Enzymatic synthesis with mainly undissolved substrates at very high concentrations

Erbeldinger, Markus; Ni, Xiongwei; Halling, Peter J.

doi:10.1016/s0141-0229(98)00039-8

Cited by 83 publications

(47 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Einstein (Investigations on the theory of Brownian movement, 1926) obtained the following theoretical relation for the viscosity of identical non-interacting rigid spherical particle suspensions at low particle volume fractions: Whole cells bacterial 0.5-5.0 (Agerkvist and Enfors, 1990;Bowden, 1985;Harrison, 1991;Hayashi et al, 2001b;Kula et al, 1990) 1,080-1,120 (Erbeldinger et al, 1998;Wong et al, 1997b) 3.0-5.0 (Hayashi et al, 2001a,b Bowden, 1985;Harrison, 1991;Kula et al, 1990;Siddiqi et al, 1996) $1,040 (Lipschutz et al, 2000;Nikolai and Hu, 1992) - Agerkvist and Enfors, 1990;Bowden, 1985;Kula et al, 1990;Wong et al, 1997a;Wong et al, 1997b) 1,061-1,090 (Wong et al, 1997b) $2.8 (Wangsa-Wirawan et al, 2001b)* Yeast 0.05-8.0 (Kula et al, 1990;Siddiqi et al, 1996) --Inclusion bodies 0.05-1.2 (Taylor et al, 1986;…”

Section: Suspension Characteristicsmentioning

confidence: 99%

Strategy for selection of methods for separation of bioparticles from particle mixtures

Hee

Hoeben

Lans

et al. 2006

Biotech & Bioengineering

View full text Add to dashboard Cite

The desired product of bioprocesses is often produced in particulate form, either as an inclusion body (IB) or as a crystal. Particle harvesting is then a crucial and attractive form of product recovery. Because the liquid phase often contains other bioparticles, such as cell debris, whole cells, particulate biocatalysts or particulate by-products, the recovery of product particles is a complex process. In most cases, the particulate product is purified using selective solubilization or extraction. However, if selective particle recovery is possible, the already high purity of the particles makes this downstream process more favorable. This work gives an overview of typical bioparticle mixtures that are encountered in industrial biotechnology and the various driving forces that may be used for particle-particle separation, such as the centrifugal force, the magnetic force, the electric force, and forces related to interfaces. By coupling these driving forces to the resisting forces, the limitations of using these driving forces with respect to particle size are calculated. It shows that centrifugation is not a general solution for particle-particle separation in biotechnology because the particle sizes of product and contaminating particles are often very small, thus, causing their settling velocities to be too low for efficient separation by centrifugation. Examples of such separation problems are the recovery of IBs or virus-like particles (VLPs) from (microbial) cell debris. In these cases, separation processes that use electrical forces or fluid-fluid interfaces show to have a large potential for particle-particle separation. These methods are not yet commonly applied for large-scale particle-particle separation in biotechnology and more research is required on the separation techniques and on particle characterization to facilitate successful application of these methods in industry.

show abstract

Section: Suspension Characteristicsmentioning

confidence: 99%

Strategy for selection of methods for separation of bioparticles from particle mixtures

Hee

Hoeben

Lans

et al. 2006

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…At the same time, the excess of oleyl alcohol will hinder the interaction frequency between substrate and lipases (Kanasawud et al 1992;Claon and Akoh, 1994). This is may be due to the presence of high substrates concentration, the viscosity of the reaction mixture surrounding the enzyme molecule may be increased due to the increase in the alcohol leading to ineffective mixing of reactants and subsequent reduction in reaction rate (Erbeldinger et al 1998).This is contrary to what was reported by Chen et al 1995. The optimal molar ratio to produce oleyl oleate was 1:1.…”

Section: Effect Of Molar Ratio Of Substratesmentioning

confidence: 99%

High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica

Radzi¹,

Basri²,

Salleh³

et al. 2005

Electron. J. Biotechnol.

View full text Add to dashboard Cite

“…Mixing and mass transfer in SFS are factors to heed, considering the high viscosity of these media. The poor solubility of fatty acids with a low melting point can be a limitation, but an increase in temperature to melt the acid can be a solution (Erbeldinger et al, 1998(Erbeldinger et al, , 1999.…”

Section: Introductionmentioning

confidence: 99%

Esterification by immobilized lipase in solvent‐free media: Kinetic and thermodynamic arguments

Sandoval

Condoret

Monsan

et al. 2002

Biotech & Bioengineering

View full text Add to dashboard Cite

The aim of this study is to characterize, in solvent-free systems (SFS), the kinetic and thermodynamic performance of batch lipase-catalyzed esterification. SFS are compared to a conventional organic solvent, n-hexane. The esterification of oleic acid with ethanol was chosen as a model reaction. The TABEK (thermodynamic activity-based enzyme kinetics) approach was used to rationally analyze kinetics. Influence of the reaction medium on final conversions was also studied. Several factors, such as initial molar ratio of substrates, reactant availability, initial water content, and quantity of immobilized enzyme, were examined. Special attention was also turned to enzyme stability and reuse after reaction, this last item being a prerequisite in the development of industrial processes. SFS proved to be almost as efficient as n-hexane from a kinetic and thermodynamic point of view and offered a better volumetric production.

show abstract

Enzymatic synthesis with mainly undissolved substrates at very high concentrations

Cited by 83 publications

References 24 publications

Strategy for selection of methods for separation of bioparticles from particle mixtures

Strategy for selection of methods for separation of bioparticles from particle mixtures

High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica

Esterification by immobilized lipase in solvent‐free media: Kinetic and thermodynamic arguments

Contact Info

Product

Resources

About