Enzymatic and mechanically induced subretinal neovascularization

Abstract

Mechanical and enzymatic disruption of Bruch's membrane and induction of subretinal neovascularization in an experimental primate model have produced limited success. The methods and results are reported here to identify some problems encountered in methodology and to detail the results of this approach to subretinal neovascularization.

Correlation between clinical and histological features in a pig model of choroidal neovascularization

“…Bruch's membrane perforation alone has previously been shown not to be sufficient to elicit CNV [10]. With our approach CNV was successfully achieved in all four eyes studied.…”

A Subretinal Matrigel Rat Choroidal Neovascularization (CNV) Model and Inhibition of CNV and Associated Inflammation and Fibrosis by VEGF Trap

“…In contrast, selective damage to Bruch's membrane, either mechanical or enzymatic, has met with only limited success in inducing subretinal neovascularization in primates. 38 Subretinal delivery of adenovirus or adenoassociated virus carrying a VEGF transgene has been used successfully to induce subretinal neovascularization in rats. 39 -41 Subretinal injection of FGF2-impregnated gelatin microspheres also has been reported to induce CNV in more than 80% of injected eyes in rabbits, 42 and VEGF-impregnated gelatin microspheres induces CNV in more than 90% of injected eyes in primates.…”

A new animal model of choroidal neovascularization

“…The first systematic attempt to develop an animal model of CNV was performed in the rhesus monkey Macca mulatta using argon laser photocoagulation (Ryan 1979, 1980; Ryan et al 1980). Mechanical stress, enzymatic digestion of Bruch's membrane (BM) or the transchoroidal infusion of basic fibroblast growth factor (bFGF) have also been tried in the search for a suitable model of CNV (Hsu et al 1989; Soubrane et al 1994).…”