Enzymatic Hydrogelation-Induced Fluorescence Turn-Off for Sensing Alkaline Phosphatase in Vitro and in Living Cells

Liu, Dong; Miao, Qingqing; Hai, Zijuan; Yuan, Yue; Liang, Gaolin

doi:10.1021/acs.analchem.5b01657

Cited by 140 publications

(70 citation statements)

References 27 publications

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“…Enhanced quenching has been employed by researchers to detect the activity of enzymes (ex. caspase 3 [79], alkaline phosphatase [80], etc.). However, self-assembly can also leads to a reduction of the charge transfer between the fluorophore and the polar solvent, and thus an increment of fluorescence [81].…”

Section: Peptide-peptide Interaction Driven Self-assemblymentioning

confidence: 99%

Self-assembling peptide-based building blocks in medical applications

Acar

Srivastava

Chung

et al. 2017

Advanced Drug Delivery Reviews

203

155

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Peptides and peptide-conjugates, comprising natural and synthetic building blocks, are an increasingly popular class of biomaterials. Self-assembled nanostructures based on peptides and peptide-conjugates offer advantages such as precise selectivity and multifunctionality that can address challenges and limitations in the clinic. In this review article, we discuss recent developments in the design and self-assembly of various nanomaterials based on peptides and peptide-conjugates for medical applications, and categorize them into two themes based on the driving forces of molecular self-assembly. First, we present the self-assembled nanostructures driven by the supramolecular interactions between the peptides, with or without the presence of conjugates. The studies where nanoassembly is driven by the interactions between the conjugates of peptide-conjugates are then presented. Particular emphasis is given to in vivo studies focusing on therapeutics, diagnostics, immune modulation and regenerative medicine, and challenges and future perspective are presented.

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Section: Peptide-peptide Interaction Driven Self-assemblymentioning

confidence: 99%

Self-assembling peptide-based building blocks in medical applications

Acar

Srivastava

Chung

et al. 2017

Advanced Drug Delivery Reviews

203

155

View full text Add to dashboard Cite

show abstract

“…Among these assays, fluorometric methods have attracted considerable interest for their rapid response, easy operation and high sensitivity 17–19 . Traditional fluorescent methods are mainly based on organic dyes 20, 21 , fluorescent polymers 22, 23 and metal nanoclusters or nanoparticles 24–26 . Whereas, most of them have poor photostability, laborious synthetic procedures, and complexed labeling processes.…”

Section: Introductionmentioning

confidence: 99%

Nitrogen-doped Carbon Dots Mediated Fluorescent on-off Assay for Rapid and Highly Sensitive Pyrophosphate and Alkaline Phosphatase Detection

Geng

Zhang

et al. 2017

Sci Rep

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In this report, a novel fluorescent sensing platform using nitrogen-doped carbon dots (N-CDs) as probes for fluorescence signal transmission has been designed for the detection of significant biomolecules pyrophosphate (PPi) and alkaline phosphatase (ALP). The high fluorescent N-CDs could be selectively quenched by Cu2+, and recovered by the addition of PPi because PPi preferentially binds to Cu2+. Once ALP was introduced into the system, ALP can specifically hydrolyze PPi into Pi, the intense fluorescence of N-CDs could be quenched again due to the recombination of the as-released Cu2+ with N-CDs. So, fluorescence of N-CDs is regulated by an ALP-triggered reaction. Based on this strategy, we demonstrated that N-CDs could serve as a very effective fluorescent sensing platform for label-free, sensitive and selective detection of PPi and ALP with low detection limit of 0.16 μM and 0.4 U/L for PPi and ALP, respectively. Moreover, the assay time is just around 0.5 min for PPi and 30 min for ALP. This developed strategy shows remarkable advantages including sensitive, rapid, simple, convenient, and low-cost and so forth. Furthermore, this method was also successfully applied to monitor ALP in human serum, which indicates its great potential for practical applications in biological and clinical diagnosis.

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“…Higher and lower activities of ALP are utilized as am arker for liver,b one, and hypophosphatasia-relatedd isorders. [83,84] Pyrophosphate (PPi)i sa natural substrate that inhibits the fluorescencea ctivity of dsDNA-templated CuNPs by forming ac omplex with Cu 2 + and terminating the growth of CuNPs.A LP hydrolyzes PPi into inorganic phosphate (Pi), which cannot form ac omplex with Cu 2 + .T he resultant fluorescencei ntensity of dsDNA-templated CuNPsr emains, as shown in Figure 4A.…”

Section: Copper Nps As Af Luorescencebiosensormentioning

confidence: 99%

Strategies for the Development of Metallic‐Nanoparticle‐Based Label‐Free Biosensors and Their Biomedical Applications

et al. 2019

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Label‐free biosensors offer accurate sensing capabilities due to the reliable quantification of biological and biochemical processes. These devices function by establishing a dynamic interaction of analyte and receptor molecules and convert this interaction into a measurable signal through a transducer. In recent decades, label‐free biosensors have attracted attention in biomedical applications due to the ease of linking nanomaterials with bioreceptor molecules. In this review, recent advances in sensitivity, specificity, and sensing mechanism related to label‐free biosensors of metallic nanoparticles of gold, silver, aluminium, copper, and zinc oxide are presented. Selected sensing methods based on fluorescence, surface plasmon resonance, surface‐enhanced Raman scattering, metal‐enhanced fluorescence, and electrochemical sensors are discussed. New measurement techniques and rapid progress of label‐free biosensors are going to play a vital role in the real‐time detection of biomarkers in clinical samples, such as blood plasma, serum, and urine, as well as in targeted drug delivery. Future trends of these label‐free biosensing mechanisms and their development are also discussed.

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Enzymatic Hydrogelation-Induced Fluorescence Turn-Off for Sensing Alkaline Phosphatase in Vitro and in Living Cells

Cited by 140 publications

References 27 publications

Self-assembling peptide-based building blocks in medical applications

Self-assembling peptide-based building blocks in medical applications

Nitrogen-doped Carbon Dots Mediated Fluorescent on-off Assay for Rapid and Highly Sensitive Pyrophosphate and Alkaline Phosphatase Detection

Strategies for the Development of Metallic‐Nanoparticle‐Based Label‐Free Biosensors and Their Biomedical Applications

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