Enzyme‐based detergent formulas for fatty soils and hard surfaces in a continuous‐flow device

Abstract

The present work analyzes the effect of incorporating a lipolytic enzyme (Lipolase ® 100L) into detergent formulas for washing fatty soils on hard surfaces. The experimental device, which is called a "bath-substrate-flow" device, uses a continuous flow on a substrate (glass spheres) soiled with triolein. Washing tests were done using only the enzyme and changing both its concentration and the temperature of the process. The results showed that, in the presence of lipase, soil removal was achieved through three consecutive mechanisms: (i) fundamental removal of the soil by the bath flow through the experimental device; (ii) emulsion of the soil in the washing medium; and (iii) enzymatic hydrolysis of the dispersed soil. Different commercial surfactants were used, and detergency was evaluated in the absence and presence of lipase. The use of surfactant formulas with the lipolytic enzyme showed a positive effect of the enzyme on the detergency values registered with the fatty alcohol ethoxylate surfactants Findet ® 10/15 and Findet 1214N/23, and with the anionic surfactant linear alkylbenzene sulfonate. The commercial surfactants Glucopon ® 600, Glucopon 650, Findet 10/18, and Findet Q/21.5NF alone each presented high detergency values for fatty soils, and the effect of the incorporation of the lipase was not significant.Paper no. S1493 in JSD 9, 83-90 (Qtr. 1, 2006).

Hard‐Surface Cleaning Using Lipases: Enzyme–Surfactant Interactions and Washing Tests

“…This can be explained because tributyrin is a relatively easy material to remove and disperse, due to its physicochemical properties, low surface tension and log P value (see Table 1). However, in a previous study [10] carried out with triolein, which is harder to remove than tributyrin, detergency increased by 11.8% when the surfactant LAS (1 g/L) was used together with the enzyme Lipolase 100 (0.1 g/L) compared to the detergency registered in the absence of the enzyme.…”

“…A simplified scheme of this device is shown in Fig. 1, whereas a full description of the same can be found elsewhere [10,11]. Once soiled, the substrate, [14] 36.0 mN/m (25°C) Log P a 23.7 composed of borosilicate glass beads 3 mm in diameter, is placed inside a column (B).…”

“…The concentration of oleic acid in the washing bath was determined spectrophotometrically, following the procedure described elsewhere [10]. The interference of surfactants in the extraction and spectrophotometric determination of both the tributyrin and the oleic acid was studied.…”

“…In a previous study [10], the mechanism of stain removal from hard surfaces in the presence of lipases was investigated using a continuous-flow device [11]. However, research on lipase-based detergent formulas needs more attention in order to clarify such aspects as the interaction of lipases with surfactants in the presence of an oily phase, or the influence of temperature and enzyme concentration on the performance of lipases under washing conditions.…”

Evaluation of Detergency Using Artificially Soiled Multifiber Fabrics

“…The development of eco-friendly and high energy efficiency detergent systems is an important social issue [8][9][10][11] . For the purpose, the exact evaluation of detergency is necessary and hence well-defi ned model systems have been proposed to clarify the detergency mechanism [12][13][14][15][16][17][18][19] . However, conventional evaluating methods with artifi cially soiled fabrics are needed because the fabric has complicated geometrical structure [20][21][22][23][24][25][26][27][28] .…”