Environmental rearing conditions are key determinants of changes in immune gene expression patterns in shrimp midgut

Pilotto, Mariana Rangel; Argenta, Nicolas; Forte, Jamille Martins; Hostins, Bárbara; Menezes, Francisca Gleire Rodrigues de; Maggioni, Rodrigo; Sousa, Oscarina Viana de; Wasielesky, Wilson; Rosa, Rafael Diego; Perazzolo, Luciane Maria

doi:10.1016/j.dci.2020.103618

Cited by 11 publications

(2 citation statements)

References 39 publications

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“…En el caso del camarón, se considera que el efecto inmunoestimulante de la BFT contribuye a mantener altos niveles de supervivencia bajo infecciones virales y bacterianas [20]. Algunas de las bacterias presentes en los bioflóculos aportan lipopolisacáridos y peptidoglicanos, ayudando a activar la inmunidad innata de los peces [16].…”

Section: Contribución De Los Bioflóculos a La Inmunidad De Los Organi...unclassified

Tecnología de Bioflóculos: Un camino hacia la acuacultura sustentable

Ochoa-Hernández,

Villanueva-Gutiérrez,

Martínez-Córdova

et al. 2023

Epistemus

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La acuacultura se ha convertido en el sector alimentario con la más alta tasa de crecimientos en los últimos años. Sin embargo, esta actividad tiene un importante impacto ambiental principalmente por el uso de grandes cantidades de agua y la alta cantidad de efluentes contaminados. La tecnología de bioflóculos (BFT) ofrece una alternativa sustentable que, a través de la actividad metabólica de una comunidad de microorganismos, puede ayudar a mantener la calidad del agua, permite ser utilizada como alimento por los organismos de cultivo y beneficia el sistema inmune de los organismos acuáticos. En este artículo se presenta a la BFT como una potencial herramienta que permita obtener alimentos de calidad y dirija la acuacultura hacia un camino sustentable

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Section: Contribución De Los Bioflóculos a La Inmunidad De Los Organi...unclassified

Tecnología de Bioflóculos: Un camino hacia la acuacultura sustentable

Ochoa-Hernández,

Villanueva-Gutiérrez,

Martínez-Córdova

et al. 2023

Epistemus

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“…The midgut of shrimp consists of the outer epidermis, muscular layers, protruding mucosal folds, and the inner epidermis, and an intact intestinal structure forms the basis for the functioning of the intestine (Xie et al, 2020). In addition, the immune function of the gut should be considered, as pathogens in the external environment can pass through the intestine (Pilotto et al, 2020). The antimicrobial peptides such as alf (antilipopolysaccharide factor), cru (crustins), and pen3 (penaeidins 3) are regulated by secreted cytokines and immune signaling pathways, such as the Toll and IMD (immune deficiency).…”

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confidence: 99%

Evaluation of the Dietary Black Soldier Fly Larvae Meal (Hermetia illucens) on Growth Performance, Intestinal Health, and Disease Resistance to Vibrio parahaemolyticus of the Pacific White Shrimp (Litopenaeus vannamei)

et al. 2021

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The present study evaluated the effects of the dietary black soldier fly larvae meal (BSF) on growth performance, intestinal health, and susceptibility to Vibrio parahaemolyticus in the Pacific white shrimp Litopenaeus vannamei. The basal diet was formulated to contain 25% fish meal (FM), and then the FM was replaced with BSF for 10, 20, and 30% of the FM protein in the experimental diets, which are referred to as FM, BSF10, BSF20, and BSF30, respectively. Four hundred and eighty healthy and uniform-sized shrimp (~0.88 g) were distributed among four groups of three replicates, each with 40 shrimp in a 300-L tank and they were fed four times daily for 7 weeks. The results showed that the growth performance did not change significantly in shrimp fed with BSF10 and BSF20 diets, but significantly decreased in those fed with BSF30 diet compared to the ones fed with FM diet. After feeding trial and sampling, a V. parahaemolyticus infection challenge trial was conducted on shrimp. The results showed that the survival rate of shrimp fed with BSF10 was significantly higher than those fed with FM. The results of the midgut histology showed that the width and height of intestinal mucosal folds decreased significantly in shrimp fed with BSF20 and BSF30, and the early signs of apoptosis in the intestinal cells were found in shrimp fed with BSF30. The mRNA levels of non-specific immune-related genes dorsal and relish were downregulated in shrimp fed with BSF20 and BSF30 diets. The mRNA levels of antimicrobial peptides–related genes alf (anti-lipopolysaccharide factor) were upregulated in shrimp fed with BSF10 but downregulated in shrimp fed with BSF30. The mRNA level pen3 (penaeidins 3) was upregulated in shrimp fed with BSF10 and BSF20 diets. The intestinal bacterial communities on operational taxonomic unit levels among groups were not significantly differentiated according to the beta diversity analysis. At the genus levels, a decrease in Vibrio, Photobacterium, and Candidatus_Bacilloplasma, as well as the increase in Bacillus and Pseudoalteromonas abundance, indicated the improvement of intestinal microbiota in shrimp fed with dietary BSF. Therefore, the use of BSF in shrimp diet should be controlled at a dosage of 20% of the FM, which can improve the intestinal microbiota without causing any negative effects.

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