Enzyme-Based Biosensors and Their Applications

Fan, Yufan; Guo, Zhao-Bin; Ge, Guangbo

doi:10.3390/bios13040476

Cited by 10 publications

(3 citation statements)

References 20 publications

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“…The configuration, performances, and applications of enzyme-based biosensors were discussed in several recent reviews [ 6 , 8 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 ]. Both the direct catalytic activity and the inhibition of enzymatic activity were exploited in these applications [ 6 , 8 , 76 , 90 ].…”

Section: Characteristics Of Extremozyme-based Biosensors For Environm...mentioning

confidence: 99%

Extremozyme-Based Biosensors for Environmental Pollution Monitoring: Recent Developments

Purcarea,

Ruginescu,

Banciu

et al. 2024

Biosensors

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Extremozymes combine high specificity and sensitivity with the ability to withstand extreme operational conditions. This work presents an overview of extremozymes that show potential for environmental monitoring devices and outlines the latest advances in biosensors utilizing these unique molecules. The characteristics of various extremozymes described so far are presented, underlining their stability and operational conditions that make them attractive for biosensing. The biosensor design is discussed based on the detection of photosynthesis-inhibiting herbicides as a case study. Several biosensors for the detection of pesticides, heavy metals, and phenols are presented in more detail to highlight interesting substrate specificity, applications or immobilization methods. Compared to mesophilic enzymes, the integration of extremozymes in biosensors faces additional challenges related to lower availability and high production costs. The use of extremozymes in biosensing does not parallel their success in industrial applications. In recent years, the “collection” of recognition elements was enriched by extremozymes with interesting selectivity and by thermostable chimeras. The perspectives for biosensor development are exciting, considering also the progress in genetic editing for the oriented immobilization of enzymes, efficient folding, and better electron transport. Stability, production costs and immobilization at sensing interfaces must be improved to encourage wider applications of extremozymes in biosensors.

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Section: Characteristics Of Extremozyme-based Biosensors For Environm...mentioning

confidence: 99%

Extremozyme-Based Biosensors for Environmental Pollution Monitoring: Recent Developments

Purcarea,

Ruginescu,

Banciu

et al. 2024

Biosensors

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show abstract

“…Enzymes not only have been used for triggering biochemical cascade reactions, environmental monitoring, and food quality control, but also as biomarkers for human diseases which implies a huge potential for medical diagnosis [107][108][109]. Thus, these molecules are being used for the development of enzyme-based biosensors for detecting target analytes in complex systems [110].…”

Section: Enzymes-based Biosensormentioning

confidence: 99%

Trends on Aerogel-Based Biosensors for Medical Applications: An Overview

Almeida,

Merillas,

Pontinha

2024

IJMS

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Aerogels are unique solid-state materials composed of interconnected 3D solid networks and a large number of air-filled pores. This structure leads to extended structural characteristics as well as physicochemical properties of the nanoscale building blocks to macroscale, and integrated typical features of aerogels, such as high porosity, large surface area, and low density, with specific properties of the various constituents. Due to their combination of excellent properties, aerogels attract much interest in various applications, ranging from medicine to construction. In recent decades, their potential was exploited in many aerogels’ materials, either organic, inorganic or hybrid. Considerable research efforts in recent years have been devoted to the development of aerogel-based biosensors and encouraging accomplishments have been achieved. In this work, recent (2018–2023) and ground-breaking advances in the preparation, classification, and physicochemical properties of aerogels and their sensing applications are presented. Different types of biosensors in which aerogels play a fundamental role are being explored and are collected in this manuscript. Moreover, the current challenges and some perspectives for the development of high-performance aerogel-based biosensors are summarized.

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“…Near-infrared (NIR) probes possess excellent penetration depth and improved signal-to-noise ratio (SNR), which immensely facilitates cellular imaging and cell-based drug discovery. , To the best of our knowledge, the practical and highly specific NIR probes for hCES2A are rarely reported, either because some proposed recognition groups fail to quench the fluorophore thoroughly or the designed NIR substrates can also be hydrolyzed by other serine hydrolases . As such, it is necessary to design practical hCES2A probes with good photophysical properties and ER-accumulation ability, which will strongly facilitate in situ imaging and high-throughput inhibitor screening of this key ER-resident enzyme. − …”

mentioning

confidence: 99%

Rationally Engineered hCES2A Near-Infrared Fluorogenic Substrate for Functional Imaging and High-Throughput Inhibitor Screening

Fan,

Zhang,

Song

et al. 2023

Anal. Chem.

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Human carboxylesterase 2A (hCES2A) is an important endoplasmic reticulum (ER)-resident enzyme that is responsible for the hydrolytic metabolism or activation of numerous ester-bearing drugs and environmental toxins. The previously reported hCES2A fluorogenic substrates suffer from limited emission wavelength, low specificity, and poor localization accuracy, thereby greatly limiting the in situ functional imaging of hCES2A and drug discovery. Herein, a rational ligand design strategy was adopted to construct a highly specific near-infrared (NIR) substrate for hCES2A. Following scaffold screening and recognition group optimization, HTCF was identified as a desirable NIR fluorophore with excellent photophysical properties and high ER accumulation ability, while several HTCF esters held a high potential to be good hCES2A substrates. Further investigations revealed that TP-HTCF (the tert-pentyl ester of HTCF) was an ideal substrate with ultrahigh sensitivity, excellent specificity, and a substantial signal-to-noise ratio. Upon the addition of hCES2A, TP-HTCF could be rapidly hydrolyzed to release HTCF, a chemically stable product that emitted bright fluorescent signals at around 670 nm. A TP-HTCF-based biochemical assay was then established for the high-throughput screening of potent and cell-active hCES2A inhibitors from an in-house compound library. Furthermore, TP-HTCF displayed high imaging resolution for imaging hCES2A in living cells as well as mouse liver slices and tumor-xenograft mice. Collectively, this study demonstrates a rational strategy for developing highly specific fluorogenic substrates for an ER-resident target enzyme, while TP-HTCF can act as a practical tool for sensing hCES2A in living systems.

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Enzyme-Based Biosensors and Their Applications

Abstract: Enzymes constitute an extremely important class of biomacromolecules with diverse catalytic functions, which have been validated as key mediators for regulating cellular metabolism and maintaining homeostasis in living organisms [...]

Cited by 10 publications

References 20 publications

Extremozyme-Based Biosensors for Environmental Pollution Monitoring: Recent Developments

Extremozyme-Based Biosensors for Environmental Pollution Monitoring: Recent Developments

Trends on Aerogel-Based Biosensors for Medical Applications: An Overview

Rationally Engineered hCES2A Near-Infrared Fluorogenic Substrate for Functional Imaging and High-Throughput Inhibitor Screening

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