Environmentally friendly nano-selenium to improve antioxidant system and growth of groundnut cultivars under sandy soil conditions

Hussein, Hebat-Allah A.; Darwesh, Osama M.; Mekki, B.B.

doi:10.1016/j.bcab.2019.101080

Cited by 106 publications

(87 citation statements)

References 29 publications

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“…There was no statistically significant difference in mean scores between Penicillium crustosum and Mucor circinelloides as shown in Table 2. These results were in concurrence with many authors [38,29,39] that those strains are from the most promising isolates for Lglutaminase activity.…”

Section: Effect Of Incubation Period On L-glutaminase Activitysupporting

confidence: 92%

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Evaluation of antitumor activity of fungal L-glutaminase produced by egyptian isolates

2020

Lett Appl NanoBioSci

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L-glutaminase is an amidohydrolase that catalyzes L-glutamine to L-glutamic acid and has increased impressive consideration due to its potential anticancer activity. The objective of this work was to isolate different fungi that produce L-glutaminase which has antitumor activities. Three fungal species that were isolated on the selective medium showed L-glutaminase activity. The species were identified as a Penicillium crustosum, Emericella nidulans and Mucor circinelloides. Mucor circinelloides showed potential L-glutaminase production (5.16 U/ml). Different pH, L-glutamine concentrations, temperatures, and incubation periods were carried out. The most enzyme production was shown at pH 8, temperature 30 ºC, with 0.6% of the L- glutamine. L-glutaminase was extracted and purified with gel filtration and ion exchange (DEAE Sephadex A50). The purified enzyme was found to possess a specific activity 1.55 U/mg and was monitored by SDS-PAGE with molecular weight 70 kDa. Purified L-glutaminase exhibited cytotoxic activity against HepG2 and PC3 cell lines with Ic50 value 0.067 mg/ml and 0.079 mg/ml respectively. Caspase 3 colorimetric assay and flow cytometry was done to detect the activation of apoptosis among the enzyme. The enzyme showed positive results on both types of cell lines.

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Section: Effect Of Incubation Period On L-glutaminase Activitysupporting

confidence: 92%

“…The data were analyzed by two -way analysis of variance (ANOVA) using IBM SPSS (25.0), followed by Duncan's multiple range test to compare the differences among treatments where significant differences (P < 0.05) were observed. [29].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of antitumor activity of fungal L-glutaminase produced by egyptian isolates

2020

Lett Appl NanoBioSci

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“…Behavior of the actinomycetes isolates toward different substrates of the selective enzymes was noted on agar medium. Pure isolates with maximum growth range (on agar plates) were cultured on basal mineral salts agar medium with addition of glucose for control, starch for amylase [49], casein for protease [50], tributyrin for lipase [51], carboxy methyl cellulose for cellulose [52] and pectin for pectinase [53].…”

Section: Screening For Enzymes Assay Of the Isolated Actinmomycetesmentioning

confidence: 99%

“…The enzyme activity was given as unit (U) and one unit is that hydrolyze casein to produce color equivalent to 1.0 µmole (181.0 µg) of tyrosine per min under the defined assay conditions. Lipase was tested using 1% tri butyrin as detailed by Hussein et al [51]. Also, cellulase was assayed using carboxy methyl cellulose as mentioned by Hasanin et al [52].…”

Section: Optimization and Examination Of Beneficial Enzymes Productionmentioning

confidence: 99%

Thermoflavimicrobium dichotomicum as a novel thermoalkaliphile for production of environmental and industrial enzymes

2019

Biointerface Res Appl Chem

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Thermoalkaliphilic actinomycetes enzymes have many important applications in many industrial, biotechnological and environmental aspects. So, the current study aimed to obtain the thermoalkali-enzymes producing actinomycetes. A novel thermoalkaliphilic actinomycete strain was isolated from Egyptian Siwa oasis and identified according to its morphological, physiological and biochemical characters as Thermoflavimicrobium dichotomicum. And then confirmed by phylogenetic analysis and the partial sequence was deposited in GenBank under accession number of KR011193 and name of Thermoflavimicrobium dichotomicum HwSw11. It could produce amylase, cellulase, lipase, pectinase and proteinase enzymes. Also, this strain exhibited anti-bacterial activities against P. aeruginosa and E. coli with inhibition zones of 14 and 20 mm, respectively. Consequently, it has antifungal activity against A. niger, A. flavus and Penicillium notatum with inhibition zones of 17, 14 and 14 mm, respectively. For that, it may be concluded that Thermoflavimicrobium dichotomicum HwSw11 as a novel thermoalkaliphile has a wide range of biological activities against a broad spectrum of pathogenic bacteria and fungi, in addition to produce many enzymes (amylase, cellulase, lipase, pectinase and proteinase). So, this isolate could be applied as manufactory for many industrial, biotechnological and environmental sectors.

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“…Selenium is essential to human health due to the fact of its role as a cofactor of enzymes related to antioxidant metabolism [12]; however, in plants, Se also exerts a positive effect on antioxidant capacity [13]. It has been shown that selenium nanoparticles (Se NPs) can function as stimulants in plants, improving their antioxidant defense system and, therefore, their ability to tolerate stress [14]. Selenium NPs also have excellent biological properties and low toxicity [15], unlike the bulk form that has a well-documented toxic effect (due to the oxidative stress) in plants at high concentrations [16].…”

Section: Introductionmentioning

confidence: 99%

Se Nanoparticles Induce Changes in the Growth, Antioxidant Responses, and Fruit Quality of Tomato Developed under NaCl Stress

et al. 2019

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Nanotechnology represents an opportunity to improve the use of elements in agriculture. Selenium is an element that is beneficial to plants and essential to the human diet. The size of nanoparticles gives them characteristics that can enhance the benefits that selenium provides to plants. The objective of the present study was to determine the effects of selenium nanoparticles on the growth, antioxidant responses, and fruit quality of tomato developed under NaCl stress. Four doses of selenium nanoparticles (1, 5, 10, and 20 mg L−1) under NaCl stress, only NaCl, and a control were evaluated. The results showed that the impact of salinity on the growth of the tomato crop can be reduced with the application of selenium nanoparticles. However, the amount of both enzymatic and non-enzymatic compounds significantly increased in the leaves and fruits of tomato. The results suggest that the application of selenium nanoparticles generated a positive effect against salinity in the tomato crop; moreover, it had a positive impact on the content of beneficial biocompounds for human health in tomato fruits.

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Environmentally friendly nano-selenium to improve antioxidant system and growth of groundnut cultivars under sandy soil conditions

Cited by 106 publications

References 29 publications

Evaluation of antitumor activity of fungal L-glutaminase produced by egyptian isolates

Evaluation of antitumor activity of fungal L-glutaminase produced by egyptian isolates

Thermoflavimicrobium dichotomicum as a novel thermoalkaliphile for production of environmental and industrial enzymes

Se Nanoparticles Induce Changes in the Growth, Antioxidant Responses, and Fruit Quality of Tomato Developed under NaCl Stress

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