Enzymatic, outer membrane proteins and plasmid alterations of starved Vibrio parahaemolyticus and Vibrio alginolyticus cells in seawater

Abdallah, Fethi Ben; Kallel, Héla; Bakhrouf, Amina

doi:10.1007/s00203-009-0477-8

Cited by 27 publications

(23 citation statements)

References 45 publications

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“…alginolyticus and V. parahaemolyticus respond to environmental stress by modifying the rate of synthesis of certain proteins [1]. Alterations observed in the OMPs profiles of UV-C irradiated V. alginolyticus and V. parahaemolyticus were manifested by the appearance of proteins as well as by modifications in the expression level of certain proteins.…”

Section: Discussionmentioning

confidence: 98%

“…Vibrio, a food-borne pathogen, can sense and respond to changes in their external environment [1]. The ability of Vibrio spp.…”

Section: Introductionmentioning

confidence: 99%

“…Several works have shown that when bacteria are transferred to a new environment, the synthesis of their OMPs change [13]. Several alterations were observed in the OMPs profiles of V. alginolyticus and V. parahaemolyticus under starvation conditions [1]. Furthermore, the altered osmolarity of the culturing medium caused changes in the OMP patterns of V. alginolyticus [12] and V. parahaemolyticus [14].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus

et al. 2012

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Vibrio parahaemolyticus and V. alginolyticus, marine foodborne pathogens, were treated with UVCradiation (240 J/m 2 ) to evaluate alterations in their outer membrane protein profiles. Outer membrane protein patterns of UVC-irradiated bacteria were found altered when analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Altered proteins were identified by mass spectrometry (MS and MS/MS) and analysis revealed that OmpW, OmpA, Long-chain fatty acid transport protein, Outer membrane receptor protein, Putative uncharacterized protein VP0167, Maltoporin (lamB), Polar flagellin B/D, Agglutination protein Peptidoglycan-associated lipoprotein and MltA-interacting protein MipA were appeared, thereby they can be considered as UVC-stress proteins in some vibrios. In addition, expression of OmpK decreased to nondetectable level. Furthermore, we observed a decrease or an increase in the expression level of other outer membrane proteins.

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Section: Discussionmentioning

confidence: 98%

“…Vibrio, a food-borne pathogen, can sense and respond to changes in their external environment [1]. The ability of Vibrio spp.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus

et al. 2012

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“…Despite large sets of data [17,21,[23][24][25][26][27] documenting considerable variations in membrane composition of bacterial cells challenged by different stress factors, the nature and specific contribution of many membrane proteins to cell adaptation and survival are still poorly defined. Here, we present a proteomic study addressing changes in the protein composition of the V. harveyi cell envelope under conditions (i.e., long-term permanence in seawater microcosm at 4°C) inducing the VBNC state.…”

Section: Introductionmentioning

confidence: 99%

Changes in the Vibrio harveyi Cell Envelope Subproteome During Permanence in Cold Seawater

et al. 2016

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Previous work demonstrated that physiological, morphological, and gene expression changes as well as the time-dependent entry into the viable but not culturable (VBNC) state are used by Vibrio species to survive and cope with diverse stress conditions including seasonal temperature downshifts and starvation. To learn more about the nature and specific contribution of membrane proteins to cell adaptation and survival, we analyzed variations in the protein composition of cell envelope and related them to morphological and physiological changes that were taking place during the long-term permanence of Vibrio harveyi in seawater microcosm at 4 °C. We found that after 21 days of permanence, nearly all population (ca. 99 %) of V. harveyi acquired the VBNC phenotype. Although the size of V. harveyi cells gradually decreased during the incubation time, we found that this morphological change was not directly related to their entry into the VBNC state. Our proteomic study revealed that the level of membrane proteins playing key roles in cellular transport, maintenance of cell structure, and in bioenergetics processes remained unchanged along starvation at low temperature, thus suggesting that V. harveyi might need these proteins for the long-term survival and/or for the resuscitation process. On a contrary, the level of two proteins, elongation factor Tu (EF-TU) and bacterioferritin, greatly increased reaching the maximal values by the end of the incubation period. We further discuss the above data with respect to the putative roles likely exerted by membrane proteins during transition to and maintaining of the VBNC state.

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“…Lacks of nutrients in natural ecosystem are often limiting factor for microbial population. Viability of starved cells has been largely studied in our laboratory for Salmonella enterica Serovar Typhimurium [25]; Vibrio alginolyticus [26]; Citrobacter [27]; Vibrio fluvialis [28]; Vibrio parahaemolyticus [29] and Shigella spp. [30].…”

Section: Discussionmentioning

confidence: 99%

Starvation survival of Candida albicans in various water microcosms

Chaieb¹,

Kouidhi²,

Zmantar³

et al. 2011

J. Basic Microbiol.

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Candida is a major Human pathogen causing a variety of infections and can survive for extended period of time in aquatic environment including marine and fresh water. In this study we compared a colorimetric XTT assay to colony forming units (CFU) count to evaluate the survival potential of Candida albicans incubated in water microcosms. Our results showed that cells maintain cultivability within a long period followed by a decline in cultivability and a drop of plate counts to less than 20 cell ml(-1) after 150 days in tap water, 190 days in rain water and 200 days in seawater. In addition we noted that 10% of cells viability was reached after 150 days in seawater, 180 days in rain water and 210 days in tap water. Molecular method confirms the persistence of C. albicans cells in water during long time starvation period.

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Enzymatic, outer membrane proteins and plasmid alterations of starved Vibrio parahaemolyticus and Vibrio alginolyticus cells in seawater

Cited by 27 publications

References 45 publications

Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus

Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus

Changes in the Vibrio harveyi Cell Envelope Subproteome During Permanence in Cold Seawater

Starvation survival of Candida albicans in various water microcosms

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