Environmental Monitoring: Use of Luminescent Bacteria

Isenberg, Don L.; Bulich, Anthony A.

doi:10.1002/9783527616039.ch14

Cited by 6 publications

(2 citation statements)

References 19 publications

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“…Antagonistic and synergistic interactions with other compounds are complicating factors that can dramatically affect toxic responses both inside and outside the cell and within the cell membranes. (Bulich, Isenberg, 1981;Gitelson et al, 1984;Isenberg, Bulich, 1994;Kaiser et al, 1998;Wolska, Polkowska, 2001;Ulitzur et al, 2002;Wang et al, 2002;Parvez et al, 2006;Grande et al, 2007;Girotti et al, 2008a;Zhu et al, 2009 (50 % quenching luminescence) fully correlates with the LD 450 for the higher animals (Ganshin et al, 2001). Moreover, it becomes unnecessary to perform costly chemical analysis of all samples.…”

Section: Permanent Light Emission Of Luminescentmentioning

confidence: 99%

Bioluminescent Bioassays Based on Luminous Bacteria

K.¹,

M.²,

A.³

et al. 2009

J. Sib. Fed. Univ., Biol.

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Bioluminescent analysis is one of the most promising express methods for biologically monitoring the environment because the luminescent system is highly sensitive to even micro quantities of pollutants. Bioassays based on luminous bacteria give an integral estimation of toxicity and frequently surpass other known bioassays in speed, accuracy, sensitivity and simplicity. The enzymes of bacterial luminescent system are also used in developing highly sensitive analytical methods for practical purposes. This paper considers the main features of bacterial bioluminescence and isolated luminescent system, and also the applications of bioluminescence. One part reviews the investigations on the influence of different chemical substances on bacterial luminescence, development and using of bioluminescent bioassays, in particular some of them, in environmental monitoring of several regions in Russia. Luminous bacteria strains from the Culture Collection of Institute of Biophysics SB RAS (CCIBSO 836) are successfully used to produce new or improved bioassays based on lyophilized bacteria bearing the lux gene. Such bioassays are suitable for biotesting of water, air, soil, and the chemical substances used in everyday life. The «know-how» of kits for bioluminescent analysis and bioassays based on lyophilized natural luminous bacteria P.phosphoreum and recombinant Е.соli strain with cloned lux genes is developed. The bioluminescent assay has certificate and is recommended as an additional method of ecological monitoring in parallel with the other bioassays. The sensitivity of the bioassays developed in the IBP SB RAS is comparable to that of the foreign analogues Microtox ©TM , ToxAlert ©TM , etc.

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Section: Permanent Light Emission Of Luminescentmentioning

confidence: 99%

Bioluminescent Bioassays Based on Luminous Bacteria

K.¹,

M.²,

A.³

et al. 2009

J. Sib. Fed. Univ., Biol.

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“…Therefore, it is important to periodically monitor toxicants in the environment [ 3 ]. A simple and fast procedure is required to screen for the presence of toxic substances from industrial effluents, polluted rivers, and other polluted locations [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Microtox and Xenoassay Light as a Near Real Time River Monitoring Assay for Heavy Metals

Halmi

Jirangon

Johari

et al. 2014

The Scientific World Journal

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Luminescence-based assays for toxicants such as Microtox, ToxAlert, and Biotox have been used extensively worldwide. However, the use of these assays in near real time conditions is limited due to nonoptimal assay temperature for the tropical climate. An isolate that exhibits a high luminescence activity in a broad range of temperatures was successfully isolated from the mackerel, Rastrelliger kanagurta. This isolate was tentatively identified as Photobacterium sp. strain MIE, based on partial 16S rDNA molecular phylogeny. Optimum conditions that support high bioluminescence activity occurred between 24 and 30°C, with pH 5.5 to 7.5, 10 to 20 g/L of sodium chloride, 30 to 50 g/L of tryptone, and 4 g/L of glycerol as the carbon source. Assessment of near real time capability of this bacterial system, Xenoassay light to monitor heavy metals from a contaminated river running through the Juru River Basin shows near real time capability with assaying time of less than 30 minutes per samples. Samples returned to the lab were tested with a standard Microtox assay using Vibrio fishceri. Similar results were obtained to Xenoassay light that show temporal variation of copper concentration. Thus, this strain is suitable for near real time river monitoring of toxicants especially in the tropics.

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