Enzymatic iodination of polypeptides with 125I to high specific activity

Thorell, Jan I.; Johansson, Bengt

doi:10.1016/0005-2795(71)90123-1

Cited by 1,173 publications

(276 citation statements)

References 17 publications

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“…MC3-R-and MC4-R-expressing sublines were identified by screening 8 -12 colonies for specific binding of [ 125 I]-NDP-MSH. In cell sublines selected for additional study, the presence of functional MCR having agonist binding profiles and coupling to adenylate cyclase consistent with those reported previously Alvaro et al, 1996) (Sawyer et al, 1980) was prepared by modifications of methods published previously (Tatro and Reichlin, 1987;Tatro, 1993), using a standard method (Thorell and Johansson, 1971) for lactoperoxidase-catalyzed radioiodination. Briefly, 4 g of NDP-MSH in 100 l of 10 mM sodium phosphate, pH 6.5, was added to 10 l (1 mCi) of [ 125 I]-Na (Amersham, Arlington Heights, IL) and 5 l of lactoperoxidase (0.8 mg/ml) (Calbiochem, La Jolla, CA).…”

Section: Methodsmentioning

confidence: 70%

Antipyretic Role of Endogenous Melanocortins Mediated by Central Melanocortin Receptors during Endotoxin-Induced Fever

et al. 1997

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Bacterial infection causes fever, an adaptive but potentially self-destructive response, in the host. Also activated are counterregulatory systems such as the pituitary-adrenal axis. Antipyretic roles have also been postulated for certain endogenous central neuropeptides, including the melanocortins (␣-MSHrelated peptides). To test the hypothesis that endogenous cen- -9119 (168 pmol, i.c.v.) prevented the antipyretic action of exogenous ␣-MSH. In contrast, neither ␣-MSH nor SHU-9119, alone or in combination, affected body temperatures in afebrile rats. In LPS-treated rats, intracerebroventricular injection of SHU-9119 significantly increased fever, whereas intravenous injection of the same dose of SHU-9119 had no effect. Neither intracerebroventricular nor intravenous SHU-9119 significantly affected LPS-stimulated plasma ACTH or corticosterone levels. The results indicate that endogenous central melanocortins exert an antipyretic influence during fever by acting on MCRs located within the brain, independent of any modulation of the activity of the pituitary-adrenal axis.

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Section: Methodsmentioning

confidence: 70%

Antipyretic Role of Endogenous Melanocortins Mediated by Central Melanocortin Receptors during Endotoxin-Induced Fever

et al. 1997

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“…The purified eCG was radiolabeled using 125-Iodine (IMS-30; Amersham, Little Chalfont, UK) according to the enzymatic procedure of Thorell and Johansson (1971). The antibody levels were expressed as the percentage of 125 I-eCG bound by 10 ml of plasma.…”

Section: Antibody Detection In Plasma Samplesmentioning

confidence: 99%

Evolution of anti-eCG antibodies in response to eCG doses and number of injections. Relationship with productivity of rabbit does

Theau-Clément¹,

Lebas²,

Beckers

et al. 2008

Animal

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The aim of this experiment was to study the kinetics of anti-eCG (equine chorionic gonadotrophin) antibodies in relation to eCG dose (8 or 25 IU) and number of injections (n 5 11) in comparison with a control group (no injection), and to relate antibody production to sexual receptivity and productivity of rabbit does. In all, 124 lactating primiparous rabbit does were inseminated every 35 days for a year. Just before eCG injection (48 h before insemination), blood samples were collected from all the does to assay anti-eCG antibodies. The anti-eCG antibody binding rate, regardless of the injected dose, shows that none of the does developed detectable anti-eCG antibodies before the 7th injection. The level of detectable anti-eCG antibodies began to show an increase at the 7th injection and was significant only for the 25 IU dose at the 11th injection. At the end of the experiment, 15% and 39% of does treated with 8 and 25 IU, respectively, developed immunity to eCG (binding rate .6%: higher binding rate of the control group). Consequently, the immune response depends on the eCG dose and on the number of injections. Moreover, productivity of does estimated from the number of weaned rabbits produced per insemination is not influenced by the level of eCG antibodies (7.0 and 6.9 for binding rate ,6% and binding rate >6%, respectively). Only 19 inseminations (n 5 6 and n 5 13 for 8 and 25 IU, respectively) were made on hyperimmune does. Consequently, the immune response to eCG seems to be marginal for rabbit does. Moreover, under the described experimental conditions, reproductive performances of hyperimmune does were not affected.

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“…and used to prepare '25I EGF. A total of 19 iodinations were carried out using either iodogen (Fraker & Speck, 1978; n = 16) or lactoperoxidase (Thorell & Johnson, 1971; n = 3). The radioactive EGF thus prepared was stored in aliquots at -40°C until required, for up to 1 month.…”

Section: Radiochemicalsmentioning

confidence: 99%

Epidermal growth factor receptors in intracranial and breast tumours: their clinical significance

Hawkins

Killen²,

Whittle³

et al. 1991

Br J Cancer

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Summary A method to determine the binding of epidermal growth factor (EGF) to the particulate fraction of the cell has been established and evaluated using rat liver, human placenta, and tumours of human breast and brain. Little EGF receptor (EGFR) activity was detected in normal or benign tumour tissues except for meningioma (positive in 95% samples), but EGFR were present in 43% of 131 breast tumours and 75% of 55 primary cerebral tumours. Despite the strong inverse correlation between EGFR activity and oestrogen receptors in breast tumours and a tendency for high levels of EGFR activity to be associated with glioblastoma multiforme, analysis showed that EGFR was of little prognostic significance in patients with tumours of either breast or brain.

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Enzymatic iodination of polypeptides with 125I to high specific activity

Cited by 1,173 publications

References 17 publications

Antipyretic Role of Endogenous Melanocortins Mediated by Central Melanocortin Receptors during Endotoxin-Induced Fever

Antipyretic Role of Endogenous Melanocortins Mediated by Central Melanocortin Receptors during Endotoxin-Induced Fever

Evolution of anti-eCG antibodies in response to eCG doses and number of injections. Relationship with productivity of rabbit does

Epidermal growth factor receptors in intracranial and breast tumours: their clinical significance

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