Enzymatic methods in food analysis: determination of ascorbic acid

Шеховцова, Т. Н.; Muginova, S. V.; Luchinina, Julia A.; Galimova, Anna Z.

doi:10.1016/j.aca.2006.05.015

Cited by 55 publications

(26 citation statements)

References 27 publications

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“…[5] In food industry, AA is widely used as an antioxidant to avert the formation of carcinogenic nitrosamines in meat and sausage products. [6] The conventional detection methods for AA include titrimetric analysis, liquid chromatography [7,8], fluorometric assay [9], spectrophotometric determination, [10] and electrochemical techniques [11]. Titrimetric methods to AA analysis are simple, but usually need big volume of analyte solution.…”

Section: Introductionmentioning

confidence: 99%

Interference-blind microfluidic sensor for ascorbic acid determination by UV/vis spectroscopy

Fernandes

Cardoso

et al. 2016

Sensors and Actuators B: Chemical

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A microfluidic sensor is developed and targeted at specific ingredients determination in drug/food/beverage matrices. The surface of a serpentine polydimethylsiloxane (PDMS) microchannel is modified by enzyme via physisorption. When solutions containing target ingredients pass through the microfluidic channel, enzyme-catalyzed reaction occurs and only converts the target molecules to its products. The whole process is monitored by an end-channel UV/vis spectroscopic detection. Ascorbate oxidase and L-ascorbic acid (AA) are taken as enzyme-substrate model in this study to investigate the feasibility of using the developed strategy for direct quantification of AA in standard solutions and complex matrices. A dietary supplement product, vitamin C tablet, is chosen as a model matrix to test the microfluidic bio-sensor in real-sample analysis. The results illustrate that the established microfluidic biosensor exhibits good reproducibility, stability, and anti-interference property. Technically, it is easy to realize, depends on low investment in chip fabrication, and simple instrumental procedure, where only UV/vis spectrophotometer is required. To sum up, the developed strategy is economical, specific, and accurate, and can be potentially used for fast quantification of ingredient in samples with complex matrix background. It is promising to be widely spread in food industry and quality control department.

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Section: Introductionmentioning

confidence: 99%

Interference-blind microfluidic sensor for ascorbic acid determination by UV/vis spectroscopy

Fernandes

Cardoso

et al. 2016

Sensors and Actuators B: Chemical

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“…The direct proportionality be tween the length of the induction period and the concen tration of ascorbic acid underlies the procedure for its enzymatic determination. 26 Thus, the use of two different analytical signals, viz., the indicator reaction rate and the length of induction period, opens up the way for determination of organic …”

Section: The Use Of Various Analytical Signalsmentioning

confidence: 99%

Enzymatic methods of analysis: Novel approaches and applications

2007

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The results of a series of investigations dealing with the development of enzymatic methods for determination of biologically active compounds, viz., inhibitors, activators, and substrates of native and immobilized enzymes of the oxidoreductase (peroxidases, alcohol dehydro genases) and hydrolase (alkaline and acid phosphatases) classes isolated from diverse sources are summarized. Novel original approaches, proposed by the authors, for improving the sensi tivity, selectivity, and rapidity of the methods are discussed. Numerous examples of application of the developed enzymatic procedures for the analysis of a wide range of samples are given.

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“…Pharmacopoeial authorities still describe the iodimetric titration method [7,8] for the assay of AA substance. Enzymatic methods for the determination of AA are based on the action of plant peroxidases like peanut peroxidase [9], horseradish peroxidase [10] or ascorbate oxidase enzyme electrode [11]. Electrochemical methods for the determination of AA include voltammetry [12,13] and potentiometry [14].…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic interaction liquid chromatography method for the determination of ascorbic acid

Nováková¹,

Solichová

Pavlovičová

et al. 2008

J of Separation Science

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Hydrophilic interaction liquid chromatography (HILIC) method using internal standard for the determination and stability study of ascorbic acid was developed. HILIC method was very fast and simple using the following analytical conditions: ZIC HILIC (150 x 2.1 mm, 3.5 microm) chromatographic column and mobile phase composed of ACN and 50 mM ammonium acetate buffer pH 6.8 (78:22 v/v). Diode array detection was performed and chromatograms were processed at 268 nm, the maximum wavelength of absorbance of ascorbic acid. An extensive stability study of ascorbic acid as a function of various factors including temperature, stabilizing agents, oxygen presence and its concentration in solution was performed in order to gain information about the quantitative influence of individual stability factors. Low temperature and stabilizing agents (o-phosphoric acid and oxalic acid) were found to be key factors enabling substantial enhancement of the stability of ascorbic acid.

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Enzymatic methods in food analysis: determination of ascorbic acid

Cited by 55 publications

References 27 publications

Interference-blind microfluidic sensor for ascorbic acid determination by UV/vis spectroscopy

Interference-blind microfluidic sensor for ascorbic acid determination by UV/vis spectroscopy

Enzymatic methods of analysis: Novel approaches and applications

Hydrophilic interaction liquid chromatography method for the determination of ascorbic acid

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