Enzymatic and structural characterization of new PLA2 isoform isolated from white venom of Crotalus durissus ruruima

Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from Crotalus oreganus lutosus (Great Basin rattlesnake) snake venom

“…Peptide fragment Molecular mass (Da) Accuracy (ppm) from viperid venoms (Bouchier et al, 1988;Chen et al, 2004;Damico et al, 2005;Diz Filho et al, 2009;Faure et al, 2011) injection of ColTx-I (5, 10 and 20 mg/animal). Controls were injected with 50 ml of PBS.…”

Phospholipases A2 Protein Structure and Natural Products Interactions in Development of New Pharmaceuticals

“…The phospholipase A2 (PLA2), EC 3.1.1.4, are enzymes that release fatty acids from the second carbon group of glycerol. These enzymes are common in many living organisms and can be found both intra and extracellularly, which are referred to as secretory PLA2 ( sPLA2) and cytosolic PLA2 (cPLA2), respectively (Burke & Dennis, 2009;Dennis, 1997;Diz Filho et al, 2009;Schaloske & Dennis, 2006). The enzymatic action of cPLA2 on lipids, allows the formation of a compound that acts as mediators of inflammatory diseases (Chakraborti, 2003;Dennis, 1994).…”

Neuromuscular effects of venoms and crotoxin-like proteins from Crotalus durissus ruruima and Crotalus durissus cumanensis

“…CTX was originally isolated from Crotalus durissus terrificus venom (Slotta and Fraenkel-Conrat, 1938) and, subsequently, proteins simile (CTX-like) have been found in venoms of several other subspecies of C. durissus (Beghini et al, 2000;Calvete et al, 2010;Diz Filho et al, 2009;Lennon and Kaiser, 1990;Pereañez et al, 2009;Ponce-Soto et al, 2007). Proteomic studies revealed that CTX comprises more than 50% of the toxin venoms from several C. durissus (C.d.)…”

“…In this study, we used the "white" venom of Cdr (Dos-Santos et al, 1993). CTXs were purified as previously described (Diz-Filho et al, 2009;Pereañez et al, 2009). Animal procedures were in accordance with the guidelines prepared by the Committee on Care and Use of Laboratory Animal Resources, National Research Council, USA.…”