA), linoleic acid (LA) and oleic acid (OA), were identified using UPLC-MS/MS. The UFAs identified in Oviductus Ranae were further separated based on the optimized RP-HPLC conditions. Quantitative analysis of multi-components by single-marker (QAMS) method was implemented in content determination of EPA, ALA, DHA, ARA and OA, where LA was used as the internal standard. The experiments based on Taguchi design verified the robustness of the QAMS method on different HPLC instruments and chromatographic columns. The QAMS and external standard method (ESM) were used to calculate the UFA content of 15 batches of Oviductus Ranae samples from different regions. The relative error (r < 0.73%) and cosine coefficient showed that the two methods obtained similar contents, and the method validations met the requirements. The results showed that QAMS can comprehensively and effectively control the quality of UFAs in Oviductus Ranae which provides new ideas and solutions for studying the active components in Oviductus Ranae.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

et al. 2021

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“…Oviductus Ranae is the dried oviduct of female Rana temporaria chensinensis David, which is mainly produced in Changbai Mountain area, Jilin Province, China [ 17 , 18 , 19 ]. As a nutritional and health supplement for both medicine and food, Oviductus Ranae is rich in protein, sterol, unsaturated fatty acid and other nutrients [ 20 , 21 , 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity Evaluation of Oviductus Ranae Protein Hydrolyzed by Different Proteases

et al. 2021

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“…In the Rana chensinensis industry, Rana chensinensis ovum (RCO) is the residual by-product in the production process of Oviductus Ranae ( Figure 1 ) [ 1 , 2 , 3 , 4 , 5 , 6 ]. Due to the relatively small amount of research on the by-products, RCO is usually treated as waste, causing serious wastage of Rana chensinensis resources.…”

Section: Introductionmentioning

confidence: 99%

Rana chensinensis Ovum Oil Based on CO2 Supercritical Fluid Extraction: Response Surface Methodology Optimization and Unsaturated Fatty Acid Ingredient Analysis

Gan

Zhang

et al. 2020

Molecules

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Rana chensinensis ovum oil (RCOO) is an emerging source of unsaturated fatty acids (UFAs), but it is lacking in green and efficient extraction methods. In this work, using the response surface strategy, we developed a green and efficient CO2 supercritical fluid extraction (CO2-SFE) technology for RCOO. The response surface methodology (RSM), based on the Box–Behnken Design (BBD), was used to investigate the influence of four independent factors (pressure, flow, temperature, and time) on the yield of RCOO in the CO2-SFE process, and UPLC-ESI-Q-TOP-MS and HPLC were used to identify and analyze the principal UFA components of RCOO. According to the BBD response surface model, the optimal CO2-SFE condition of RCOO was pressure 29 MPa, flow 82 L/h, temperature 50 °C, and time 132 min, and the corresponding predicted optimal yield was 13.61%. The actual optimal yield obtained from the model verification was 13.29 ± 0.37%, and the average error with the predicted value was 0.38 ± 0.27%. The six principal UFAs identified in RCOO included eicosapentaenoic acid (EPA), α-linolenic acid (ALA), docosahexaenoic acid (DHA), arachidonic acid (ARA), linoleic acid (LA), and oleic acid (OA), which were important biologically active ingredients in RCOO. Pearson correlation analysis showed that the yield of these UFAs was closely related to the yield of RCOO (the correlation coefficients were greater than 0.9). Therefore, under optimal conditions, the yield of RCOO and principal UFAs always reached the optimal value at the same time. Based on the above results, this work realized the optimization of CO2-SFE green extraction process and the confirmation of principal bioactive ingredients of the extract, which laid a foundation for the green production of RCOO.

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Environmental influences on quality features ofOviductus Ranaein the Changbai Mountains

Abstract: This work studied the influences of environmental factors on the quality features of Oviductus Ranae.

Cited by 9 publications

References 29 publications

Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

Antioxidant Activity Evaluation of Oviductus Ranae Protein Hydrolyzed by Different Proteases

Rana chensinensis Ovum Oil Based on CO2 Supercritical Fluid Extraction: Response Surface Methodology Optimization and Unsaturated Fatty Acid Ingredient Analysis

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