The article deals with current environmental components of the problem of development of the territory (region) as a modern ecosystem. The peculiarities of forming the ecological potential of the territory are characterized. Special attention is paid to the research of terminological problems and the applied conceptual apparatus. Methodological principles providing formation of integral, logically verified methodology of assessment of ecological potential of the territory in modern conditions of development were formulated. The author’s approach to developing methodological bases for assessing the environmental situation in the territory is described.